2002 Fiscal Year Final Research Report Summary

Human characteristic mechanism of enzyme induction and the prediction

Research Project

Project/Area Number	13470510
Research Category	Grant-in-Aid for Scientific Research (B)
Allocation Type	Single-year Grants
Section	一般
Research Field	応用薬理学・医療系薬学
Research Institution	Tohoku University
Principal Investigator	YAMAZOE Yasushi Tohoku Univ., Grad. Sch. of Pharm. Sci., Prof., 大学院・薬学研究科, 教授 (00112699)
Co-Investigator(Kenkyū-buntansha)	FURUKAWA Masayuki Otsuka Pharmaceutical Co., Ltd., Res., 研究員 MIYATA Masaaki Tohoku Univ., Grad. Sch. of Pharm. Sci., assist., 大学院・薬学研究科, 助手 (90239418) NAGATA Kiyoshi Tohoku Univ., Grad. Sch. of Pharm. Sci., Ass. Prof., 大学院・薬学研究科, 助教授 (80189133)
Project Period (FY)	2001 – 2002
Keywords	enzyme induction / stable expression / CYP3A4 / human drug metabolism / PXR
Research Abstract	As an estimation tool for human CYP3A4 induction, we have succeeded to make an adenovirus vecter system including CYP3A4 promoter and enhancer regions. The vecter was used to obtain the stably active clones in HepG2 cells. Among the clones selected, couple clones responded to typical chemical induces and showed high luciferase reporter activities. These clones are found to be candidates as effective tools for the quantitative prediction of inducing potential for human CYP3A4. We are currently evaluating the clones for the practical tools.

(6 results)