2003 Fiscal Year Final Research Report Summary
Application of Hydrophobic Interaction among Biomolecules under Extreme Conditions to Strongly-correlated Softmaterials
| Project/Area Number |
13031024
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| Research Category |
Grant-in-Aid for Scientific Research on Priority Areas
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| Allocation Type | Single-year Grants |
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| Review Section |
Science and Engineering
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| Research Institution | Tokyo Institute of Technology |
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Principal Investigator |
NAKAMURA Satoshi Tokyo Tech, Bioeng, Professor, 大学院・生命理工学研究科, 教授 (50227899)
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| Project Period (FY) |
2001 – 2003
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| Keywords | Bioengineering / Biotechnology / Softmaterial / Protein / Extreme environment |
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| Research Abstract |
The objective of this research is to elucidate the importance of non-covalent bonds in proteins on tolerances towards extreme environments. A 2Fe-2S feredoxin from triangular disc-shaped extremely halophilic archaeon Haloarcula japonica was investigated. It seemed that the additional amino terminal region of H. japonica Fd was importantfor halotolerancy. The thermostability of 3-isopropylmalate dehydrogenase of moderate facultative thermophile Bacillus coagulans was improved by amino acid substitutions at the subunit-subunit interface and/or a loop region.Furthermore, the function of chitin-binding domain of a multi-domain chitinase from alkaliphilic Bacillus sp. J813 was elucidated.
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Research Products
(14 results)
