2003 Fiscal Year Final Research Report Summary
Development for DNA vaccination against viral hepatitis recurrence after the liver transplantation using gene gun
| Project/Area Number |
13470261
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Digestive surgery
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| Research Institution | Jichi Medical University |
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Principal Investigator |
KOBAYASHI Eiji Jichi Medical School, School of Medicine, Professor, 医学部, 教授 (00245044)
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| Co-Investigator(Kenkyū-buntansha) |
MURAKAMI Takashi Jichi Medical School, School of Medicine, Assistant Professor, 医学部, 講師 (00326852)
KAWAMATA Yoji Jichi Medical School, School of Medicine, Assistant Professor, 医学部, 講師 (00218380)
OKAMOTO Hiroaki Jichi Medical School, School of Medicine, Professor, 医学部, 教授 (30177092)
TANAKA Koichi Kyoto University, Graduate School of Medicine, Professor, 大学院・医学研究科, 教授 (20115877)
KANEKO Takashi Jichi Medical School, School of Medicine, Assistant Professor, 医学部, 助手 (10254913)
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| Project Period (FY) |
2001 – 2003
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| Keywords | Viral hepatitis / Liver transplantation / DNA vaccine / Non-specific immunosuppressant / KF506 / Hydrodynamic DNA injection / Gene gun |
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| Research Abstract |
The DNA vaccine represents a novel means of expressing antigens in vivo for the generation of both humoral and cellular immune responses. It has elicited protective immunity in a number of preclinical models of diseases. In the light of efficient DNA vaccination, particle-mediated gene transfer (gene gun) was one of the most immunization methods. Although recent advances in gene therapy have improved a number of recombinant viral vectors, their recombinant viruses may remain restricted from the standpoint of clinical application because of the risk of systemic complications in the transplant recipient. In particular, we demonstrated a preventive potential of DNA vaccine against hepatitis B virus in a rat model of the liver transplantation. Preliminary evidence in this preclinical model suggests that the combination of an immunized partial liver graft with FK506 strongly enhance antibody production against hepatitis B virus surface antigen. Therefore, DNA immunization could provide an effective strategy for introducing protective immune response against infectious diseases in the liver transplantation.
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