| Research Abstract |
1.Endothelial function in physiological and pathological vascular remodeling
From studies concerning atherosclerosis, mechanism of functional angiogenesis, differentiation mechanism of hepatic stem cell, the main results obtained were as follows :
1)Atherosclerosis and angiogenesis : intimal neovascularization related to VEGF-A and -C expression was equivalent to a histopathological hallmark of active atherogenesis, and we propose "inflammation-repair hypothesis" for atherogenesis.
2)Molecular mechanism of functional angiogenesis : FGF-2 hierarchically enhanced the expression of VEGF,HGF,PDGF and others in a hypoxic tissues, leading to the integrated angiogenesis associated with structural and functional interaction between endothelial-pericytes(smooth muscle cells).
3)Differentiation mechanism of hepatic stem cells : FLK-1-positive cells in mouse fetuses possessed the pluripotent differentiation activities into hepatocytes and bile ducts, and blood vessels under HGF+EGF and VEGF+PDGF-BB stimulation, respectively.
2.Biological properties of novel gene transfer vectors and their clinical application
Using recombinant SeV and SIV vectors with several reporter and target genes(FGF-1,-2,-10,VEGF-A,PEDF,sprouty-2,soluble PDGF receptor etc), biological and pathbphysiological characteritics of spatial and time-sequential transfer/expression efficacy of these vectors were analyzed and we are exploiting the novel strategies on gene therapy.
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