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2002 Fiscal Year Final Research Report Summary

The Model Study of Vaccine Development against Cysticercosis by Expression-Library Immunization using Taenia taeniaeformis-rat system

Research Project

Project/Area Number 12680811
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Laboratory animal science
Research InstitutionTottori University

Principal Investigator

OKAMOTO Munehiro  Tottori University, Facul.of Agriculture, Assoc.Prof, 農学部, 助教授 (70177096)

Co-Investigator(Kenkyū-buntansha) ITO Akira  Asahikawa Med.Col., Facul.of Medicine, Prof., 医学部, 教授 (70054020)
SHIBAHARA Toshiyuki  Tottori University, Facul.of Medicine, Assoc.Prof, 医学部, 助教授 (70116937)
Project Period (FY) 2000 – 2002
KeywordsTaenia taeniaeformis / Library Vaccine / rat / Protective Antigen / immunity / Taeniid Cestode
Research Abstract

Cysticercosis, one of the most serious larval cestodiasis of medical importance in humans and of economic importance in pigs, is spreading worldwide, especially in Asia, Africa and Latin America. The trial of the vaccine development against Taenia solium cysticercosis has been made in many countries, and actually some candidate molecules have been also reported. However, the effects of vaccine used those molecules have been not always perfect. Expression-library immunization has been, proposed as an effective means to screen a large number of genes of the pathogen as candidate protective molecules. In this study, we examined the efficacy of expression-library immunization for vaccine development against T.solium cysticercosis using T.taeniaeformis-rat model system
Total RNAs were isolated from last 10 segments of adult T.taeniaeformis by Isogen LS, and poly A RNA was purified using oligotex-dT30 (Takara). cDNA library was produced using SuperScript Plasmid System (Inbitorogen), which contain a mammalian expression vector, pCMV・SPORT6. Transformed E.coli were plated onto LB-plate containing ampicillin, and the cells that grew on the plate were resuspended in TYGPN nedium. Each clone was sequenced and classified with the property of the insert. Twenty to thirty independent clones were simultaneously propagated in LB medium with ampicillin, and plasmid DNAs were isolated and purified using End Free Plasmid Kit. (QIAGEN). For the immunization, purified plasmid DNAs were injected directly into the spleen and muscle of mice
It was reported that the number of the mitochondrion in adult taeniid cestode was not so many. However, more than 30% of the sequenced clones had mitochondrial genes. Specific antibody to Taenia taeniaeformis was detected by western-blot in the mouse immunized with Library-Vaccine

  • Research Products

    (14 results)

All Other

All Publications (14 results)

  • [Publications] Ito A., Yamasaki H., et al.: "Multiple genotypes of Taenia solium-ramifications for diagnosis, treatment control"Acta Tropica. 87. 95-101 (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Margono S.S., Ito A., et al.: "Taenia solium taeniasis/cysticercosis in Papua, Indonesia in 2001 : detection of human worm carriers"Journal of Helminthology. 77. 39-42 (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ito A., Putra M., et al.: "Dogs as alternative intermediate hosts of Taenia solium in Papua (Irian Jaya),Indonesia confirmed by highly specific ELISA and immunoblot using native and recombinant antigens and mitochondrial DNA analysis"Journal of Helminthology. 76. 311-314 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yamasaki H., Nakano M., et al.: "DNA differential diagnosis of human taeniid cestodes by base excision sequence scanning thymine-base reader analysis with mitochondrial genes"Journal of Clinical Microbiology. 40. 3818-3821 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nakao M., Okamoto M., et al.: "A phylogenetic hypothesis for the distribution of two genotypes of the pig tapeworm Taenia solium worldwide"Parasitology. 124. 657-662 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Okamoto M., Nakao M., et al.: "Molecular Variation of Taenia solium in the World"Southeast Asian Journal of Tropical Medicine and Public Health. 32,Supple2. 90-93 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ito A., Nakao M., et al.: "Taenia solium Cysticercosis (eds G.Singh, S.Prabhakar)"Mitochondrial DNA of Taenia solium : from basic to applied science. 9 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ito A., Yamasaki H., Nakao M., Sako Y., Okamoto M.et al.: "Multiple genotypes of Taenia solium-ramifications for diagnosis, treatment and control"Acta Tropica. 87. 95-101 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Margono S.S., Ito A., Sato M.0., Okamoto M.et al.: "Taenia solium taeniasis/cysticercosis in Papua, Indonesia in 2001 : detection of human worm carriers"Journal of Helminthology. 77. 39-42 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ito A., Putra M.I., Subahar R., Sato M.0., Okamoto M.et al.: "Dogs as alternative intermediate ghosts of Taenia solium in Papua (Irian Jaya), Indonesia confirmed by highly specific ELISA and immunoblot using native and recombinant antigens and mitochondrial DNA analysis"Journal of Helminthology. 76. 311-314 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yamasaki H., Nakao M., Sako Y., Nakaya K., Sato M.O., Mamuti W., Okamoto M., Ito A.: "DNA differential diagnosis of human taeniid cestodes by base excision sequence scanning thymine-base reader analysis with mitochondrial genes"Journal of Clinical Microbiology. 40. 3818-3821 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nakao M., Okamoto M., Sako Y., et al.: "A phylogenetic hypothesis for the distribution of two genotypes of the pig tapeworm Taenia solium worldwide"Parasitology. 124. 657-662 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Okamoto M., Nakao M., Sako Y., Ito A.: "Molecular Variation of Taenia solium in the World"Southeast Asian Journal of Tropical Medicine and Public Health. 32, Suppl.2. 90-93 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ito A., Nakao M., et al.: "Mitochondrial DNA of Taenia solium : from basic to applied science"Taenia solium Cycticercosis (eds G.Singh and S.Prabhakar). (2002)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2005-04-19  

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