2005 Fiscal Year Final Research Report Summary
Basic study for the recombinant SERA malaria vaccine development
| Project/Area Number |
13226058
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| Research Category |
Grant-in-Aid for Scientific Research on Priority Areas
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| Allocation Type | Single-year Grants |
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| Review Section |
Biological Sciences
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| Research Institution | Osaka University |
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Principal Investigator |
HORII Toshihiro OSAKA UNIVERSITY, RESEARCH INSTITUTE FOR MICROBIAL DISEASES, PROFESSOR, 微生物病研究所, 教授 (80142305)
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| Co-Investigator(Kenkyū-buntansha) |
ARISUE Nobuko OSAKA UNIVERSITY, RESEARCH INSTITUTE FOR MICROBIAL DISEASES, RESEARCH ASSOCIATE, 微生物病研究所, 助手 (00242339)
TAI Kumiko OSAKA UNIVERSITY, RESEARCH INSTITUTE FOR MICROBIAL DISEASES, TECHNICAL OFFICIAL, 微生物病研究所, 教務職員 (00187907)
ISHIBASHI Masahide THE RESEARCH FOUNDATION FOR MICROBIAL DISEASES OF OSAKA UNIVERSITY, KAN-ON,JI INSTITUTE, DIRECTOR, 観音寺研究所, 所長(研究職) (70029776)
KAWAMOTO Fumihiko OITA UNIVERSITY, INSTITUTE OF SCIENTIFIC RESEARCH, PROFESSOR, 総合科学研究支援センター, 教授 (40115556)
MATSUMOTO Yoshitsugu THE UNIVERSITY OF TOKYO, GRADUATE SCHOOL OF AGRICULTURAL AND LIFE SCIENCES, ASSOCIATE, PROFESSOR, 大学院・農学生命科学研究科, 助教授 (00173922)
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| Project Period (FY) |
2001 – 2005
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| Keywords | Malaria Vaccine / SE36 Protein / P falciparum / clinical research / enidemiological research / P vivax / SERA gene / Sauirrel monke |
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| Research Abstract |
We have been developing an effective malaria vaccine by using a recombinant SE36 protein, N-terminal domain of Serine Repeat Antigen (SERA5) of Plasmodium falcaprum. Cross sectional field studies have been conducted to evaluate the contribution of naturally acquired immunity of SE36 protein in malaria protection and shown that a strong negative correlations between anti-SE36 IgG3 titer and both of the symptoms and blood parasitemia. In addition, we found that sero-conversion rates against SE36 were 50% or less in adults and less than 10% in younger generations in the cohorts. These findings strongly suggest that N-terminal domain of SERA is a hidden protective antigen like as Achilles heel of malaria parasite.
The clinical grade BK-SE36, the purified SE36 protein adsorbed to aluminum hydroxide gel, prepared under GMP conditions was safe and highly immunogenic in Chimpanzees. In addition, the product provided significant protection in Squirrel monkeys after P falciparum challenge infection. The antibody titers in immunized monkeys were boosted by the challenge infection. Safety and Immunogenicity of BK-SE36 were confirmed in Phase I clinical trial in Japan.
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[Journal Article] Crystallization and preliminary crystallographic analysis of orotidine 5'-monophosphate decarboxylase from human malaria parasite Plasmodium falciparum.2006
Author(s)
Krungkrai S.R., Tokuoka K, Kusakari Y, Inoue T, Adachi H, Matsumura H, Takano K, Murakami S., Mori Y, Kai Y, Krungkrai J, Horii T
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Journal Title
Acta Crystallographica Section F 62(Pt6)
Pages: 542-545
Description
「研究成果報告書概要(和文)」より
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[Journal Article] Toll-Like Receptor 9 Mediates Innate Immune Activation by Malaria Pigment Hemozoin.2005
Author(s)
Coban C, Ishii KJ, Kawai T, Hemmi H, Sato S, Uematsu S, Yamamoto M, Takeuchi O, Itagaki S, Kumar N, Horii T, Akira S
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Journal Title
J.Exp.Med. 201.1
Pages: 19-25
Description
「研究成果報告書概要(和文)」より
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[Journal Article] Toll-Like Receptor 9 Mediates Innate Immune Activation by Malaria Pigment Hemozoin.2005
Author(s)
Coban C., Ishii K.J., Kawai T., Hemmi H., Sato S., Uematsu S., Yamamoto M., Takeuchi 0., Itagaki S., Kumar N., Horii T., Akira S.
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Journal Title
J. Exp. Med. 201(1)
Pages: 19-25
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Crystallization and preliminary crystallographic analysis of orotidine 5'-monophosphate decarboxylase from human malaria parasite Plasmodium falciparum.
Author(s)
Krungkrai S.R., Tokuoka K., Kusakari Y., Inoue T., Adachi H., Matsumura H., Takano K., Murakami S., Mori Y., Kai Y., Krungkrai J., Horii T.
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Journal Title
Acta Crystallographica Section F in Press
Description
「研究成果報告書概要(欧文)」より
