2002 Fiscal Year Final Research Report Summary
Transplantation of stem cells from sympathetic ganglion into the brain Parkinson disease
| Project/Area Number |
13470298
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Cerebral neurosurgery
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| Research Institution | Wakayama Medical University |
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Principal Investigator |
ITAKURA Toru Wakayama Medical University, Medicine Professor and Chairman, 医学部, 教授 (40100995)
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| Co-Investigator(Kenkyū-buntansha) |
NAKAO Naoyuki Wakayama Medical University, Medicine Assistant Professor, 医学部, 講師 (30301435)
OOIWA Yoshitsugu Wakayama Medical University, Medicine Assistant Professor, 医学部, 講師 (30322374)
UEMATSU Yuji Wakayama Medical University, Medicine Assistant Professor, 医学部, 講師 (90223502)
OGURA Mitsuhiro Wakayama Medical University, Medicine Assistant Professor, 医学部, 助手 (90326364)
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| Project Period (FY) |
2001 – 2002
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| Keywords | Parkinson disease / Stem cell / Sympathetic ganglion / Transplantation |
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| Research Abstract |
Stem cell has multipotent and self renewal ability, which is ideal source of donor for transplantation. We have studied transplantation of sympathetic ganglion into the brain with Parkinson disease, showing effect on parkinsonian symptoms such as bradykinesia and muscle rigidity. In the present research project we have applied transplantation of stem cells for Parkinson disease and examined effect of transplantation on parkinsonian symptoms. We also studied clinical research of sympathetic ganglion transplantation in the brain with Parkinson patients. Neural stem cells from nestin-GFP(green fluorescence protein) mouse were transplanted into the brain of 6-OHDA injected rat(an animal model of Parkinson disease). Transplanted stem cells well survived in the host brain. They increased cell numbers and extended their axons in the host brain. Surviving cells expressed TH imnmnoreactivity in the host brain. Survival rate in this experiment were 6-10%. Rotational scores after amphetamine administration were significantly reduced (77.3% on average) in the transplanted rats. Four patients undergoing thoracic sympathetic ganglia were carefully observed for long period, showing significant reduction in the percentage of time spent in the off phase. Piece of ganglion cultured in vitro revealed TH-, VMAT- and AADC- immunoreactivity.
This research project clearly demonstrated the effects of stem cell transplantation on symptoms in animal model of Parkinson disease and parkinsonian patients.
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Research Products
(16 results)
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[Publications] K Sawamoto, N Nakao, K Kobayashi, N Matsushita, H Takahashi, K Kakishita, A Yamamoto, J Yoshizaki, J Terashima, F Murakami, J Itakura, H Okano: "Usualization, direct isolation, and transplantation of midbrain dopaminergic neurons labeled with a TH-GFP transgene"Proc. Natl acad sci USA. 98. 6423-6428 (2001)
Description
「研究成果報告書概要(欧文)」より
