2005 Fiscal Year Final Research Report Summary
Molecular mechanism of macrophage activation and intracellular parasitism by intracellular parasitic bacteria
| Project/Area Number |
13226042
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| Research Category |
Grant-in-Aid for Scientific Research on Priority Areas
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| Allocation Type | Single-year Grants |
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| Review Section |
Biological Sciences
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| Research Institution | Kyoto University |
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Principal Investigator |
MITSUYAMA Masao Kyoto University, Graduate School of medicine, Professor, 大学院・医学研究科, 教授 (10117260)
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| Project Period (FY) |
2001 – 2005
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| Keywords | intracellular parasitic bacteria / Listeria monocvtogenes / Mycobacterium tuberculosis / macrophage / cytokine / listeriolysin O |
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| Research Abstract |
(1) Listeriolysin 0 (LLO) is the major virulence factor of Listeria monocytogenas a representative intracellular parasitic bacteria, and exhibits a strong cytokine-inducing activity in addition to its role for the escape of this bacterium by cytolytic activity in the host macrophaes. Our study dearly showed that the cytokine-inducing activity, especially gammainterferon-inducing activity is dependent on the N-terminus while the cytolytic activity depends on the C-terminus
(2) It was shown that all the cytokine-inducing CDC family proteins including LW filbt activate macrophages far IL-12 and IL-18 and. these macrophage-derived cytokines in turn stimulate NK cells for gamma interferon production that is essential for the induction of Thl-dependent protective immunity
(3) There is a significant difference in the cytokine-inducing ability among LLO and allied proteins produced by Listeria seeligeri and Listeria ivanovii. We were able to show that such a difference is highly dependent on the structural difference at the N-terminus domain. A contribution of unique molecule recognizing LLO inside macrophage cytosol was also suggested.
(4) Thl cytokine induction by Mycobacterium tuberculosis requires the stimulation with viable bacteria. It was shown that some growing bacteria-derive factor that depends on bacterial metabolism is required.
(5) In experimental model of allergic condition in mice, LLO and allied protein with Thl cytokine-inducing activity were effective for the alleviation and control of allergic response.
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Research Products
(73 results)
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[Journal Article] Mycobacterium tuberculosis H37Rv is capable of inhibiting necrosis of the infected macrophages via activation of caspase-9.2007
Author(s)
Uchiyama R, Kawamura I, Kawanishi M, Tsuchiya K, Tominaga T, Kaku T, Fukasawa Y, Sakai S, Fujimura T, Nomura T, Mitsuyama M
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Journal Title
Infect. Immun. 75:(in press)
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Limited contribution of Toll-like receptor (TLR) 2 and TLR4 to the host response to a fungal infectious pathogen, Cryptococcus neoformans.2006
Author(s)
Nakamura K, Miyagi K, Koguchi Y, Kinjo Y, Uezu K, Kinjo T, Akamine M, Fujita J, Kawamura I, Mitsuyama M, Adachi Y, Ohno N, Takeda K, Akira S, Miyazato A, Kaku M, Kawakami K.
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Journal Title
FEMS Immunol. Med. Microbiol. 47
Pages: 148-154
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Sustained-release vancomycin sheet may help to prevent prosthetic graft methicillin -resistant Staphylococcus aureus infection.2006
Author(s)
Hirose K, Marui A, Arai Y, Nomura T, Inoue S, Kaneda K, Kamitani T, Fujita M, Mitsuyama M, Tabata Y, Komeda M
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Journal Title
J. Vasc. Surg. 44
Pages: 377-382
Description
「研究成果報告書概要(欧文)」より
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[Book] 結核(分担執筆)2001
Author(s)
光山 正雄
Total Pages
414
Publisher
医薬ジャーナル社
Description
「研究成果報告書概要(和文)」より
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