Construction of congenic strains from epileptic model rat NER

2003 Fiscal Year Final Research Report Summary

• Project/Area Number: 12680810
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Laboratory animal science
• Research Institution: Hokkaido University (2002-2003); Kyoto University (2000-2001)
• Principal Investigator: KITADA Kazuhiro Hokkaido University, Center for Advanced Science and Technology, Associate professor, 先端科学技術共同研究センター, 助教授 (70263093)
• Co-Investigator(Kenkyū-buntansha): SERIKAWA Tadao Kyoto University, Graduate School of Medicine, Professor, 医学研究科, 教授 (30025655)
• Project Period (FY): 2000 – 2003
• Keywords: epilepsy / animal model / NER / genetic analysis / congenic strain

Research Abstract

NER is an animal model of epilepsy that exhibit spontaneous tonic-clonic convulsions, beginning with neck and forelimb clonus, wild jumping/running, opisthotonic posturing, and evolving to tonic, then clonic convulsion, followed by postictal flaccidity. Ictal cortical and hippocampal EEGs are characterized by high-voltage spikes followed by diffuse spike-and-wave or polyspike-and-wave complexes. Seizure susceptibility to pentylenetetrazol, tossing, and transcorneal electroshock, but not to tactile, photic, or acoustic stimuli, or to transauricular electroshock. No pathologic changes were demonstrated in the CNS. Spontaneous convulsions are controlled by a major autosomal recessive gene for epilepsy, that are comparable to generalized tonic-clonic seizures in humans
In this project, we have generated two congenic lines of F344.NER-ner1^<NER>/ner1^<NER> and NER.F344-ner1^<F344>/ner1^<F344>. The former is expected to to show GTC, and the latter is expected not to show GTC. In the future, it is considered that strict animal experiment for reseach on epilepsy will be possible using NER, F344 and these 2 lines of congenic strains.

Research Products

• [Journal Article] Involvement of Ca2+ Channels in abnormal excitability of hippocampal CA3 pyramidal cells in Noda epileptic rats (2003)
  • Author(s): Kiura, Y.ら
  • Journal Title: J Pharmacol Sci 91(2) Pages: 137-144
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Identification and distribution of aspartoacylase in the postnatal rat brain (2003)
  • Author(s): Klugmann, M.ら
  • Journal Title: Neuroreport 14(14) Pages: 1837-1840
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Activation by N-acetyl-L-aspartate of acutely dissociated hippocampal neurons in rats via metabotropic gluamate receptors (2003)
  • Author(s): Yan, H-D.ら
  • Journal Title: Epilepsia 44(9) Pages: 1153-1159
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Identification and distribution of aspartoacylase in the postnatal rat brain. (2003)
  • Author(s): Klugmann, M., Symes, CW., Klaussner, BK., Leichitlein, CB., Serikawa, T., Young, D., During, MJ
  • Journal Title: Neuroreport. 14(14) Pages: 1837-1840
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Activation by N-acetyl-L-aspartate of acutely dissociated hippocampal neurons in rats via metabotropic gluamate receptors. (2003)
  • Author(s): Yan, H-D., Ishihara K., Serikawa, T., Sasa, M.
  • Journal Title: Epilepsia. 44(9) Pages: 1153-1159
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Involvement of Ca^<2+> Channels in abnormal excitability of hippocampal CA3 pyramidal cells in Noda epileptic rats. (2003)
  • Author(s): Kiura, Y., Hanaya, R., Serikawa, T., Kurisu, K., Sakai, N., Sasa, M.
  • Journal Title: J Pharmacol Sci 91 Pages: 137-144
  • Description: 「研究成果報告書概要(欧文)」より