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2003 Fiscal Year Final Research Report Summary

Investigation on the mechanisms of wilting and host defenses in mass mortality of oaks

Research Project

Project/Area Number 13460064
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field 林学
Research InstitutionThe University of Tokyo

Principal Investigator

YAMADA Toshihiro  The University of Tokyo, Graduate School of Agricultural and Life Sciences, Assistant Professor, 大学院・農学生命科学研究科, 助教授 (30332571)

Co-Investigator(Kenkyū-buntansha) ITO Shin-ichiro  Mie University, Faculty of Bioresources, Assistant Professor, 生物資源学部, 助教授 (90092139)
SUZUKI Kazuo  The University of Tokyo, Graduate School of Agricultural and Life Sciences, Professor, 大学院・農学生命科学研究科, 教授 (80162931)
SAKAUE Daisuke  The University of Tokyo, Graduate School of Agricultural and Life Sciences, Assistant, 大学院・農学生命科学研究科, 助手 (90313080)
Project Period (FY) 2001 – 2003
Keywordsoak trees / mass mortality / Platypus quercivorus / Raffaelea quercivora / transpiration / blockage of water conduction / defense responses / susceptibility
Research Abstract

Mechanisms of wilting
Water conductivity was conspicuously reduced near the inoculation site of oak seedlings inoculated with the pathogenic fungus Raffaelea quercivora, while the conductivity did not change in the distal part. Wilt development was delayed in both inoculated seedlings and excised seedlings when water was supplied to the distal part, suggesting local water blockage as the wilt mechanism. Transpiration decreased in the early stage of wilting after the fungal inoculation. No relationship was observed between axial spread of wood discoloration / fungi and wilt development. Although water content decreased in very adjacent area to the inoculation site, the area without water conduction extended further from dry zone, suggesting that no marked cavitation occur in this disease. Tyloses were observed in vessels which passed through the area without water conduction as the factor of sap ascent blockage.
Defense responses
Although antifungal activity of extracts did not increase in … More reaction zone barrier, deposits lining or occluding xylem elements were remarkable. Lignin-like compound and pectin were major constituents in fiber region and in small vessel and tracheid region, respectively. Phenols, quinones and lipids were also observed. Fiber occlusion in the xylem was observed 3 days after the inoculation, and spatially continuous barrier was formed after one week. Tyloses, suberization and phenols accumulation became remarkable after 2 weeks. Lignin content increased at the reaction zone barrier. Most of increased lignin was attributed to condensed lignin, probably originated from guaiacyl-rich lignin.
Characteristics in the responses of susceptible and resistant trees
To investigate the differences in defense responses among Fagaceae species, seedlings of several species were inoculated with R.quercivora. Only Quercus serrata and Q.crispula seedlings wilted. Differences in susceptibility coincided with tangential spread of wood discoloration, but not with the axial spread. Less

  • Research Products

    (59 results)

All Other

All Publications (59 results)

  • [Publications] Ito, S: "Japanese oak wilt caused by Raffaelea quercivora carried by an ambrosia beetle"Forest Insect Population Dynamics and Host Influences", Proceeding of the IUFRO joint meeting in Kanazawa 2003. (印刷中). (2004)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kasai, M: "Study on extractives of Quercus crispula wood damaged by Platypus quercivorus attack"Forest Insect Population Dynamics and Host Influences", Proceeding of the IUFRO joint meeting in Kanazawa 2003. (印刷中). (2004)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kato, K.: "Induced response of oak trees to Raffaelea quercivora as a possible control tool against Japanese oak wilt caused by the ambrosia fungus carried by an ambrosia beetle"Forest Insect Population Dynamics and Host Influences", Proceeding of the IUFRO joint meeting in Kanazawa 2003. (印刷中). (2004)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 木村匡子: "核rDNAのITS領域を用いたRaffaelea quercivoraのPCR-RFLP解析"中部森林研究. 52(印刷中). (2004)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 小穴久仁: "ミズナラ樹皮に塗布したガロ酸・エラグ酸に対するカシノナガキクイムシの応答"中部森林研究. 52(印刷中). (2004)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 村田政穂: "Raffaelea quercivoraに対するブナ科3樹種の感受性の差異"中部森林研究. 52(印刷中). (2004)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Oana, H: "Reaction of ambrosia beetle Platypus quercivorus to gallic acid and ellagic acid in oak sapwood"Forest Insect Population Dynamics and Host Influences", Proceeding of the IUFRO joint meeting in Kanazawa 2003. (印刷中). (2004)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Takemoto, H.: "Volatile compounds related to attractant of Platypus quercivorus from Quercus mongolica"Forest Insect Population Dynamics and Host Influences", Proceeding of the IUFRO joint meeting in Kanazawa 2003. (印刷中). (2004)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yamada, T.: "Detection of wood discoloration in a canker fungus-inoculated Japanese cedar by neutron radiography"Journal of Radioanalytical and Nuclear Chemistry. (印刷中). (2004)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yamada, T.: "Defense responses of oak trees against the fungus Raffaelea quercivora vectored by the ambrosia beetle Platypus quercivorus"Forest Insect Population Dynamics and Host Influences", Proceeding of the IUFRO joint meeting in Kanazawa 2003. (印刷中). (2004)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yamato, M.: "Water relations of Quercus mongolica seedlings inoculated with Raffaelea quercivora : ambrosia fungi related with mass mortality of oaks in Japan"Forest Insect Population Dynamics and Host Influences", Proceeding of the IUFRO joint meeting in Kanazawa 2003. (印刷中). (2004)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 伊藤進一郎: "アメリカ植物病理学会2003年年次大会"ナラ・カシ類衰退・枯死に関するシンポジウム"に参加して"樹木医学研究. 7. 99-103 (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 木村匡子: "Raffaelea quercivora菌株間の形態学的特徴"中部森林研究. 51. 199-200 (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 小穴久仁: "カシノナガキクイムシの穿孔によるミズナラの壊死変色部と健全材との成分の比較"中部森林研究. 51. 189-190 (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 伊藤進一郎: "現在問題となっているブナ科樹木の衰退、枯死"森林科学. 35. 4-9 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 伊藤進一郎: "ナラ枯れ被害に関連する菌類と枯死機構"森林科学. 35. 35-40 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 伊藤進一郎: "ナラ類、シイ・カシ類の枯死被害に関連する菌類と枯死機構"山林. 1422. 54-60 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 伊藤進一郎: "カシノナガキクイムシの形態の地域間比較"中部森林研究. 50. 87-88 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 梶村 恒: "人工飼料を利用したカシノナガキクイムシの飼育の試み"中部森林研究. 50. 89-92 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kubono, T.: "Raffaelea quercivora sp.nov. associated with mass mortality of Japanese oak, and the ambrosia beetle (Platypus quercivorus)"Mycoscience. 43. 255-260 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 村田政穂: "Raffaelea属菌に対するブナ科6樹種の感受性の差異"中部森林研究. 50. 107-108 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 中根阿沙子: "コナラに対するRaffaelea属菌接種法の検討"中部森林研究. 50. 109-112 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 奥田章子: "ナラ類集団枯損被害発生林分におけるコナラ・ミズナラ根系について-カシノナガキクイムシの加害程度と外生菌根の形成状況との関係-"中部森林研究. 50. 83-86 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 大和万里子: "ナラ類の萎凋枯死と通水阻害"東京大学演習林報告. 106. 69-76 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ito, S.: "Pathogenicity of Raffaelea quercivora associated with mass mortality of fagaceous trees in Japan"8th ICPP Vol.2 Offered papers. 155 (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ito, S.: "Massive mortality of Fagaceous trees in Japan"Phytopathology. 93. S102 (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yamada, T.: "Defense responses of oak sapwood in relation to wilt of oak trees in Japan"8th ICPP Vol.2 Offered papers. 155 (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yamada, T.: "Mechanism of wilting in oak mortality in Japan caused by Raffaelea quercivora"IMC7 Book of Abstracts. 276 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yamato, M.: "Distribution of Ceratocystis fagacearum in actively wilting red oaks"Phytopathology. 92. S88 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 伊藤進一郎: "ナラ類の萎凋病.森林保護学(鈴木和夫編著)"朝倉書店(印刷中). (2004)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 伊藤進一郎: "ナラ・カシ類の枯死被害に関連する菌類とその病原性.森林をまもる-森林防疫50年の成果と今後の展望"全国森林病虫獣害防除協会. 493 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ito, S.: "Japanese oak wilt caused by Raffaelea quercivora carried by an ambrosia beetle""Forest Insect Population Dynamics and Host Influences", Proceeding of the IUFRO joint meeting in Kanazawa 2003. (in press). (2004)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kasai, M.: "Study on extractives of Quercus crispula wood damaged by Platypus quercivorus attack""Forest Insect Population Dynamics and Host Influences", Proceeding of the IUFRO joint meeting in Kanazawa 2003. (in press). (2004)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kato, K.: "Induced response of oak trees to Raffaelea quercivora as a possible control tool against Japanese oak wilt caused by the ambrosia fungus carried by an ambrosia beetle""Forest Insect Population Dynamics and Host Influences", Proceeding of the IUFRO joint meeting in Kanazawa 2003. (in press). (2004)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kimura, K.: "Phylogenetic analysis of Raffaelea quercivora using PCR-RFLP of rDNA ITS region"Chubu Forestry Research. 52(in press). (2004)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Oana, H.: "Reaction of ambrosia beetle, Platypus quercivorus, to gallic acid and ellagic acid applied on oak bark"Chubu Forestry Research. 52(in press). (2004)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Murata, M.: "Differences in the sensitivity among three species of the Fagaceae to Raffaelea sp."Chubu Forestry Research. 52(in press). (2004)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Oana, H.: "Reaction of ambrosia beetle Platypus quercivorus to gallic acid and ellagic acid in oak sapwood""Forest Insect Population Dynamics and Host Influences", Proceeding of the IUFRO joint meeting in Kanazawa 2003. (in press). (2004)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Takemoto, H.: "Volatile compounds related to attractant of Platypus quercivorus from Quercus inongolica""Forest Insect Population Dynamics and Host Influences", Proceeding of the IUFRO joint meeting in Kanazawa 2003. (in press). (2004)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yamada, T.: "Detection of wood discoloration in a canker fungus-inoculated Japanese cedar by neutron radiography"Journal of Radioanalytical and Nuclear Chemistry. (in press). (2004)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yamada, T.: "Defense responses of oak trees against the fungus Raffaelea quercivora vectored by the ambrosia beetle Platypus quercivorus""Forest Insect Population Dynamics and Host Influences", Proceeding of the IUFRO joint meeting in Kanazawa 2003. (in press). (2004)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yamato, M.: "Water relations of Quercus mongolica seedlings inoculated with Raffaelea quercivora; ambrosia fungi related with mass mortality of oaks in Japan.""Forest Insect Population Dynamics and Host Influences", Proceeding of the IUFRO joint meeting in Kanazawa 2003,. (in press). (2004)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ito, S.: "A report from the symposium 'On the wilt and death of oak trees" held at the 2003 Annual Meeting of the American Phytopathological Society"Journal of Tree Health. 7(2). 99-103 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kimura, K.: "Comparison of morphological characteristics on cultural media in Raffaelea quercivora isolates"Chubu Forestry Research. 51. 199-200 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Oana, H.: "Comparison of chemical composition of xylem extract between healthy sapwood and necrosis caused by an ambrosia beetle Platypus quercivorus (Murayama) (Coleoptera : Platypodidae) the previous year"Chubu Forestry Research. 51. 189-190 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ito, S.: "Decline and mortality of Fagaceae species becoming a serious problem"Forest Science. 35. 4-9 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ito, S.: "Fungi associated with mass mortality of oak trees in Japan and wilting mechanisms"Forest Science. 35. 35-40 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ito, S.: "Fungi associated with mass mortality of Fagaceous trees in Japan and wilting mechanisms"Sanrin. 1422. 54-60 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ito, S.: "Comparison of emergence periods and morphology of Platypus quercivorus among three different sites"Chubu Forestry Research. 50. 87-88 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kajimura, H.: "Rearing of Platypus quercivorus in a semi-artificial diet"Chubu Forestry Research. 50. 89-92 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kubono, T.: "Raffaelea quercivora sp. nov, associated with mass mortality of Japanese oak, and the ambrosia beetle (Platypus quercivorus)"Mycoscience. 43(3). 255-260 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Murata, M.: "Differences in the sensitivity among six species of the Fagaceae to Raffaelea sp."Chubu Forestry Research. 50. 107-108 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nakane, A.: "The examination of inoculation methods by Raffaelea sp. to Querus serrata"Chubu Forestry Research. 50. 109-112 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Okuda, A.: "Root systems of Quercus serrata and Q. mongolica var. grosseserrata in the mass mortality of an oak forest -The relationship between damage by Platypus quercivorus and formation of ectomycorrhizal roots -"Chubu Forestry Research. 50. 83-86 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yamato, M.: "Wilt of oaks -Blockage of xylem sap ascent"Bullettin of Tokyo University Forests. 106. 69-76 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ito, S.: "Pathogenicity of Raffaelea quercivora associated with mass mortality of fagaceous trees in Japan"8th ICPP Vol.2 Offered papers;. 155. (2003)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ito, S.: "Massive mortality of Fagaceous trees in Japan"Phytopathology. 92(6). S88 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ito, S.: "Wilt disease of oaks"Forest Protection (Suzuki, K. ed.) (Asakura Shoten). (in press). (2004)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ito, S.: "Fungi associated with mass mortality of oak trees and their pathogenicity"Protect the Forest -Resuts of 50 years of forest protection and future prospect, 493p. (Association for Forest Protection). 87-95 (2002)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2005-04-19  

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