MIYACHI Shigeru Nagoya University, Graduate School of Medicine, Associate Professor, 大学院・医学系研究科, 助教授 (00293697)
WAKABAYASHI Toshihiko Nagoya University Hospital, Associate Professor, 医学部附属病院, 助教授 (50220835)
YOSHIDA Jun Nagoya University, Graduate School of Medicine, Professor, 大学院・医学系研究科, 教授 (40158449)
"Lipo-signal" is a way to increase and transduce some kinds of signals by combining a lot of biological materials with lipsomes. In this study, we basically studied a development of therapy on central nervous system (CNS) diseases using "Lipo-signal"
Subject 1 : Development of a new immunotherapy for brain tumors using "Lipo-signal"
(1) Lipo-signal, which consisted of small unilamellar vesicle-type liposomes and cell-extracts from cultured glioma cells, induced a specific immune response for the tumor cells in vitro and in vivo. In an experimental mouse melanoma, it induced a strong immune response, even in a lot of metastatic lesions.
(2) Expressions of CD80 and CD86 on dendritic cells (DCs) significantly increased by co-culture DCs with Lipo-signal mentioned above.
Subject 2 : Development of a new regenerative medicine using "Lipo-signal"
Lipo-signal, which consisted of small unilamellar vesicle-type liposomes and biological materials such as VEGF, FGF, NGF, GDNF, and bcl-2, promoted neuronal development of neural stem cells.
Subject 3. Administration of "Lipo-signal"
(1) Intravascualr approach : We injected Lipo-signal into CNS vessels through intravascular catheter and investigated the transfer to brain. Unfortunately, little lipo-signal transferred into brain, but the transfer significantly increased by lactocerebroside-conjugated lipo-signal. Lactocerebroside-conjugated lipo-signal may play a role as a carrier to CNS.
(2) Intracellular dynamics : Lipid entered into the cells through endocytsis and reached to the perinuclear region within 1 h.Thereafter, some were decomposed and others bound to host chromosome during cell division.
From these findings, it is suggested that "Lipo-signal" may play a role on development of new therapy for CNS diseases.