2001 Fiscal Year Annual Research Report

アクアポリンと唾液分泌

Research Project

Project/Area Number	13137207
Research Category	Grant-in-Aid for Scientific Research on Priority Areas (B)
Research Institution	The University of Tokushima
Principal Investigator	石田甫徳島大学, 歯学部, 教授 (70028364)
Co-Investigator(Kenkyū-buntansha)	飯田博一徳島大学, 歯学部, 助手 (10335797) 石川康子徳島大学, 歯学部, 助教授 (40144985)
Keywords	水チャネル / アクアポリン-5 / アクアポリン-8 / 唾液腺 / M3ムスカリン受容体 / 細胞内カルシウム濃度 / NO合成酵素 / cGMP
Research Abstract	耳下腺管腔膜に局在する水輸送体AQP5増量を誘導する細胞内情報伝達系の検索を、本年度は主に行い、下記のような成果を得た。 8週齢ラットより耳下腺切片を調製し、acethycholine(ACh)、epinephrine(Epi)、cis-2-methylspiro-(1,3-oxathiolane-5,3')-quinuclidine(SNI-2011)やpilocarpineをはじめ諸種薬物とKrebs Ringer Tris(KRT)液(pH7.4)中で37℃にて15秒から30分間加温振盪した後、LongbottomらやVayroらの方法を応用して基底膜,管腔膜,細胞内小胞膜等に細胞分画する。SDS-PAGEに供した後、ニトロセルローズ膜に転写し、C末側アミノ酸配列を用いて調製した抗AQP5抗体を使ってウェスタンブロッティングを行い、デンシトメーターにて解析した。 1.AChやpilocarpineによる耳下腺管腔膜におけるAQP5の増量はGF109203Xによって阻止されず、U73122、Carboxy-PTIOによって阻止された。 2.dbcGMP, SIN-1,SNAP, Atriopeptin Sodium azide単独によってもAQP5の増量は誘導され,KT5823によって阻止された。 3.AChやpilocarpineによる耳下腺管腔膜におけるAQP5の増量はKN-93,L-Nil, ODQ, ML-9によって阻止された。 4.AChやEpiによる耳下腺管腔膜におけるAQP5の増量はTubulozole-C, Cytochalasin Dによって阻止された。 5.細胞外Ca^<2+>の存在下AChによる耳下腺管腔膜におけるAQP5の増量は3.8倍、細胞外Ca^<2+>の非存在下AChによる耳下腺管腔膜におけるAQP5の増量は2倍であり、また、dbcGMP,によってるAQP5の増量はBAPTA-AMによって阻止された。以上のことから、耳下腺管腔膜のAQP5の機能の発現には神経系が関与しており、細胞内情報伝達系として細胞内Ca^<2+>上昇によって誘導されるNitric oxide synthetase/Soluble guanyl cyclase系の関与が示唆された。

Research Products

(17 results)

All Other

All Publications (17 results)

[Publications] Yasuko Ishikawa: "Activation of endogenous nitric oxide synthase coupled with methacholine induced exocytosis in rat parotid acinar cells"J.Pharmacol. Exp. Ther.. Vol.301 No.1. 1-9 (2002)
[Publications] Yasuko Ishikawa: "Regulation of Gi2α phoshporylation controls isoprenaline-induced super-sensitivity and desensitization of amylase secretion in rat parotid glands"9^<th> International Catecholamine Symposium. 44 (2001)
[Publications] Yasuko Ishikawa: "Neurotransmitters regulate amount of AQP5 in apical membrane of parotid"Journal Dental Research. Vol.80. 600 (2001)
[Publications] Hajime Ishida: "Phosphorylation/dephosphorylation of Gi regulates desensitization/supersensitivity in response to β2-adrenergic agonist"Journal Dental Research. Vol.80. 676 (2001)
[Publications] Noriko Inoue: "SNI-2011-induced persistent increase in the amount of AQP5 in APM"Journal Dental Research. Vol.80. 676 (2001)
[Publications] 石川康子: "耳下腺のAQP5とAQP8の神経性調節"歯科基礎医学会雑誌. Vol.43,No.5. 551 (2001)
[Publications] 石田甫: "コリン作動薬による耳下腺腺房細胞におけるNOとcGMPの生成とアミラーゼ分泌"歯科基礎医学会雑誌. Vol.43,No.5. 588 (2001)
[Publications] 井上紀子: "糖尿病と唾液腺分泌磯能の変化"歯科基礎医学会雑誌. Vol.43,No.5. 588 (2001)
[Publications] 石川康子: "SNI-2011による持続性唾液分泌と水チャネルAQP5の連関性"日本唾液腺学会誌. 42. 39-42 (2001)
[Publications] Yasuko Ishikawa: "The activation of NO/cGMP signal transduction induces the increase in AQP5 levels in the apical plasma membrane in rat parotid glands"Japanese Journal of Pharmacology. Vol.81,Suppl. 221 (2002)
[Publications] Hirokazu Iida: "Activation of endogenous NOS coupled with methacholine(MCh)-induced amylase secretion exocytosis in rat parotid acinar cells"Japanese Journal of Pharmacology. Vol.81,Suppl. 174 (2002)
[Publications] Hajime Ishida: "Regulation of phosphorylation status of Gi2α couples with β2-adrenergic agonist-induced desensitization and supersensitivity"XIVth International Congress of Pharmacology. (in press). (2002)
[Publications] Yasuko Ishikawa: "Activation of endogenous nitric oxide synthase coupled with methacholine-induced exocytosis in rat parotid acinar cells"XIVth International Congress of Pharmacology. (in press). (2002)
[Publications] Yasuko Ishikawa: "The ACh-and pilocarpine-induced increase in AQP5 levels in the APM in rat parotid acinar cells is coupled with activation of..."XIVth International Congress of Pharmacology. (in press). (2002)
[Publications] 石田甫: "「顎関節症入門」薬物療法編著;森本俊文, 松矢篤三, 野首孝祠, 小林義典"医歯薬出版株式会社. 249 (2001)
[Publications] Yasuko Ishikawa: "「Molecular biology and physiology of water and solute transport」 Edited by Stefan"Kluwer Academic/Plenum Publishers, London, UK.. 451 (2001)
[Publications] Hajime Ishida: "「Histamine Research in the New Millennium」 Edited by Takehiko Watanabe, Henk Timmerman and Kazuhiko Yanai"Elsevier Science Publishing Co, Amsterdam, The Netherlands. 521 (2001)

2001 Fiscal Year Annual Research Report

アクアポリンと唾液分泌

Principal Investigator

石田 甫 徳島大学, 歯学部, 教授 (70028364)

Research Products

[Publications] Yasuko Ishikawa: "Activation of endogenous nitric oxide synthase coupled with methacholine induced exocytosis in rat parotid acinar cells"J.Pharmacol. Exp. Ther.. Vol.301 No.1. 1-9 (2002)

[Publications] Yasuko Ishikawa: "Regulation of Gi2α phoshporylation controls isoprenaline-induced super-sensitivity and desensitization of amylase secretion in rat parotid glands"9^<th> International Catecholamine Symposium. 44 (2001)

[Publications] Yasuko Ishikawa: "Neurotransmitters regulate amount of AQP5 in apical membrane of parotid"Journal Dental Research. Vol.80. 600 (2001)

[Publications] Hajime Ishida: "Phosphorylation/dephosphorylation of Gi regulates desensitization/supersensitivity in response to β2-adrenergic agonist"Journal Dental Research. Vol.80. 676 (2001)

[Publications] Noriko Inoue: "SNI-2011-induced persistent increase in the amount of AQP5 in APM"Journal Dental Research. Vol.80. 676 (2001)

[Publications] 石川康子: "耳下腺のAQP5とAQP8の神経性調節"歯科基礎医学会雑誌. Vol.43,No.5. 551 (2001)

[Publications] 石田甫: "コリン作動薬による耳下腺腺房細胞におけるNOとcGMPの生成とアミラーゼ分泌"歯科基礎医学会雑誌. Vol.43,No.5. 588 (2001)

[Publications] 井上紀子: "糖尿病と唾液腺分泌磯能の変化"歯科基礎医学会雑誌. Vol.43,No.5. 588 (2001)

[Publications] 石川康子: "SNI-2011による持続性唾液分泌と水チャネルAQP5の連関性"日本唾液腺学会誌. 42. 39-42 (2001)

[Publications] Yasuko Ishikawa: "The activation of NO/cGMP signal transduction induces the increase in AQP5 levels in the apical plasma membrane in rat parotid glands"Japanese Journal of Pharmacology. Vol.81,Suppl. 221 (2002)

[Publications] Hirokazu Iida: "Activation of endogenous NOS coupled with methacholine(MCh)-induced amylase secretion exocytosis in rat parotid acinar cells"Japanese Journal of Pharmacology. Vol.81,Suppl. 174 (2002)

[Publications] Hajime Ishida: "Regulation of phosphorylation status of Gi2α couples with β2-adrenergic agonist-induced desensitization and supersensitivity"XIVth International Congress of Pharmacology. (in press). (2002)

[Publications] Yasuko Ishikawa: "Activation of endogenous nitric oxide synthase coupled with methacholine-induced exocytosis in rat parotid acinar cells"XIVth International Congress of Pharmacology. (in press). (2002)

[Publications] Yasuko Ishikawa: "The ACh-and pilocarpine-induced increase in AQP5 levels in the APM in rat parotid acinar cells is coupled with activation of..."XIVth International Congress of Pharmacology. (in press). (2002)

[Publications] 石田甫: "「顎関節症入門」薬物療法 編著;森本俊文, 松矢篤三, 野首孝祠, 小林義典"医歯薬出版株式会社. 249 (2001)

[Publications] Yasuko Ishikawa: "「Molecular biology and physiology of water and solute transport」 Edited by Stefan"Kluwer Academic/Plenum Publishers, London, UK.. 451 (2001)

[Publications] Hajime Ishida: "「Histamine Research in the New Millennium」 Edited by Takehiko Watanabe, Henk Timmerman and Kazuhiko Yanai"Elsevier Science Publishing Co, Amsterdam, The Netherlands. 521 (2001)

石田甫徳島大学, 歯学部, 教授 (70028364)

[Publications] 石田甫: "「顎関節症入門」薬物療法編著;森本俊文, 松矢篤三, 野首孝祠, 小林義典"医歯薬出版株式会社. 249 (2001)