2003 Fiscal Year Annual Research Report

自己幹細胞を用いた自己組織再生誘導型バイオ人工臓器

Research Project

Project/Area Number	13358016
Research Institution	Kyoto University
Principal Investigator	清水慶彦京都大学, 再生医科学研究所, 教授 (00027111)
Co-Investigator(Kenkyū-buntansha)	岩田博夫京都大学, 再生医科学研究所, 教授 (30160120) 中村達雄京都大学, 再生医科学研究所, 助教授 (70227908)
Keywords	気管 / 組織工学 / 神経 / 再生 / コラーゲン / 足場 / 細胞外マトリックス / マトリックス
Research Abstract	1.臨床応用に用いて、骨髄由来の自己間葉系幹細胞の分化増殖のメカニズムと病変部位における効果が大型動物で解明した。 2.この自己組織再生誘導型バイオ人工臓器は血管、神経、腸管、気管、内耳、喉頭として欠損部位を形態的にも機能的に再生することがビーグル犬やサルなど大型動物で確かめられた。 3.自己幹細胞による組織再構築のメカニズムとしては、組織再生の足場となる細胞外マトリックスの役割が重要であり、細胞の分化誘導をつかさどるsignal moleculeは局所における自己の分泌機能(この多くは誘走してくる白血球系の細胞による)が大きく関与している可能性が強く示唆された。 4.全ての臓器が再生医学の手法で再構築できるわけではないが、なかでも末梢神経や結合織は、我々の開発したin situ Tissue Engineeringの手法で再生させうることが証明された。 5.これらのうち人工気管、人工神経は京都大学医学部附属病院の倫理委員会の審査を経て、2002年より既に患者に使用されている。

Research Products

(41 results)

All Other

All Publications (41 results)

[Publications] 清水慶彦: "組織工学による再生医学"The Lung perspectives. 11. 51-54 (2003)
[Publications] 清水慶彦: "再生医療の成り立ちとそのさまざまな方法"日本医師会雑誌. 129. 299-302 (2003)
[Publications] 清水慶彦: "再生医学の現状と展望-再生医学の歯科への取り組み-"日本歯科医学会誌. 22. 82-85 (2003)
[Publications] 清水慶彦: "再生医学のトピックスとその気管食道領域への応用"日本気管食道科学会会報. 54. 59-63 (2003)
[Publications] 中村達雄: "どうなる?2013年の再生医療と歯科補綴?"歯科技工. 31. 59-65 (2003)
[Publications] 中村達雄: "呼吸器疾患の再生医療"日本医師会雑誌. 129. 369-372 (2003)
[Publications] Nakamura, T.: "Clinical application of nerve conduits consisting of a polyglycolic acid (PGA)-collagen composite tube filled with collagen sponge"Connective Tissue. 35. 53-57 (2003)
[Publications] 中村達雄: "再生医療の技術的側面・Tissue Engineering"CLINICAL NEUROSCIENCE. 21. 1141-1145 (2003)
[Publications] Yamamoto, T.: "Transcatheter arterial embolization using poly-L-lactic acid microspheres"Radiation Medicine. 21. 150-154 (2003)
[Publications] Ito, T.: "Biodegradation of Polyglycolic Acid-Collagen Composite Tubes for nerve Guide in the Peritoneal Cavity"ASAIO Journal. 49. 417-421 (2003)
[Publications] Ito, T.: "Regeneration of hypogastric nerve using a polyglycolic acid (PGA)-collagen nerve conduit filled with collagen sponge proved electrophysiologically in a canine model"Int J Artif Organs. 26. 245-251 (2003)
[Publications] 伊藤忠雄: "人工神経ガイドチューブの腹腔内における分解・吸収性に関する検討"日本炎症・再生医学会雑誌. 23. 275-278 (2003)
[Publications] 稲田有史: "Causalgia(CRPS Type II)の外科的治療-Polyglycolic-acid Collagen(PGA-C) tubeによる生体内再生治療-PAIN RESEARCH"日本疼痛学会誌. 18. 25 (2003)
[Publications] Ueda, H.: "Repairing of rabbit skull defect by dehydrothermally crosslinked collagen sponges incorporating transforming growth factor β1"Journal of Controlled Release. 88. 55-64 (2003)
[Publications] Ueda, H.: "Repairing of rabbit skull defect by TGF-β1-incorporated collagen sponges of different thickness"Journal of Biomedical Engineering. 15. 1-7 (2003)
[Publications] Ueda, H.: "Polyhydroxyalkanonate derivatives in current clinical applications and trials"Advanced Drug Delivery Review. 55. 501-518 (2003)
[Publications] Okamoto, T.: "Cartilage regeneration using slow release of bone morphogenetic protein-2 from a gelatin sponge to treat experimental canine tracheomalacia : A preliminary report"ASAIO Journal. 49. 63-69 (2003)
[Publications] Lee, Y.: "Regeneration of serous membrane on gelatin-processed polyglicolic acid (PGA)-human collagen membrane and its efficacy on the prevention of adhesion"J.Biomed.Mater.Res.. 64. 88-92 (2003)
[Publications] Kanemaru, S.: "Regeneration of the vocal cord using autologous mesenchymal stem cells"Ann Otol Rhinol Laryngol. 112. 915-920 (2003)
[Publications] kanemaru, S.: "Recurrent laryngeal nerve regeneration by tissue engineering"Ann Otol Rhinol Laryngol. 112. 492-498 (2003)
[Publications] Kanemaru, S.: "Regeneration of the mastoid air cells in clinical applications"Acta Otolayngol. (in press).
[Publications] Kuremoto, K.: "Experimental study of regeneration of tooth pulp using in situ Tissue Engineering"Tissue Engineering. 9. 845-846 (2003)
[Publications] 呉本晃一: "in situ Tissue Engineeringによる歯髄再生のための基礎的研究"炎症・再生. 23. 488 (2003)
[Publications] 桜井靖久: "再生医療の現状と将来展望"日本医師会雑誌. 129. 285-298 (2003)
[Publications] Terada, Y.: "Capillary endothelial thrombomodulin expression and fibrin deposition in rats with continuous and bolus lipopolysaccharide administration"Lab Invest. 83. 1165-1173 (2003)
[Publications] 鳥羽紀成: "末梢神経の再生医療"日本医師会雑誌. 129. 323-327 (2003)
[Publications] Toba, T.: "Experimental research on peripheral nerve regeneration using PGA-collagen nerve conduit filled with collagen sponge"Connective Tissue. 35. 45-52 (2003)
[Publications] 鳥羽紀成: "末梢神経.バイオマテリアル-生体材料-"Journal of Japanese Society for Biomaterials. 21. 186-187 (2003)
[Publications] Liu, Y.: "Experimental Study of Blood Typing in Immunosuppressant-free Tracheal Transplantation in Dogs"Thorac Cardiov Surg. 51. 216-220 (2003)
[Publications] 中原貴: "In situ Tissue Engineeringによる歯周組織の再生"Inflammation and Regeneration. 23. 116-121 (2003)
[Publications] Nakahara, T.: "Novel approach to regeneration of periodontal tissues based on in situ tissue engineering : Effects of controlled release of basic fibroblast growth factor from a sandwich membrane"Tissue Engineering. 9. 153-162 (2003)
[Publications] 野村宗吾: "血管再生のための足場材料としての異種血管の力学的性質(Mechanical properties of xenograft used for a scaffold for regeneration of blood vessels)"材料(J.Soc.Mat.Sci., Japan). 9. 309-313 (2003)
[Publications] 萩原明於: "神経再生チューブを用いた神経再建-直腸がん手術における神経再生チューブの臨床応用-"最新医学. 58. 108-113 (2003)
[Publications] Hori, Y.: "Effect of basic fibroblast growth factor on vascularization in esophagus tissue engineering"Int J Artif Organs. 26. 241-244 (2003)
[Publications] Matsuno, T.: "In vivo osteogenetic potential bone marrow mesenchymal stem cell on β-tricalcium phosphate (β-TCP)/collagen sponge"ASAIO J. 49. 209 (2003)
[Publications] Matsuno, T.: "Fabrication of β-TCP/collagen sponge for bone regeneration"Int J Artif Organs. 26. 638 (2003)
[Publications] 松野智宣: "自家成長因子を用いたβ-TCP/コラーゲンスポンジによる骨再生"炎症・再生. 23. 502 (2003)
[Publications] Naito, Y.: "Transplantation of bone marrow stromal cells into the cochlea of chinchillas"Neuro Report. 15. 1-4 (2004)
[Publications] Nakamura, T.: "Sterilized, freeze-dried amniotic membrane ; A useful substrate for ocular surface reconstruction"Invest Ophthalmol Vis Sci. 45. 93-99 (2004)
[Publications] Okamoto, T.: "Slow release of bone morphogenetic protein (BMP)-2 from a gelatin sponge promotes regeneration of tracheal cartilage"J Thorac Cardiovasc Surg.. 127(2). 329-334 (2004)
[Publications] 中村達雄: "「ここまで進んだ再生医療の実際」場の理論を用いた食道の再生"田畑泰彦/編、羊土社. 129-134 (2003)

2003 Fiscal Year Annual Research Report

自己幹細胞を用いた自己組織再生誘導型バイオ人工臓器

Principal Investigator

清水 慶彦 京都大学, 再生医科学研究所, 教授 (00027111)

Research Products

[Publications] 清水慶彦: "組織工学による再生医学"The Lung perspectives. 11. 51-54 (2003)

[Publications] 清水慶彦: "再生医療の成り立ちとそのさまざまな方法"日本医師会雑誌. 129. 299-302 (2003)

[Publications] 清水慶彦: "再生医学の現状と展望-再生医学の歯科への取り組み-"日本歯科医学会誌. 22. 82-85 (2003)

[Publications] 清水慶彦: "再生医学のトピックスとその気管食道領域への応用"日本気管食道科学会会報. 54. 59-63 (2003)

[Publications] 中村達雄: "どうなる?2013年の再生医療と歯科補綴?"歯科技工. 31. 59-65 (2003)

[Publications] 中村達雄: "呼吸器疾患の再生医療"日本医師会雑誌. 129. 369-372 (2003)

[Publications] Nakamura, T.: "Clinical application of nerve conduits consisting of a polyglycolic acid (PGA)-collagen composite tube filled with collagen sponge"Connective Tissue. 35. 53-57 (2003)

[Publications] 中村達雄: "再生医療の技術的側面・Tissue Engineering"CLINICAL NEUROSCIENCE. 21. 1141-1145 (2003)

[Publications] Yamamoto, T.: "Transcatheter arterial embolization using poly-L-lactic acid microspheres"Radiation Medicine. 21. 150-154 (2003)

[Publications] Ito, T.: "Biodegradation of Polyglycolic Acid-Collagen Composite Tubes for nerve Guide in the Peritoneal Cavity"ASAIO Journal. 49. 417-421 (2003)

[Publications] Ito, T.: "Regeneration of hypogastric nerve using a polyglycolic acid (PGA)-collagen nerve conduit filled with collagen sponge proved electrophysiologically in a canine model"Int J Artif Organs. 26. 245-251 (2003)

[Publications] 伊藤忠雄: "人工神経ガイドチューブの腹腔内における分解・吸収性に関する検討"日本炎症・再生医学会雑誌. 23. 275-278 (2003)

[Publications] 稲田有史: "Causalgia(CRPS Type II)の外科的治療-Polyglycolic-acid Collagen(PGA-C) tubeによる生体内再生治療-PAIN RESEARCH"日本疼痛学会誌. 18. 25 (2003)

[Publications] Ueda, H.: "Repairing of rabbit skull defect by dehydrothermally crosslinked collagen sponges incorporating transforming growth factor β1"Journal of Controlled Release. 88. 55-64 (2003)

[Publications] Ueda, H.: "Repairing of rabbit skull defect by TGF-β1-incorporated collagen sponges of different thickness"Journal of Biomedical Engineering. 15. 1-7 (2003)

[Publications] Ueda, H.: "Polyhydroxyalkanonate derivatives in current clinical applications and trials"Advanced Drug Delivery Review. 55. 501-518 (2003)

[Publications] Okamoto, T.: "Cartilage regeneration using slow release of bone morphogenetic protein-2 from a gelatin sponge to treat experimental canine tracheomalacia : A preliminary report"ASAIO Journal. 49. 63-69 (2003)

[Publications] Lee, Y.: "Regeneration of serous membrane on gelatin-processed polyglicolic acid (PGA)-human collagen membrane and its efficacy on the prevention of adhesion"J.Biomed.Mater.Res.. 64. 88-92 (2003)

[Publications] Kanemaru, S.: "Regeneration of the vocal cord using autologous mesenchymal stem cells"Ann Otol Rhinol Laryngol. 112. 915-920 (2003)

[Publications] kanemaru, S.: "Recurrent laryngeal nerve regeneration by tissue engineering"Ann Otol Rhinol Laryngol. 112. 492-498 (2003)

[Publications] Kanemaru, S.: "Regeneration of the mastoid air cells in clinical applications"Acta Otolayngol. (in press).

[Publications] Kuremoto, K.: "Experimental study of regeneration of tooth pulp using in situ Tissue Engineering"Tissue Engineering. 9. 845-846 (2003)

[Publications] 呉本晃一: "in situ Tissue Engineeringによる歯髄再生のための基礎的研究"炎症・再生. 23. 488 (2003)

[Publications] 桜井靖久: "再生医療の現状と将来展望"日本医師会雑誌. 129. 285-298 (2003)

[Publications] Terada, Y.: "Capillary endothelial thrombomodulin expression and fibrin deposition in rats with continuous and bolus lipopolysaccharide administration"Lab Invest. 83. 1165-1173 (2003)

[Publications] 鳥羽紀成: "末梢神経の再生医療"日本医師会雑誌. 129. 323-327 (2003)

[Publications] Toba, T.: "Experimental research on peripheral nerve regeneration using PGA-collagen nerve conduit filled with collagen sponge"Connective Tissue. 35. 45-52 (2003)

[Publications] 鳥羽紀成: "末梢神経.バイオマテリアル-生体材料-"Journal of Japanese Society for Biomaterials. 21. 186-187 (2003)

[Publications] Liu, Y.: "Experimental Study of Blood Typing in Immunosuppressant-free Tracheal Transplantation in Dogs"Thorac Cardiov Surg. 51. 216-220 (2003)

[Publications] 中原 貴: "In situ Tissue Engineeringによる歯周組織の再生"Inflammation and Regeneration. 23. 116-121 (2003)

[Publications] Nakahara, T.: "Novel approach to regeneration of periodontal tissues based on in situ tissue engineering : Effects of controlled release of basic fibroblast growth factor from a sandwich membrane"Tissue Engineering. 9. 153-162 (2003)

[Publications] 野村宗吾: "血管再生のための足場材料としての異種血管の力学的性質(Mechanical properties of xenograft used for a scaffold for regeneration of blood vessels)"材料(J.Soc.Mat.Sci., Japan). 9. 309-313 (2003)

[Publications] 萩原明於: "神経再生チューブを用いた神経再建-直腸がん手術における神経再生チューブの臨床応用-"最新医学. 58. 108-113 (2003)

[Publications] Hori, Y.: "Effect of basic fibroblast growth factor on vascularization in esophagus tissue engineering"Int J Artif Organs. 26. 241-244 (2003)

[Publications] Matsuno, T.: "In vivo osteogenetic potential bone marrow mesenchymal stem cell on β-tricalcium phosphate (β-TCP)/collagen sponge"ASAIO J. 49. 209 (2003)

[Publications] Matsuno, T.: "Fabrication of β-TCP/collagen sponge for bone regeneration"Int J Artif Organs. 26. 638 (2003)

[Publications] 松野智宣: "自家成長因子を用いたβ-TCP/コラーゲンスポンジによる骨再生"炎症・再生. 23. 502 (2003)

[Publications] Naito, Y.: "Transplantation of bone marrow stromal cells into the cochlea of chinchillas"Neuro Report. 15. 1-4 (2004)

[Publications] Nakamura, T.: "Sterilized, freeze-dried amniotic membrane ; A useful substrate for ocular surface reconstruction"Invest Ophthalmol Vis Sci. 45. 93-99 (2004)

[Publications] Okamoto, T.: "Slow release of bone morphogenetic protein (BMP)-2 from a gelatin sponge promotes regeneration of tracheal cartilage"J Thorac Cardiovasc Surg.. 127(2). 329-334 (2004)

[Publications] 中村達雄: "「ここまで進んだ再生医療の実際」場の理論を用いた食道の再生"田畑泰彦/編、羊土社. 129-134 (2003)

清水慶彦京都大学, 再生医科学研究所, 教授 (00027111)

[Publications] 中原貴: "In situ Tissue Engineeringによる歯周組織の再生"Inflammation and Regeneration. 23. 116-121 (2003)