2002 Fiscal Year Annual Research Report

自己幹細胞を用いた自己組織再生誘導型バイオ人工臓器

Research Project

Project/Area Number	13358016
Research Institution	Kyoto University
Principal Investigator	清水慶彦京都大学, 再生医科学研究所, 教授 (00027111)
Co-Investigator(Kenkyū-buntansha)	岩田博夫京都大学, 再生医科学研究所, 教授 (30160120) 中村達雄京都大学, 再生医科学研究所, 助教授 (70227908)
Keywords	組織工学 / 再生医学 / 自己幹細胞 / コラーゲン / 胆管 / 神経再生 / 大腸 / 場の理論
Research Abstract	1)骨髄由来の自己間葉系幹細胞の分離、培養、増殖を続けた。(岩出、中村) 2)末梢血中の幹細胞をPBSCT用いて採取培養するシステムを検討中である。(岩田、中村) 3)立体的マトリックスモデル内での幹細胞との増殖と分化を調べた。(中村、清水) 4)これらの臓器モデルを用いて、人工心臓、人工血管、人工神経、人工食道、人工腸管、人工胆管、人工気管、人工肺、人工膀胱、人工腎臓、人工肝臓、人工内耳細胞、人工膵臓などとして、イヌ、チンチラなどによる動物実験を行い、自己組織の再生状態を観察した。形態的には光顕、特殊染色、電顕などを用いて観察し、機能的には電気生理学的、流体力学的、代謝機能的に観察を続けている。(中村、清水) 5)各種人工組織、人工臓器の再生誘導に役割を果たす幹細胞の分化増殖、因子の解析を行った。(岩田、中村) 6)自己幹細胞による組織再構築のメカニズムを検討している。 7)長期観察例の評価を行った。これらの結果はアメリカ人工臓器学会、国際組織工学会、ヨーロッパ人工臓器学会等で発表した。

Research Products

(30 results)

All Other

All Publications (30 results)

[Publications] 清水慶彦: "再生医学の最先端の課題"月刊新医療. 29. 114-116 (2002)
[Publications] 清水慶彦: "神経再生-現状と展望"外科治療. 86. 38-44 (2002)
[Publications] 清水慶彦: "神経再生"小児内科. 34. 90-93 (2002)
[Publications] 清水慶彦: "再生医療の現状と今後の課題"バイオマテリアル-生体材料-. 20. 175-181 (2002)
[Publications] 清水慶彦: "再生医学とは?"月刊junior. 414. 7-11 (2002)
[Publications] 清水慶彦: "再生医学・医療のフロンティア"週刊医学界新聞. 2468. (2002)
[Publications] 清水慶彦: "気道系の再生"気管支学. 24. 589-594 (2002)
[Publications] 中村達雄: "消化器・呼吸器の再生"週刊医学界新聞. 2505. (2002)
[Publications] 中村達雄: "ここまで進んだ再生医学・医療は補綴治療を根底から変える"補綴臨床. 35. 600-616 (2002)
[Publications] 中村達雄: "どうなる?2013年の再生医療と歯科補綴?"歯科技工. 31. 59-65 (2003)
[Publications] Hagiwara, A.: "Clinical application of PGA-tube for regeneration of intrapelvic nerves during extended surgery for intrapelvic recurrent rectal cancer"Gan To Kagaku Ryoho. 29. 2202-2204 (2002)
[Publications] Ueda, H.: "Use of collagen sponge incorporating transforming growth factor-β1 to promote bone repair in skull defects in rabbits"Biomaterials. 23. 1003-1010 (2002)
[Publications] Ueda, H.: "Repairing of rabbit skull defect by dehydrothermally crosslinked collagen sponges incorporating transforming growth factor β1"J.Control.Release.. (in press).
[Publications] Ueda, H.: "Pepairing of rabbit skull defect by TGF-β1-incorporated collagen sponges of different thickness"J.Biomed.Eng.. (in press).
[Publications] Ueda, H.: "Polyhydroxyalkanonate derivatives in current clinical applications and trials"Adv.Drug.Deliver.Rev. (in press).
[Publications] Komuro, H.: "Application of collagen sponge scaffold to muscular defects of the esophagus : An experimental study in piglets"J.Pediatr.Surg. 37. 1409-1413 (2002)
[Publications] 茂野啓示: "歯科(永久・歯根膜、顎骨)の再生"週刊医学界新聞. 2513. (2002)
[Publications] Shigeno, K.: "Regenerative repair of the mandible using a collagen sponge containing TGF-β1"Int.J.Artif.Organs.. 25. 1095-1102 (2002)
[Publications] Toba, T.: "Influence of dehydrothermal crosslinking on the growth of PC-12 cells cultured on laminin-coated collagen"ASAIO J.. 48. 17-20 (2002)
[Publications] Toba, T.: "Evaluation of peripheral nerve regeneration across an 80-mm gap using a polyglycolic acid (PGA) collagen nerve conduit filled with laminin-soaked collagen sponge in dogs"J.Artif.Organs.. 25. 230-237 (2002)
[Publications] Nakahara, T.: "A novel approach to regeneration of periodontal tissues based on in situ tissue engineering : the effects of bFGF controlled release from a sandwich membrane"Tissue Eng.. (in press).
[Publications] Hayakawa, K.: "Iodinated contrast medium-induced potassium release : The effect of mixing ratios"Radiat.Med. 20. 195-199 (2002)
[Publications] Hori, Y.: "Experimental study on tissue engineering of the small intestine by mesenchymal stem cell seeding"J.Surg.Res.. 102. 156-160 (2002)
[Publications] Hori, Y.: "Functional analysis of the tissue-engineered stomach wall"Artif.Organs.. 26. 868-872 (2002)
[Publications] Liu, Y: "New Type of tracheal bioartificial organ treated with detergent : Maintaining cartilage viability is necessary for successful immunosuppressant-free allotrausplantation"ASAIO J. 48. 21-25 (2002)
[Publications] 中村達雄: "食道"再生医工学の最先端. 170-175 (2002)
[Publications] 鳥羽紀成: "再生組織工学の現状と呼吸器疾患への展望"Annual Review 呼吸器2002. 1-6 (2002)
[Publications] 鳥羽紀成: "末梢神経の再生医療"日本医師会雑誌. (in press).
[Publications] Toba, T.: "Peripheral nerve regeneration using a polyglycolic acid (PGA) collagen nerve conduit filled with collagen sponge : experimental research and human application"Connective Tissue. (in press).
[Publications] 中原貴: "In situ Tissue Engineering による歯周組織の再生"炎症・再生. (印刷中).

2002 Fiscal Year Annual Research Report

自己幹細胞を用いた自己組織再生誘導型バイオ人工臓器

Principal Investigator

清水 慶彦 京都大学, 再生医科学研究所, 教授 (00027111)

Research Products

[Publications] 清水 慶彦: "再生医学の最先端の課題"月刊新医療. 29. 114-116 (2002)

[Publications] 清水 慶彦: "神経再生-現状と展望"外科治療. 86. 38-44 (2002)

[Publications] 清水 慶彦: "神経再生"小児内科. 34. 90-93 (2002)

[Publications] 清水 慶彦: "再生医療の現状と今後の課題"バイオマテリアル-生体材料-. 20. 175-181 (2002)

[Publications] 清水 慶彦: "再生医学とは?"月刊junior. 414. 7-11 (2002)

[Publications] 清水 慶彦: "再生医学・医療のフロンティア"週刊医学界新聞. 2468. (2002)

[Publications] 清水 慶彦: "気道系の再生"気管支学. 24. 589-594 (2002)

[Publications] 中村 達雄: "消化器・呼吸器の再生"週刊医学界新聞. 2505. (2002)

[Publications] 中村 達雄: "ここまで進んだ再生医学・医療は補綴治療を根底から変える"補綴臨床. 35. 600-616 (2002)

[Publications] 中村 達雄: "どうなる?2013年の再生医療と歯科補綴?"歯科技工. 31. 59-65 (2003)

[Publications] Hagiwara, A.: "Clinical application of PGA-tube for regeneration of intrapelvic nerves during extended surgery for intrapelvic recurrent rectal cancer"Gan To Kagaku Ryoho. 29. 2202-2204 (2002)

[Publications] Ueda, H.: "Use of collagen sponge incorporating transforming growth factor-β1 to promote bone repair in skull defects in rabbits"Biomaterials. 23. 1003-1010 (2002)

[Publications] Ueda, H.: "Repairing of rabbit skull defect by dehydrothermally crosslinked collagen sponges incorporating transforming growth factor β1"J.Control.Release.. (in press).

[Publications] Ueda, H.: "Pepairing of rabbit skull defect by TGF-β1-incorporated collagen sponges of different thickness"J.Biomed.Eng.. (in press).

[Publications] Ueda, H.: "Polyhydroxyalkanonate derivatives in current clinical applications and trials"Adv.Drug.Deliver.Rev. (in press).

[Publications] Komuro, H.: "Application of collagen sponge scaffold to muscular defects of the esophagus : An experimental study in piglets"J.Pediatr.Surg. 37. 1409-1413 (2002)

[Publications] 茂野 啓示: "歯科(永久・歯根膜、顎骨)の再生"週刊医学界新聞. 2513. (2002)

[Publications] Shigeno, K.: "Regenerative repair of the mandible using a collagen sponge containing TGF-β1"Int.J.Artif.Organs.. 25. 1095-1102 (2002)

[Publications] Toba, T.: "Influence of dehydrothermal crosslinking on the growth of PC-12 cells cultured on laminin-coated collagen"ASAIO J.. 48. 17-20 (2002)

[Publications] Toba, T.: "Evaluation of peripheral nerve regeneration across an 80-mm gap using a polyglycolic acid (PGA) collagen nerve conduit filled with laminin-soaked collagen sponge in dogs"J.Artif.Organs.. 25. 230-237 (2002)

[Publications] Nakahara, T.: "A novel approach to regeneration of periodontal tissues based on in situ tissue engineering : the effects of bFGF controlled release from a sandwich membrane"Tissue Eng.. (in press).

[Publications] Hayakawa, K.: "Iodinated contrast medium-induced potassium release : The effect of mixing ratios"Radiat.Med. 20. 195-199 (2002)

[Publications] Hori, Y.: "Experimental study on tissue engineering of the small intestine by mesenchymal stem cell seeding"J.Surg.Res.. 102. 156-160 (2002)

[Publications] Hori, Y.: "Functional analysis of the tissue-engineered stomach wall"Artif.Organs.. 26. 868-872 (2002)

[Publications] Liu, Y: "New Type of tracheal bioartificial organ treated with detergent : Maintaining cartilage viability is necessary for successful immunosuppressant-free allotrausplantation"ASAIO J. 48. 21-25 (2002)

[Publications] 中村 達雄: "食道"再生医工学の最先端. 170-175 (2002)

[Publications] 鳥羽 紀成: "再生組織工学の現状と呼吸器疾患への展望"Annual Review 呼吸器2002. 1-6 (2002)

[Publications] 鳥羽 紀成: "末梢神経の再生医療"日本医師会雑誌. (in press).

[Publications] Toba, T.: "Peripheral nerve regeneration using a polyglycolic acid (PGA) collagen nerve conduit filled with collagen sponge : experimental research and human application"Connective Tissue. (in press).

[Publications] 中原 貴: "In situ Tissue Engineering による歯周組織の再生"炎症・再生. (印刷中).

清水慶彦京都大学, 再生医科学研究所, 教授 (00027111)

[Publications] 清水慶彦: "再生医学の最先端の課題"月刊新医療. 29. 114-116 (2002)

[Publications] 清水慶彦: "神経再生-現状と展望"外科治療. 86. 38-44 (2002)

[Publications] 清水慶彦: "神経再生"小児内科. 34. 90-93 (2002)

[Publications] 清水慶彦: "再生医療の現状と今後の課題"バイオマテリアル-生体材料-. 20. 175-181 (2002)

[Publications] 清水慶彦: "再生医学とは?"月刊junior. 414. 7-11 (2002)

[Publications] 清水慶彦: "再生医学・医療のフロンティア"週刊医学界新聞. 2468. (2002)

[Publications] 清水慶彦: "気道系の再生"気管支学. 24. 589-594 (2002)

[Publications] 中村達雄: "消化器・呼吸器の再生"週刊医学界新聞. 2505. (2002)

[Publications] 中村達雄: "ここまで進んだ再生医学・医療は補綴治療を根底から変える"補綴臨床. 35. 600-616 (2002)

[Publications] 中村達雄: "どうなる?2013年の再生医療と歯科補綴?"歯科技工. 31. 59-65 (2003)

[Publications] 茂野啓示: "歯科(永久・歯根膜、顎骨)の再生"週刊医学界新聞. 2513. (2002)

[Publications] 中村達雄: "食道"再生医工学の最先端. 170-175 (2002)

[Publications] 鳥羽紀成: "再生組織工学の現状と呼吸器疾患への展望"Annual Review 呼吸器2002. 1-6 (2002)

[Publications] 鳥羽紀成: "末梢神経の再生医療"日本医師会雑誌. (in press).

[Publications] 中原貴: "In situ Tissue Engineering による歯周組織の再生"炎症・再生. (印刷中).