Study of phosphodiesterase in osteoblast

2002 Fiscal Year Final Research Report Summary

• Project/Area Number: 13470431
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Surgical dentistry
• Research Institution: Mie University
• Principal Investigator: MURATA Taku Mie University, Hospital, Research Associate, 医学部附属病院, 助手 (80242965)
• Project Period (FY): 2001 – 2002
• Keywords: osteoblast / phosphodiesterase

Research Abstract

In mouse calvaria osteoblastic MC3T3-E1 cells, total phosphodiesterases (PDEs) activities increased, but PDE3 and PDE4 activities did not increased. Diypridamole, PDE7, PDE8, PDE10 and PDE11 inhibitor, inhibited total PDE activity about 60%, and zapriment, PDE10 and PDE11 inhibitor, inhibites about 30 %, indicating the presence of PDE7, PDE8, PDE10 and PDE11 enzymes. PDE7, PDE8 and PDE10 mRNA were detected by RT-PCR in RNA from the cells. Dipyridamole inhibited alkaline phosphatase activity, Cbfa1/Pebp2 A 1, Osf2/Cbfa1, and osteocalcin expression. Dipyiramole, zaprinast, 8-Bromo-cAMP, and Folskolin inhibited the mineralization.
We found PDE3 and PDE4 activities in mouse fibroblastic cell line C3H10T1/2 clone 8 (10T1/2) cells. A PDE4 inhibitor did not stimulate ALP activity, but dramatically stimulated RA-induced ALP activity. A PDE3 inhibitor did not stimulate ALP activity, These data suggested PDE4 might be involed in regulation of osteoblast differentiation.
There were no abnormal finding in PDE3A and PDE3B knock-out mice bone. These data consisted with PDE3 inhibitor function in MC3T3-E1 and 10T1/2 cells.
These results suggest that PDE may be a critical rule of osteoblast differentiation.