2004 Fiscal Year Final Research Report Summary
Molecular analysis of the interaction between immune function and bone metabolisms in periodontitis
| Project/Area Number |
13470459
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Periodontal dentistry
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| Research Institution | Tokyo Medical and Dental University |
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Principal Investigator |
ISHIKAWA Isao Graduate School, Professor and chairman, 大学院・医歯学総合研究科, 教授 (10014151)
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| Co-Investigator(Kenkyū-buntansha) |
NOGUCHI Kazuyuki Graduate School, Research Associate, 大学院・医歯学総合研究科, 助手 (90218298)
NITTA Hiroshi Graduate School, Associate Professor, 大学院・医歯学総合研究科, 助教授 (70237767)
NAGASAWA Toshiyuki Graduate School, Research Associate, 大学院・医歯学総合研究科, 助手 (90262203)
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| Project Period (FY) |
2001 – 2004
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| Keywords | periodontitis / RANKL / OPG / IL-1 / T-cell |
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| Research Abstract |
Periodontopathic bacteria, immune responses and bone metabolisms were examined to clarify the interaction between immune responses and bone metabolisms in periodontitis. We have established the detection system for periodontopathic bacteria using real-time PCR analysis. Based on the system, we have found that periodontopathic bacteria were frequently detected in the surgically removed vessels from the cardio-vascular disease patients with periodontitis. In addition, monocytes from periodontitis patients were activated compared with healthy subjects. Serum IL-6 concentration was significantly elevated immediately after periodontal treatment. These results suggested that periodontitis might affect systemic health through bacteremia or production of inflammatory cytokines by activated monocytes. Bone metabolisms are regulated by osteoclasts and osteoblasts, and osteoclast differentiation is regulated by RANKL and OPG produced by osteoblasts. We have reported that RANKL expression was high in severe periodontitis lesion, and T-cells from periodontitis tissue expressed RANKL. Both periodontal ligament fibroblasts (PDL) and gingival fibroblasts produced OPG. IL-1 augmented RANKL expression in PDL five times higher than HGF, but OPG production was lower than HGF.
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Research Products
(27 results)
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[Journal Article] Biological activities of Bacteroides forsythus lipoproteins and their possible pathological roles in periodontal disease2004
Author(s)
Hasebe A, Yoshimura A, Into T, Kataoka H, Tanaka S, Arakawa S, Ishikura H, Golenbock DT, Sugaya T, Tsuchida N, Kawanami M, Hara Y, Shibata K
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Journal Title
Infect Immun 73・3
Pages: 1318-1325
Description
「研究成果報告書概要(和文)」より
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[Journal Article] Biological activities of Bacteroides forsythus lipoproteins and their possible pathological roles in periodontal disease2004
Author(s)
Hasebe A, Yoshimura A, Into T, Kataoka H, Tanaka S, Arakawa S, Ishikura H, Golenbock DT, Sugaya T, Tsuchida N, Kawanami M, Hara Y, Shibata K.
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Journal Title
Infect Immun 73・3
Pages: 1318-1325
Description
「研究成果報告書概要(欧文)」より
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