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2002 Fiscal Year Final Research Report Summary

Enzymatic Conversion of Sweetpotato Starch Residue into New Food Materials

Research Project

Project/Area Number 12839015
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 生物資源の変換と展開
Research InstitutionKagoshima University

Principal Investigator

SUGANUMA Toshihiko  Kagoshima University, FAC. AGRICULTURE, PROFESSOR, 農学部, 教授 (90117515)

Project Period (FY) 2000 – 2002
KeywordsSweetpotato / Starch residue / Enzymatic degradation / Dietary fiber / Sugar Analysis / Pectinase / Arabinanase / Carbohydrate resources
Research Abstract

The manufacture of starch from tropical rootcrops like sweetpotato (SP) and cassava (CA) produce considerable amounts of starch residue as by-product. This residue is rich in cell wall material (CWM) which makes it a potential source of dietary fiber. This study characterized the CWMs obtained from the starch residue of three rootcrops, SP, CA, and potato (PO) in terms of their sugar composition and attempted to convert them into soluble dietary fiber enzymatically.
To characterize the CWMs from the rootcrops, their sugar compositions were analyzed first. For relatively insoluble samples, pretreatment with 12 M H_2SO_4 prior to hydrolysis with 1 M H_2SO_4 was required for complete hydrolysis. For monosaccharide analysis, HPAEC-PAD method with a CarboPac PA10 column, instead of a PA1 column resulted in reliable sugar composition of these samples. Using this method of analysis, the sugar composition of the CWMs and their polysaccharide fractions was determined. Among the polysaccharide fr … More actions, it was only in the hemicellulose fraction where significant differences in the sugar composition, especially in the galactose content were observed among the three rootcrops. This has been the first report on the sugar composition of CA CWM.
To convert the CWMs into soluble dietary fiber, a bacteria producing a CWM-degrading enzyme was isolated from soil, Bacillus sp. M4, and the crude enzyme solution from its culture filtrate was used to solubilize the residues. Bacillus sp. M4 has been found to secrete polygalacturonic acid lyase and glycan depolymerase activities especially arabinanase, but cellulase activity was nearly absent. Sugar analysis of the solubilized product from the CWM of the three rootcrops revealed that it is mainly composed of galacturonic acid, and the neutral sugars found commonly in the pectin fraction, suggesting the presence of a protopectinase activity in M4 enzyme. The mode of action of the crude enzyme was determined by terminal sugar analysis using HPAEC-PAD after hydrolysis of the reduced products. Results revealed that M4 enzyme attacked the galactan as well as rhamnogalacturonan moieties of the protopectin, resulting in the release of a soluble dietary fiber of pectin fraction. Less

  • Research Products

    (10 results)

All Other

All Publications (10 results)

  • [Publications] Salvador, L.D., Suganuma, T., Kitahara, K., Tanoue, H., Ichiki., M.: "Monosaccharide composition of sweetpotato fiber and cell wall polysaccharides from sweetpotato, cassava and potato analyzed by HPAEC-PAD method"Journal of Agricultural Food Chemistry. 48(8). 3448-3454 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kitahara, K., Antoku, M., Hori, Y., Sedoshita, A., Suganuma., T: "Developmental change in starch granules in sweetpotato callus"Carbohydrate Polymers. 49. 91-96 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Salvador, L.D., Suganuma, T., Kitahara, K., Fukushige, Y., Tanoue, H: "Degradation of cell wall materials from sweetpotato, cassava, and potato by a bacterial protopectinase and terminal sugar analysis of the resulting solubilized products"J.Biosci.Bioeng.. 93(1). 64-72 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 菅沼俊彦: "J.Applied Glycoscience"日本応用糖質科学会. 7 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Lorena Salvador, T.Suganuma., K.Kitahara, H.Tanoue, M.Ichiki: "Potential of root crops for food and industrial resources"Organizing Committee of the International Society for Tropical Root Crops (ISTRC), Tsukuba. 5 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Salvador, L.D., Suganuma, T., Kitahara, K., Tanoue, H. and Ichiki, M.: "Monosaccharide composition of sweetpotato fiber and cell wall polysaccharides from sweetpotato, cassava and potato analyzed by HPAEC-PAD method"Journal of Agricultural Food Chemistry. 48(8). 3448-3454 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kitahara, K., Antoku, M., Hori, Y., Sedoshita, A., and Suganuma, T.: "Development change in starch granules in sweetpotato callus"Carbohydrate Polymers. 49. 91-96 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Salvador, L.D., Suganuma, T., Kitahara, Fukushige, Y., and Tanoue, H.: "Degradation of cell wall materials from sweetpotato, cassava, and potato by a bacterial protopectinase and terminal sugar analysis of the resulting solubilized products"J. Biosci. Bioeng.. 93(1). 64-72 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Suganuma, T.: "Study on two types of alpha-amylase, the acid-stable and the neutral"J. Applied Glycoscience. 48(2). 187-193 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Salvador, L.D., Suganuma, T., Kitahara, K., Tanoue, H. and Ichiki, M.: "Sugar composition of sweetpotato fiber and cell wall polysaccharides from sweetpotato, cassava and potato by HPAEC-PAD method, in Potential of Root crops for food and industrial resources"the organizing committee of the international society for tropical root crops (ISTRC2000). 280-284 (2002)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2004-04-14  

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