2003 Fiscal Year Annual Research Report

RNA情報発現系の時空間ネットワーク

Research Project

Project/Area Number	13051101
Research Institution	The University of Tokyo
Principal Investigator	中村義一東京大学, 医科学研究所, 教授 (40114590)
Co-Investigator(Kenkyū-buntansha)	谷口維紹東京大学, 大学院・医学系研究科, 教授 (50133616) 野本明男東京大学, 大学院・医学系研究科, 教授 (70112670) 堀田凱東京大学, 国立遺伝学研究所, 所長(研究職) (30010036) 坂本博神戸大学, 理学部, 教授 (00187048) 渡辺公綱東京大学, 大学院・新領域創成科学研究科, 教授 (00134502)
Keywords	RNA / ネットワーク / RNA蛋白質複合体 / 高次複合機能 / 分子擬態 / ポストゲノム / 転写後調節 / 翻訳調節
Research Abstract	本領域は総括班と4つの計画研究班及び公募研究から構成され、緊密な連絡の下に領域全体および各班の研究目標を達成するように推進された。各研究班の研究成果の概要は以下の通りである。 (総括班)1)日本RNA学会と共催で国際シンポジウム「The New Frontier of RNA Science」を開催した(平成15年11月24-27日、於京都国際会議場)。国外招待講演者25名を含む計380名の参加者のもとに学術的、教育的、国際交流的に実り豊かな第一級の国際会議となった。2)計画研究項目の内容に関して最新の調査点検議論を実施した。3)合同班会議を平成16年2月2〜4日に小樽市マリンヒルホテル小樽において開催した。4)第4回総括班会議を平成15年11月23日(於京都市)に開催した。5)RNA Network Newsletter第3、4号を発行し、メディアを含む関係方面へ各2000部配送し、領域研究の啓蒙を行った。 (A01班・RNPマシーン)酵母2型解離因子eRF3の結晶構造の解明、ペプチド解離の触媒塩基の発見、再生因子RRFのEF-Gの転移活性非依存性等の証明等、リボソーム研究を推進した。又、古細菌と真核生物の各種アミノアシルtRNA合成酵素の単体や共結晶の構造解明tRNA修飾酵素の結晶構造と反応機作の解明、ミトコンドリアtRNA修飾異常による疾患発症の分子基盤解明を行った。 (A02班・RNA制御スイッチ)翻訳伸長と解離のスイッチを担うリボソームRNA塩基の同定と活性中心変異体の系統的な分離解析により動的作動機構に関する知見の集積、植物における翻訳と共役したmRNA分解制御、精巣特異的細胞質ポリ(A)ポリメラーゼの欠損マウスの作製と解析、RNA関与MAPK信号伝達系のフィードバック制御の発見等、RNA制御スイッチに関する重要な知見をえた。 (A03班・動くRNA)神経特異的RNA結合蛋白質Huの線虫ホモログによる老廃物排出器官形成制御の発見、スプライシング調節RNA結合タンパク質Y14による線虫の生殖細胞の性分化制御の発見等、スプライシングやmRNA核外輸送のカップリング等に関する分子基盤が明らかにされた。 (A04班・高次複合系RNA動態)RNA干渉(RNAi)は真核生物の遺伝子制御の新パラダイムであるが、脆弱X症候群原因遺伝子(FMR1)のショウジョウバエ相同体の解析により「RNAi関連分子装置の異常による疾患」というヒト分子遺伝学における全く新しい領域を開いた。

Research Products

(40 results)

All Other

All Publications (40 results)

[Publications] Crist, C.: "[PHI^+], a novel Sup35-prion variant propagated with non-Gln/Asn oligopeptide repeats in the absence of the chaperone protein Hsp104."Genes Cells. 8. 603-618 (2003)
[Publications] Oguro, A.: "RNA aptamers to initiation factor 4A helicase hinder cap-dependent translation by blocking ATP hydrolysis."RNA. 9. 394-407 (2003)
[Publications] Polacek, N.: "The critical role of the universally conserved A2602 of 23S ribosomal RNA in the release of the nascent peptide during translation termination."Mol.Cell. 11. 103-112 (2003)
[Publications] Nakamura, Y.: "Making sense of mimic in translation termination."Trends Biochem.Sci.. 28. 99-105 (2003)
[Publications] Blake, B.K.: "Backbone ^1H, ^<13>C, and ^<15>N assignments of the ribosome recycling factor from Thermus thermophilus."J.Biomol.NMR. 24. 81-82 (2002)
[Publications] Laursen, B.S.: "Characterization of mutations in the GTP-binding domain of IF2 resulting in cold-sensitive growth of Escherichia coli."J.Mol.Biol.. 326. 543-551 (2003)
[Publications] Karamysheva, Z.N.: "Antizyme frameshifting as a functional probe of eukaryotic translational termination."Nuci.Acids Res.. 31. 5949-5956 (2003)
[Publications] Hara, H.: "Prion domain interaction responsible for species discrimination in yeast [PSI^+] transmission."Genes Cells. 8. 925-939 (2003)
[Publications] Kong, C.: "Crystal Structure and Functional Analysis of the Eukaryotic Class II Release Factor eRF3 from S. Pombe"Mol.Cell. (In press). (2004)
[Publications] Fujiwara, T.: "Ribosome recycling factor disassembles the posttermination ribosomal complex independent of the ribosomal translocase activity of elongation factor G."Mol.Microbiol.. (In press). (2004)
[Publications] Murai N.: "Identification of nuclear export signals in antizyme-1."J Biol Chem.. 278(45). 44791-44798 (2003)
[Publications] Yanaihara N.: "A commonly deleted region in ovarian cancer on chromosome 19p13.3, not including the QAZ1 gene."IntJ Oncol.. 23(3). 567-575 (2003)
[Publications] Fujita, M.: ")The role of the ELAV homologue EXC-7 in the development of the Caenorhabditis elegans excretory canals."Dev.Biol.. 256. 290-301 (2003)
[Publications] Kawano, T.: "Ce-Y 14 and MAG-1, components of the exon-exon junction complex, are required for embryogenesis and germline sexual switching in Caenorhabditis eleigans."Mech.Dev.. 121. 27-35 (2004)
[Publications] Saijou, E.: "RBD-1, a nucleolar RNA-binding protein, is essential for Caenorhabditis elegans early development through 18S ribosomal RNA processing"Nucleic Acids Res.. 32. 1028-1036 (2004)
[Publications] Okamura, K.: "Distinct roles for Argonaute proteins in the small RNA-directed silencing mechanism"Science. (In press). (2004)
[Publications] Siomi, H.: "RNA Interference : A New Mechanism by Which FMRP Acts in the Normal Brain? - What can Drosophila teach us?"MRDDR review. 10. 68-74 (2004)
[Publications] Taura, T.: "The molecular mechanisms of mRNA export. In Nuclear transport of RNA and proteins."Landes Bioscience. (In press). (2004)
[Publications] Nakai, Y.: "Yeast Nfslp is involved in thio-modification of both mitochondrial and cytoplasmic tRNAs."J.Biol.Chem.. (In press). (2004)
[Publications] Kurata, S.: "Quick two-step RNA ligation employing periodate oxidation."Nucleic Acids Res.. 31. e145 (2003)
[Publications] Soma, A.: "An RNA-modifying enzyme that governs both the codon and amino acid specificities of isoleucine tRNA."Mol Cell. 12. 689-698 (2003)
[Publications] Kamiya, N.: "S-peptide as a potent peptidyl linker for protein crosslinking by microbial transglutaminase from Streptomvces mobaraensis."Bioconjugate Chemistry. 14. 351-357 (2003)
[Publications] Kaneko, T.: "Wobble modification differences and subcellular localization of tRNAs in Leishmania tarentolae : implication for tRNA sorting mechanism."EMBO J.. 22. 657-667 (2003)
[Publications] Kaminishi, T.: "Crystallization and preliminary X-ray diffraction analysis of ribosomal protein L11 methyltransferase from Thermus thermophilus HB8"Acta Crystallogr D Biol Crystallogr. 59(7). 930-932 (2003)
[Publications] Ishitani, R.: "Alternative tertiary structure of tRNA for recognition by a posttranscrintional modification enzyme"Cell. 113(3). 383-394 (2003)
[Publications] Ishii, R.: "Crystal structure of the tRNA processing enzyme RNase PH from Aauifex aeolicus"J.Biol.Chem.. 278(34). 32397-32404 (2003)
[Publications] Kobayashi, T.: "Structural basis for orthogonal tRNA specificities of tyrosyl-tRNA synthetases for genetic code expansion"Nat Struct Biol. 10(6). 425-432 (2003)
[Publications] Kato, M.: "Crystal structure of the 2'-5' RNA ligase from Thermus thermophilus HB8"J Mol Biol. 329(5). 903-911 (2003)
[Publications] Polycarpo, C.: "Activation of the Pyrrolysine Suppressor tRNA Requires Formation of a Ternary Complex with Class I and Class II Lysyl-tRNA Synthetases"Mol Cell.. 12(2). 287-294 (2003)
[Publications] Okabe, M.: "Divergent evolutions of trinucleotide polymerization revealed by an archaeal CCA-adding enzyme structure"EMBO J. 22(21). 5918-5927 (2003)
[Publications] Fukunaga, R.: "Crystal structures of the CP1 domain from thermus thermophilus isoleucvl-tRNA synthetase and its complex with L-valine"J.Biol.Chem.. 279(9). 8396-8402 (2003)
[Publications] Sengoku, T.: "Crystallization and preliminary X-ray analysis of the helicase domains of Vasa complexed with RNA and an ATP analogue"Acta Crystallogr D Biol Crystallogr.. 60(2). 320-322 (2004)
[Publications] Kise, Y: "A short peptide insertion crucial for angiostatic activity of human tryptophanyl-tRNA svnthetase"Nat.Struct.Mol.Biol.. 11(2). 149-156 (2004)
[Publications] Temiakov, D.: "Structural Basis for Substrate Selection by T7 RNA Polymerase"Cell. 116(3). 381-391 (2004)
[Publications] Hirao I.: "In vitro selection of RNA aptamers that bind to colicin E3 and structurally resemble the decoding site of 16S ribosomal RNA"Biochem. (In press).
[Publications] Yamamoto, Y.: "SsrA-mediated trans-translation plays a role in mRNA quality control by facilitating degradation of truncated mRNAs."RNA. 9. 408-418 (2003)
[Publications] Morita, T.: "Accumulation of Glucose-6-phosphate or Fructose-6-phosphate Is Responsible for Destabilization of Glucose Transporter mRNA in Escherichia coli."J. Biol. Chem.. 15608-15614 (2003)
[Publications] EI-Kazzaz, W.: "Metabolic block at early stages of glycolytic pathway activates the Rcs phosphorelay system via increased synthesis of dTDP-glucose in Escherichia coli."Mel. Microbiol. 51. 1114-1128 (2004)
[Publications] Sunohara, T.: "Nascent peptide-mediated ribosome stalling at a stop codon induces mRNA cl eavage resulting in nonstop mRNA that is recognized by tmRNA"RNA. 10. 378-386 (2004)
[Publications] Sunohara, T.: "Ribosome stalling during translation elongation induces cleavage of mRNA being translated in Escherichia coli."J. Bid. Chem. (In press). (2004)

2003 Fiscal Year Annual Research Report

RNA情報発現系の時空間ネットワーク

Principal Investigator

中村 義一 東京大学, 医科学研究所, 教授 (40114590)

Research Products

[Publications] Crist, C.: "[PHI^+], a novel Sup35-prion variant propagated with non-Gln/Asn oligopeptide repeats in the absence of the chaperone protein Hsp104."Genes Cells. 8. 603-618 (2003)

[Publications] Oguro, A.: "RNA aptamers to initiation factor 4A helicase hinder cap-dependent translation by blocking ATP hydrolysis."RNA. 9. 394-407 (2003)

[Publications] Polacek, N.: "The critical role of the universally conserved A2602 of 23S ribosomal RNA in the release of the nascent peptide during translation termination."Mol.Cell. 11. 103-112 (2003)

[Publications] Nakamura, Y.: "Making sense of mimic in translation termination."Trends Biochem.Sci.. 28. 99-105 (2003)

[Publications] Blake, B.K.: "Backbone ^1H, ^<13>C, and ^<15>N assignments of the ribosome recycling factor from Thermus thermophilus."J.Biomol.NMR. 24. 81-82 (2002)

[Publications] Laursen, B.S.: "Characterization of mutations in the GTP-binding domain of IF2 resulting in cold-sensitive growth of Escherichia coli."J.Mol.Biol.. 326. 543-551 (2003)

[Publications] Karamysheva, Z.N.: "Antizyme frameshifting as a functional probe of eukaryotic translational termination."Nuci.Acids Res.. 31. 5949-5956 (2003)

[Publications] Hara, H.: "Prion domain interaction responsible for species discrimination in yeast [PSI^+] transmission."Genes Cells. 8. 925-939 (2003)

[Publications] Kong, C.: "Crystal Structure and Functional Analysis of the Eukaryotic Class II Release Factor eRF3 from S. Pombe"Mol.Cell. (In press). (2004)

[Publications] Fujiwara, T.: "Ribosome recycling factor disassembles the posttermination ribosomal complex independent of the ribosomal translocase activity of elongation factor G."Mol.Microbiol.. (In press). (2004)

[Publications] Murai N.: "Identification of nuclear export signals in antizyme-1."J Biol Chem.. 278(45). 44791-44798 (2003)

[Publications] Yanaihara N.: "A commonly deleted region in ovarian cancer on chromosome 19p13.3, not including the QAZ1 gene."IntJ Oncol.. 23(3). 567-575 (2003)

[Publications] Fujita, M.: ")The role of the ELAV homologue EXC-7 in the development of the Caenorhabditis elegans excretory canals."Dev.Biol.. 256. 290-301 (2003)

[Publications] Kawano, T.: "Ce-Y 14 and MAG-1, components of the exon-exon junction complex, are required for embryogenesis and germline sexual switching in Caenorhabditis eleigans."Mech.Dev.. 121. 27-35 (2004)

[Publications] Saijou, E.: "RBD-1, a nucleolar RNA-binding protein, is essential for Caenorhabditis elegans early development through 18S ribosomal RNA processing"Nucleic Acids Res.. 32. 1028-1036 (2004)

[Publications] Okamura, K.: "Distinct roles for Argonaute proteins in the small RNA-directed silencing mechanism"Science. (In press). (2004)

[Publications] Siomi, H.: "RNA Interference : A New Mechanism by Which FMRP Acts in the Normal Brain? - What can Drosophila teach us?"MRDDR review. 10. 68-74 (2004)

[Publications] Taura, T.: "The molecular mechanisms of mRNA export. In Nuclear transport of RNA and proteins."Landes Bioscience. (In press). (2004)

[Publications] Nakai, Y.: "Yeast Nfslp is involved in thio-modification of both mitochondrial and cytoplasmic tRNAs."J.Biol.Chem.. (In press). (2004)

[Publications] Kurata, S.: "Quick two-step RNA ligation employing periodate oxidation."Nucleic Acids Res.. 31. e145 (2003)

[Publications] Soma, A.: "An RNA-modifying enzyme that governs both the codon and amino acid specificities of isoleucine tRNA."Mol Cell. 12. 689-698 (2003)

[Publications] Kamiya, N.: "S-peptide as a potent peptidyl linker for protein crosslinking by microbial transglutaminase from Streptomvces mobaraensis."Bioconjugate Chemistry. 14. 351-357 (2003)

[Publications] Kaneko, T.: "Wobble modification differences and subcellular localization of tRNAs in Leishmania tarentolae : implication for tRNA sorting mechanism."EMBO J.. 22. 657-667 (2003)

[Publications] Kaminishi, T.: "Crystallization and preliminary X-ray diffraction analysis of ribosomal protein L11 methyltransferase from Thermus thermophilus HB8"Acta Crystallogr D Biol Crystallogr. 59(7). 930-932 (2003)

[Publications] Ishitani, R.: "Alternative tertiary structure of tRNA for recognition by a posttranscrintional modification enzyme"Cell. 113(3). 383-394 (2003)

[Publications] Ishii, R.: "Crystal structure of the tRNA processing enzyme RNase PH from Aauifex aeolicus"J.Biol.Chem.. 278(34). 32397-32404 (2003)

[Publications] Kobayashi, T.: "Structural basis for orthogonal tRNA specificities of tyrosyl-tRNA synthetases for genetic code expansion"Nat Struct Biol. 10(6). 425-432 (2003)

[Publications] Kato, M.: "Crystal structure of the 2'-5' RNA ligase from Thermus thermophilus HB8"J Mol Biol. 329(5). 903-911 (2003)

[Publications] Polycarpo, C.: "Activation of the Pyrrolysine Suppressor tRNA Requires Formation of a Ternary Complex with Class I and Class II Lysyl-tRNA Synthetases"Mol Cell.. 12(2). 287-294 (2003)

[Publications] Okabe, M.: "Divergent evolutions of trinucleotide polymerization revealed by an archaeal CCA-adding enzyme structure"EMBO J. 22(21). 5918-5927 (2003)

[Publications] Fukunaga, R.: "Crystal structures of the CP1 domain from thermus thermophilus isoleucvl-tRNA synthetase and its complex with L-valine"J.Biol.Chem.. 279(9). 8396-8402 (2003)

[Publications] Sengoku, T.: "Crystallization and preliminary X-ray analysis of the helicase domains of Vasa complexed with RNA and an ATP analogue"Acta Crystallogr D Biol Crystallogr.. 60(2). 320-322 (2004)

[Publications] Kise, Y: "A short peptide insertion crucial for angiostatic activity of human tryptophanyl-tRNA svnthetase"Nat.Struct.Mol.Biol.. 11(2). 149-156 (2004)

[Publications] Temiakov, D.: "Structural Basis for Substrate Selection by T7 RNA Polymerase"Cell. 116(3). 381-391 (2004)

[Publications] Hirao I.: "In vitro selection of RNA aptamers that bind to colicin E3 and structurally resemble the decoding site of 16S ribosomal RNA"Biochem. (In press).

[Publications] Yamamoto, Y.: "SsrA-mediated trans-translation plays a role in mRNA quality control by facilitating degradation of truncated mRNAs."RNA. 9. 408-418 (2003)

[Publications] Morita, T.: "Accumulation of Glucose-6-phosphate or Fructose-6-phosphate Is Responsible for Destabilization of Glucose Transporter mRNA in Escherichia coli."J. Biol. Chem.. 15608-15614 (2003)

[Publications] EI-Kazzaz, W.: "Metabolic block at early stages of glycolytic pathway activates the Rcs phosphorelay system via increased synthesis of dTDP-glucose in Escherichia coli."Mel. Microbiol. 51. 1114-1128 (2004)

[Publications] Sunohara, T.: "Nascent peptide-mediated ribosome stalling at a stop codon induces mRNA cl eavage resulting in nonstop mRNA that is recognized by tmRNA"RNA. 10. 378-386 (2004)

[Publications] Sunohara, T.: "Ribosome stalling during translation elongation induces cleavage of mRNA being translated in Escherichia coli."J. Bid. Chem. (In press). (2004)

中村義一東京大学, 医科学研究所, 教授 (40114590)