2001 Fiscal Year Final Research Report Summary
The investigation of the relationship between cell death in neurodegerative disorders and ultra trace elements
| Project/Area Number |
12680771
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Neuroscience in general
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| Research Institution | KYOTO UNIVERSITY |
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Principal Investigator |
IDE Ari International Innovation Center, Kyoto University, Professor, 国際融合創造センター, 教授 (50232939)
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| Co-Investigator(Kenkyū-buntansha) |
YOSHIDA Souhei Kansai College of Oriental Medicine, Professor, 教授 (30166954)
KAWAI Jun Graduate School of Engineering, Kyoto University, Professor, 工学部, 教授 (60191996)
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| Project Period (FY) |
2000 – 2001
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| Keywords | Micro beam / Brain Function / Neuron Degeneration / Parkinson's Disease / Alzheimer's Disease / Synchrotron Radiation / Chemical State Analysis / Elemental Image |
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| Research Abstract |
The purpose of this study was to take a new approach about the mechanism of neurodegenerative disorder and cell death by combining the quantitative information of trace metal elements and histological observation at the single cell level. The information obtained in this analysis is significant not only from the viewpoint of therapeutic and medical applications, but also the basic biology concerning the cell death and function of proteins. As the typical neurodegenarative disorders, parkinsonism-dementia complex (PDC) and amyotrophic lateral sclerosis (ALS) are investigated in the present study. The pathology of these diseases is extremely complex and the decisive mechanism responsible for the neuronal cell death remains unelucidated. It is expected that innovative knowledge about these disorders be obtained through the high sensitivity elemental analysis.
1. Biopsies were obtained by autopsy.
2. The elemental imaging and quantitative analysis were performed using Synchrotron radiation (SR) X-ray spectroscopy analysis.
3. The chemical state imaging of neurons was performed using X-ray absorption fine structure spectroscopy.
The experimental results were analyzed pathologically, indicating that there are considerable correlation between trace elements and neurodegenerative disorders. These, results were published in several international journals.
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