2003 Fiscal Year Final Research Report Summary
Transplantation of hepatocyte-like cells differentiated from ES cells
| Project/Area Number |
13470232
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General surgery
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| Research Institution | Tokyo Medical and Dental University |
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Principal Investigator |
TERAMOTO Kenichi Tokyo Medical and Dental Uni., surgery, Associate Professor, 大学院・医歯学総合研究科, 助教授 (80197813)
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| Co-Investigator(Kenkyū-buntansha) |
TAKASE Kzo Tokyo Medical and Dental Uni., Professor, 大学院・医歯学総合研究科, 教授 (90211333)
TERAOKA Hirofumi Tokyo Medical and Dental Uni., M.R.I., Professor, 難治疾患研究所, 教授 (30019137)
ARII Shigeki Tokyo Medical and Dental Uni., surgery, Professor, 大学院・医歯学総合研究科, 教授 (50151171)
SAITO Keiko Tokyo Medical and Dental Uni., Lecturer, 難治疾患研究所, 教務職員 (50178969)
TANAK Yujiro Tokyo Medical and Dental Uni., Professor, 医学部附属病院, 教授 (70236644)
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| Project Period (FY) |
2001 – 2003
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| Keywords | mouse embryonic stern cell / hepatocyte differentiation / umbilical cord blood / cell transplantation / liver failure / cell differentiation |
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| Research Abstract |
1)A study of induction of hepatocytefrom ES cell
We have succeeded in inducing albumin-producing cell through EB from ES cell. These cell had a ability of producing urea. Taken these together, these cells are considered to be hepatocyte. This hepatocyte-like cell was transplanted into C57BL/6 mouse which had liver injury in advance. The transplanted cell grew up in the recipient liver and produced albumin. But, teratoma formation was seen in 20-30% mouse. The cell was selected in order to suppress teratoma. First, the single layer cell culture from EB was performed, and isolated. The cell was selected by specific gravity by the ercoll method. This cell group was negative in oct 3/4. When this cell was transplanted to an above-mentioned liver injury model mouse, teratoma formation was not recognized. As mentioned above, recovery of the cell group which does not generate teratoma from an embryonic stem cell was attained. Furthermore, it became possible by removing other blood cell ingredients for this cell group using FAGS and MACS to obtain many albumin positive cells.
2) ES cell of cynomolgus monkey
Aiming at clinical application of embryonic stem cell, induction of a liver cell was tried from cynomolgus monkey embryonic stem cell. Although a different method of induction from a mouse embryonic stem cell was required for induction of cynomolgus monkey stem cell, albumin-producing cell was able to be induced by our method.
3) Induction of hepatocyte from umbilical cord blood
We succeeded in liver cell induction from umbilical cord blood. Two cell types were recognized, one is expressing albumin and CK-18 and the other is expressing albumin and CK-19. Now, we are studying bile duct epithelium cell induction from umbilical cord blood.
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Research Products
(11 results)
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[Publications] Chinzei R, Tanaka Y, Shimizu-Saito K, Hara Y, Kakinuma S, Watanabe M, Teramoto K, Arii S, Takase IK, Sato C, Terada N, Teraoka H: "Embryoid-body cells derived from a mouse embryonic stem cell line show differentiation into functional hepatocytes."Hepatology. 36. 22-29 (2002)
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「研究成果報告書概要(和文)」より
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[Publications] Kakinuma S, Tanaka Y, Chinzei R, Watanabe M, Shimizu-Saito K, Hara Y, Teramoto K, Arii S, Sato C, Takase K, Yasumizu T, Teraoka H: "Human umbilical blood as a source of transplantable hepatic progenitor cells."Stem Cells. 21. 217-227 (2003)
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[Publications] Kakinuma S, Tanaka Y, Chinzei R, Watanabe M, Shimizu-Saito K, Hara Y, Teramoto K, Arii S, Sato C, Takase K, Yasumizu T, Teraoka H: "Human umbilical cord blood as a source of transplantable hepatic progenitor cells."Stem Cells 21. 21. 217-227 (2003)
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「研究成果報告書概要(欧文)」より
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[Publications] Ozeki R, Kakinuma S, Shimizu-Saito K, Tanaka, Y, Teramoto.K, Arii S, Takase K, Yasumizu T, Asahina K, Teraoka H: "Functional dendritic cells derived from human umbilical cord blood in co-culture with a hepatic stellate cell line L190."(in preparation).
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「研究成果報告書概要(欧文)」より
