2001 Fiscal Year Final Research Report Summary
CLINICAL AND EXPERIMENTAL RESEARCH ABOUT THE EFFECT OF NEW AUTONOMC DRUGS FORNEUROGENIC BLADDER
| Project/Area Number |
12832038
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Institution | Kagoshima University |
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Principal Investigator |
TANAKA Nobuyuki Kagoshima University, Faculty of Medicine, Professor, 医学部, 教授 (40041454)
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| Co-Investigator(Kenkyū-buntansha) |
IKEDA Satoshi Kagoshima University, Faculty of Medicine, Research Associate, 医学部・附属病院, 助手
ETOH Seiji Kagoshima University, University Hospital, Faculty of Medicine, Assistant Professor, 医学部・附属病院, 講師 (70295244)
KAWAHIRA Kazumi Kagoshima University, Faculty of Medicine, Associate Professor, 医学部, 助教授 (20117493)
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| Project Period (FY) |
2000 – 2001
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| Keywords | Spinal Cord Injury / Rat / Spinal Cord / Urinaiy Bladder / Endothelin / RIA / Cystometry |
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| Research Abstract |
Functional role of endothelin receptor in urinary bladder and spinal cord were examined in spinal cord injured rats. Female Sprague-Dawley rats were spinalized at T3-5 level. After 2-3 weeks maintenance, following experiments were performed.
1.Mesurement ofendothelin-1 level in die urinary bladder.(RIA) The concentration of endothelin-1(ET-1) in the urinary bladder of control and spinal cord injured rats was 395 ± 49 and 234 ± 27 pg/g wet weight, respectively.
2. Mesurement of mechanical activity of detrusor strips. ET-1 induced dose-dependent contraction. Both ET_A receptor antagonist BQ123 and ET_B receptor antagonist BQ788 innhibited the response by ET-1: ET_B receptor agonist sarafotoxinS6c had very weak contractile activiy. There is no difference of contractile property of endothellin- 1 and SarafotoxinS6c between control rats and spinal cord injured rats.
3. Cystometry The effect of intrathecal administration of ET-1 and sarafotoxinS6c on micturition reflex of spinal cord injured rats were examined. Intrathecal administration of 100pmol ET-1 inhibited micturition reflex. But 100pmol sarafotoxinS6c had no inhibitory effect on micturition reflex. These results indicate that the inhibitory eftect of ET- 1 on micturition reflex is mediated by spinal ET_A receptor.
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