Co-Investigator(Kenkyū-buntansha) |
ITABASHI Yuji Keio University, School of Medicine, Assistant, 医学部, 助手 (00317108)
MIYOSHI Shunichiro Keio University, School of Medicine, Assistant, 医学部, 助手 (10296577)
FUJITA Jun Keio University, School of Medicine, Assistant, 医学部, 助手 (10306706)
YAGI Takashi Keio University, School of Medicine, Assistant, 医学部, 助手 (40317136)
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Research Abstract |
In order to obtain regenerative cardiomyocyte from bone marrow, we had developed a method to isolate mesenchymal stem cell from bone marrow. Using autoMACS, CD45-, CD29+, CD34-, CD44+ cells were isolated from fresh whole bone marrow cells, and were seeded on the fibronectin-coated dishes. FACS analysis revealed that these cells also showed CD 13-, and CD49e+. These cells could proliferate in culture, and differentiated into the adipocytes under the medium containing insulin. Moreover, these cells also differentiated into osteoblast under the condition of dexamethazone, ascorbic acid, and β-glyceroposphates. IGF-1, FGF, TGF-b, LIF, EGF, nor endotelin-1 did not augment cardimoyocyte differentiation in these cells. However, Wnt11 augmented cardiomyocyte differentiation in these cells.
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