2002 Fiscal Year Final Research Report Summary
Molecular biological and molecular genetic studies in apoptosis of retinal ganglion cells and retinal differentiation.
| Project/Area Number |
13470368
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Ophthalmology
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| Research Institution | HIROSHIMA UNIVERSITY |
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Principal Investigator |
MISHIMA Hiromu Hiroshima University, Graduate School of Biomedical Sciences, Professor, 大学院・医歯薬学総合研究科, 教授 (20034100)
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| Co-Investigator(Kenkyū-buntansha) |
OKADA Koji Hiroshima University, Medical Hospital, Assistant Professor, 医学部附属病院, 講師 (80294578)
MINAMOTO Atsushi Hiroshima University, Graduate School of Biomedical Sciences , Associate Professor, 大学院・医歯薬学総合研究科, 助教授 (10253072)
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| Project Period (FY) |
2001 – 2002
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| Keywords | glaucoma / retinal ganglion cell death / nitric oxide / nitric oxide synthase / BDNF / SAM mouse / NMDA receptor / antibody |
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| Research Abstract |
The expression of isoforms of nitric oxide sythase (NOS), enzymes responsible for NO production, and the synthesis of nitric oxide (NO) in rat retinal cells (RGCs) during synaptogenesis for variation phases of the pre- and postnatal developmental periods were investigated. The reinas from prenatal, lactaing, young, and adult rats were fixed in paraformaldehyde. The cryosections or paraformaldehyde-fixed ganglion cells purified from rat pupus were immunostained for constitutive isoforms of NOS(n and eNOS) and observed with confocal laser scanning microscope. Synthesis of No in the RGCs was achieved by in vitro stimulation with glutamete. The intracellular NO levels measured I real time using diaminofluorescein-2 diacetate, a, fluorescence indicator of NO. immunohistochemical analysis revaled nNOS and eNOSin vitro. Intracellular NO levels in cultured RGCs showed spontaneous fluctuation during a 20-min observation. The presence of both a non-specific NOS inhibitor, L-NAME, and a specific nNOS inhibitor, 7-NI, significantly inhibited the increase of intracellular NO 6 and 8 min after the introduction of L-arginine and glutamete to the medium. This study revealed that all constitutive NOS isoforms are expressed I RGCs and demonstrated that NO is produced by nNOS mainly through stimulation by glitamete in cultured RGCs. We addressed to examine if senescence may decrease the expression levels of tissue brain-derived neurotrophic factor (BDNF) and its receptor TrkB which are thought to support the survival of RGCs.
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Research Products
(13 results)
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[Publications] Chihiro Mayama, Yasuyuki Suzuki, Makoto Araie, Kyoko Ishida, Tsuji Akira, Tetsuya Yamamoto, Yoshiaki Kitazawa, Shigeo Funaki, Motohiro Shirakashi, Haruki Abe, Hidetoshi Tsukamoto, Koji Okada, Hiromu K. Mishima.: "Myopia and advanced-stage open-angle glaucoma"Ophthalmology. 109(11). 2072-2077 (2002)
Description
「研究成果報告書概要(欧文)」より
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