2001 Fiscal Year Annual Research Report

初期発生制御システムの包括的解析

Research Project

Project/Area Number	13044003
Research Category	Grant-in-Aid for Scientific Research on Priority Areas (A)
Research Institution	Okazaki National Research Institutes
Principal Investigator	上野直人岡崎国立共同研究機構, 基礎生物学研究所, 教授 (40221105)
Co-Investigator(Kenkyū-buntansha)	小林悟岡崎国立共同研究機構, 統合バイオサイエンスセンター, 教授 (90225508) 西田宏記東京工業大学, 生命理工学部, 助教授 (60192689) 平良眞規東京大学, 大学院・理学系研究科, 助教授 (60150083) 田代康介九州大学, 大学院・農学研究院, 助教授 (00192170)
Keywords	初期発生 / 形態形成 / 細胞間相互作用 / シグナル伝達 / データベース / マイクロアレイ / 遺伝子発現制御 / パターン形成
Research Abstract	発生・再生現象を理解するためには、時間軸に沿った遺伝子発現の詳細を明らかにする必要がある。そのためには遺伝子発現プロフィールの網羅的記載を基に動物種を超えて発生生物学研究に共通に活用されるべき遺伝子発現データベースを構築することに加え,発現プロフィールから遺伝子制御の階層性およびネットワークを予測することを試みることも必要である。第1班においては本年度ホヤcDNAデータベース(東工大・西田)、時間的・空間的遺伝子発現解析を含むアフリカツメガエル頭部特異的遺伝子ESTデータベース(東大・平良)、約70,000配列を収集したESTデータベース(基礎生物学研究所・上野)などについては公開まで進展し、本研究の目的を達成しつつあるとともに、遺伝子を高密度に配列させて遺伝子発現プロフィールを解析するDNAアレイ法を確立するなど、研究基盤の確立において国際的にも大きな貢献をした。現在のDNAアレイ法の問題点として遺伝子発現解析の細胞レベルの解像度(空間分解能)がまだ十分でないことが上げられたが九州大学田代らによってRNAポリメラーゼを用いてRNAを増幅する方法が確立されつつあり、小さな胚を扱う研究者に朗報をもたらした。さらに,網羅的解析から初期発生を制御すると予想された遺伝子やタンパク質の機能を解明するためのハイスループット機能解析系、cDNAライブラリーから形態形成を制御する遺伝子をスクリーニングする発現クローニングが成確立され、興味ある遺伝子が同定されつつある。

Research Products

(30 results)

All Other

All Publications (30 results)

[Publications] Kurata, T., Nakabayashi, J., Yamamoto, T.S., Mochii, M., Ueno, N: "Visualization of endogenous BMP signaling during Xenopus development"Differentiation. 67. 33-40 (2001)
[Publications] Ishizuya, A., Ueda, S., Amano, T., Shimizu, K., Suzuki, K., Ueno, N., Yoshizato, K.: "Thyroid-hormone-dependent and fibroblast-specific expression of BMP-4 correlates With adult epithelial development during amphibian intestinal remodeling"Cell Tissues Res.. 303. 187-195 (2001)
[Publications] Morita, K., Shimizu, M., Shibuya, H., Ueno, N.: "A Daf-1-binding protein BRA-1 ia a negative regulator of Daf-7 TGF-b signaling"Proc.Natl.Acad.Sci.USA. 98. 6284-6288 (2001)
[Publications] Hanazawa, M., Mochii, M., Ueno, N., Kohara, Y., Iino, Y.: "Use of cDNA subtraction and RNA interference screens in combination reveals Genes required for germ-line development in Caenohabditis elegans"Proc.Natl.Acad.Sci.USA. 98. 8686-8691 (2001)
[Publications] Yamamoto, T.S., Takagi, C., Hyodo, A., Ueno, N.: "Suppression of head formation by Xmsx-1 through the inhibition of intracellular nodal signaling"Development. 128. 2769-2779 (2001)
[Publications] Sugawara, K., Morita, K., Ueno, N., Shibuya, H.: "BIP, a BRAM-interacting protein involved in TGF-beta signaling, regulates body length in Caenorhabditis elegans"Gene Cells. 6. 566-606 (2001)
[Publications] Yoshida, S., Morita, K., Mochii, M., Ueno, N.: "Hypodermal expression of Caenorhabditis elegans TGF-b type I receptor SMA-6 is essential for the growth and maintenance of body length"Dev.Biol.. 240. 32-45 (2001)
[Publications] Ohkawwara, B., Iemura, S.-I., ten Dijke, P., Ueno, N.: "Action range of BMP is defined by its N-terminal basic amino acid core"Curr.Biol.. 12. 205-209 (2002)
[Publications] H.Sano, M.Mukai, S.Kobayashi: "Maternal Nanos and Pumilio regulate zygotic vasa expression autonomously in the germline progenitors of Drosophila"Develoop.Growth & Differ.. 43. 545-552 (2001)
[Publications] A.Nakamura, R.Amikura, K.Hanyu, S.Kobayashi: "Me 31B silences translation of oocyte-loclizing RNAs through the formation of cytoplasmic RNP complex during Drosophila oogenesis"Development. 128. 3233-3242 (2001)
[Publications] M.Kashikawa, R.Amikura, S.Kobayashi: "Mitochondrial small rribosomal RNA is a component of germinal granules in Xenopus embryos"Mech.Dev.. 101. 71-77 (2001)
[Publications] R.Amikura, K.Hanyu, M.Kashikawa, S.Kobayashi: "Tudor protein is essential for the localization of mmitochondrial ribosomal RNAs in Polar granules in germ plasm of Drosophila embryos"Mech.Dev.. 107. 97-104 (2001)
[Publications] R.Amikura, M.Kashikawa, A.Nakamura, S.Kobayashi: "Presence of Mitochondrial-type ribosomes outside mitochondria in germ plasm of Drosophila embryos"Proc.Natl.Acad.Sci.USA. 98. 9133-9138 (2001)
[Publications] Shinga, J., Itoh, M., Shiokawa, K., Taira, S., Taira, M.: "Early patterning of the prospective midbrain-hindbrain boundary by the HES-Related gene XHR 1 in Xenopus embryo"Mech.Dev.. 109. 225-239 (2001)
[Publications] Shibata, M., Itoh, M., Ohmori, S.-Y., Shinga, J., Taira, M.: "Systematic screening and expression analysis of the head organizer genes in Xenopus embryos"Dev.Biol.. 239. 241-256 (2001)
[Publications] A.Nakamura, R.Amikura, K.Hanyu, S.Kobayashi: "Me 31B silences translation of oocyte-loclizing RNAs through the formation of cytoplasmic RNP complex during Drosophila oogenesis"Development. 128. 3233-3242 (2001)
[Publications] Hiiratani, I., Mochizuki, T., Tochimoto, N., Taira, M.: "Functional Domains of the LIM Homeodomain Protein Xlim-1 Involved in Negative Regulation, Transactivation, a dn Axis Formation in Xenopus Embryos"Dev.Biol.. 229. 456-467 (2001)
[Publications] Hikasa, H., Taira, M.: "A study of Xlim 1 function in the Spemann-Mangold Organizer"Int.J.Dev.Biol.. 45. 209-218 (2001)
[Publications] Hikasa, H., Taira, M.: "A Xenopus homolog of a human p53-activated gene, PA26, is specifically Expressed in the notochord"Mech.Dev.. 100. 309-312 (2001)
[Publications] Makabe, K.W., Kawashima, T., Kawashima, S., et al.: "Maternal genetic information stored in fertilized eggs of the ascidian, Halocynthia Roretzi"Biology of Ascidian. 0. 165-177 (2001)
[Publications] Darras, S., Nishida, H.: "The BMP/CHORDIN antagonism controls sensory pigment cell specification and Differentiation in the ascidian embryo"Dev.Biol.. 236. 271-288 (2001)
[Publications] A.Nakamura, R.Amikura, K.Hanyu, S.Kobayashi: "Me 31B silences translation of oocyte-loclizing RNAs through the formation of cytoplasmic RNP complex during Drosophila oogenesis"Development. 128. 3233-3242 (2001)
[Publications] Kobayashi, K., Nishida, H.: "Nuclear plasticity and timing mechanisms of the initiation of alkaline phosphatase expression in cytoplasm-transferred blastomeres of ascidians"Dev.Biol.. 234. 510-520 (2001)
[Publications] Kim, G.J., Nishida, H.: "Role of FGF and MEK signaling pathway in the ascidian embryo"Dev.Growth Differ.. 43. 521-533 (2001)
[Publications] Minokawa, T., Yagi, K., Makabe, K.W., Nishida, H.: "Binary specification of nerve cord and notochord cell fates in ascidian embryos"Development. 128. 2007-2017 (2001)
[Publications] Makabe, K.W., Kawashima, T., Kawashima, S., et al.: "Large-scale cDNA analysis of the maternal genetic information in the egg of the ascidian, Halocynthia roretzi, for the gene expression catalog during development"Development. 128. 2555-2567 (2001)
[Publications] Darras, S., Nishida, H.: "The BMP signaling pathway is required together with the FGF pathway for Notochord induction in the ascidian embryos"Development. 128. 2629-2638 (2001)
[Publications] Nishida, H., Sawada, K.: "Binary specification of nerve cord and notochord cell fates in ascidian embryos"Development. 128. 2007-2017 (2001)
[Publications] Kobayashi, K., Nishida, H.: "Macho-1 encodes localized mRNA in ascidian eggs that specifies muscle fate During embryoegnesis"Nature. 409. 724-729 (2001)
[Publications] Ogasawara, M., Minokawa, T., Sasakura, Y., Nishida, H., Makabe, K.W.: "A large-scale whole-mount in situ hybridization system : rapid one-tube preparation of DIG-labeled RNA probes and high through put hybridization using 96-well silent screen plates"Zoological Science. 18. 187-193 (2001)

2001 Fiscal Year Annual Research Report

初期発生制御システムの包括的解析

Principal Investigator

上野 直人 岡崎国立共同研究機構, 基礎生物学研究所, 教授 (40221105)

Research Products

[Publications] Kurata, T., Nakabayashi, J., Yamamoto, T.S., Mochii, M., Ueno, N: "Visualization of endogenous BMP signaling during Xenopus development"Differentiation. 67. 33-40 (2001)

[Publications] Ishizuya, A., Ueda, S., Amano, T., Shimizu, K., Suzuki, K., Ueno, N., Yoshizato, K.: "Thyroid-hormone-dependent and fibroblast-specific expression of BMP-4 correlates With adult epithelial development during amphibian intestinal remodeling"Cell Tissues Res.. 303. 187-195 (2001)

[Publications] Morita, K., Shimizu, M., Shibuya, H., Ueno, N.: "A Daf-1-binding protein BRA-1 ia a negative regulator of Daf-7 TGF-b signaling"Proc.Natl.Acad.Sci.USA. 98. 6284-6288 (2001)

[Publications] Hanazawa, M., Mochii, M., Ueno, N., Kohara, Y., Iino, Y.: "Use of cDNA subtraction and RNA interference screens in combination reveals Genes required for germ-line development in Caenohabditis elegans"Proc.Natl.Acad.Sci.USA. 98. 8686-8691 (2001)

[Publications] Yamamoto, T.S., Takagi, C., Hyodo, A., Ueno, N.: "Suppression of head formation by Xmsx-1 through the inhibition of intracellular nodal signaling"Development. 128. 2769-2779 (2001)

[Publications] Sugawara, K., Morita, K., Ueno, N., Shibuya, H.: "BIP, a BRAM-interacting protein involved in TGF-beta signaling, regulates body length in Caenorhabditis elegans"Gene Cells. 6. 566-606 (2001)

[Publications] Yoshida, S., Morita, K., Mochii, M., Ueno, N.: "Hypodermal expression of Caenorhabditis elegans TGF-b type I receptor SMA-6 is essential for the growth and maintenance of body length"Dev.Biol.. 240. 32-45 (2001)

[Publications] Ohkawwara, B., Iemura, S.-I., ten Dijke, P., Ueno, N.: "Action range of BMP is defined by its N-terminal basic amino acid core"Curr.Biol.. 12. 205-209 (2002)

[Publications] H.Sano, M.Mukai, S.Kobayashi: "Maternal Nanos and Pumilio regulate zygotic vasa expression autonomously in the germline progenitors of Drosophila"Develoop.Growth & Differ.. 43. 545-552 (2001)

[Publications] A.Nakamura, R.Amikura, K.Hanyu, S.Kobayashi: "Me 31B silences translation of oocyte-loclizing RNAs through the formation of cytoplasmic RNP complex during Drosophila oogenesis"Development. 128. 3233-3242 (2001)

[Publications] M.Kashikawa, R.Amikura, S.Kobayashi: "Mitochondrial small rribosomal RNA is a component of germinal granules in Xenopus embryos"Mech.Dev.. 101. 71-77 (2001)

[Publications] R.Amikura, K.Hanyu, M.Kashikawa, S.Kobayashi: "Tudor protein is essential for the localization of mmitochondrial ribosomal RNAs in Polar granules in germ plasm of Drosophila embryos"Mech.Dev.. 107. 97-104 (2001)

[Publications] R.Amikura, M.Kashikawa, A.Nakamura, S.Kobayashi: "Presence of Mitochondrial-type ribosomes outside mitochondria in germ plasm of Drosophila embryos"Proc.Natl.Acad.Sci.USA. 98. 9133-9138 (2001)

[Publications] Shinga, J., Itoh, M., Shiokawa, K., Taira, S., Taira, M.: "Early patterning of the prospective midbrain-hindbrain boundary by the HES-Related gene XHR 1 in Xenopus embryo"Mech.Dev.. 109. 225-239 (2001)

[Publications] Shibata, M., Itoh, M., Ohmori, S.-Y., Shinga, J., Taira, M.: "Systematic screening and expression analysis of the head organizer genes in Xenopus embryos"Dev.Biol.. 239. 241-256 (2001)

[Publications] A.Nakamura, R.Amikura, K.Hanyu, S.Kobayashi: "Me 31B silences translation of oocyte-loclizing RNAs through the formation of cytoplasmic RNP complex during Drosophila oogenesis"Development. 128. 3233-3242 (2001)

[Publications] Hiiratani, I., Mochizuki, T., Tochimoto, N., Taira, M.: "Functional Domains of the LIM Homeodomain Protein Xlim-1 Involved in Negative Regulation, Transactivation, a dn Axis Formation in Xenopus Embryos"Dev.Biol.. 229. 456-467 (2001)

[Publications] Hikasa, H., Taira, M.: "A study of Xlim 1 function in the Spemann-Mangold Organizer"Int.J.Dev.Biol.. 45. 209-218 (2001)

[Publications] Hikasa, H., Taira, M.: "A Xenopus homolog of a human p53-activated gene, PA26, is specifically Expressed in the notochord"Mech.Dev.. 100. 309-312 (2001)

[Publications] Makabe, K.W., Kawashima, T., Kawashima, S., et al.: "Maternal genetic information stored in fertilized eggs of the ascidian, Halocynthia Roretzi"Biology of Ascidian. 0. 165-177 (2001)

[Publications] Darras, S., Nishida, H.: "The BMP/CHORDIN antagonism controls sensory pigment cell specification and Differentiation in the ascidian embryo"Dev.Biol.. 236. 271-288 (2001)

[Publications] A.Nakamura, R.Amikura, K.Hanyu, S.Kobayashi: "Me 31B silences translation of oocyte-loclizing RNAs through the formation of cytoplasmic RNP complex during Drosophila oogenesis"Development. 128. 3233-3242 (2001)

[Publications] Kobayashi, K., Nishida, H.: "Nuclear plasticity and timing mechanisms of the initiation of alkaline phosphatase expression in cytoplasm-transferred blastomeres of ascidians"Dev.Biol.. 234. 510-520 (2001)

[Publications] Kim, G.J., Nishida, H.: "Role of FGF and MEK signaling pathway in the ascidian embryo"Dev.Growth Differ.. 43. 521-533 (2001)

[Publications] Minokawa, T., Yagi, K., Makabe, K.W., Nishida, H.: "Binary specification of nerve cord and notochord cell fates in ascidian embryos"Development. 128. 2007-2017 (2001)

[Publications] Makabe, K.W., Kawashima, T., Kawashima, S., et al.: "Large-scale cDNA analysis of the maternal genetic information in the egg of the ascidian, Halocynthia roretzi, for the gene expression catalog during development"Development. 128. 2555-2567 (2001)

[Publications] Darras, S., Nishida, H.: "The BMP signaling pathway is required together with the FGF pathway for Notochord induction in the ascidian embryos"Development. 128. 2629-2638 (2001)

[Publications] Nishida, H., Sawada, K.: "Binary specification of nerve cord and notochord cell fates in ascidian embryos"Development. 128. 2007-2017 (2001)

[Publications] Kobayashi, K., Nishida, H.: "Macho-1 encodes localized mRNA in ascidian eggs that specifies muscle fate During embryoegnesis"Nature. 409. 724-729 (2001)

上野直人岡崎国立共同研究機構, 基礎生物学研究所, 教授 (40221105)