2003 Fiscal Year Final Research Report Summary
Analysis of thyroid transcription factor-2 (TTF-2) gene and cloning of causative genes for Cretinism
| Project/Area Number |
13470522
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Laboratory medicine
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| Research Institution | Dokkyo University School of Medicine |
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Principal Investigator |
IEIRI Tamio Dokkyo University School of Medicine, Professor, 医学部, 教授 (80049220)
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| Co-Investigator(Kenkyū-buntansha) |
HISHINUMA Akira Dokkyo University School of Medicine, Associate Professor, 医学部, 助教授 (40201727)
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| Project Period (FY) |
2001 – 2003
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| Keywords | Cretinism / thyroid dysgenesis / thyroid transcription factor-2 / thyroid / quantitative reverse transcription-polymerase chain reaction / tissue specificity / T1560 gene / T1560遺伝子 |
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| Research Abstract |
Thyroid dysgenesis is the most frequent cause of congenital hypothyroidism, but its molecular pathophysiology is largely unknown. Our hypothesis that. some genes downstream to thyroid transcription factor-2 (TTF-2) might be responsible for development of the thyroid prompted us to identify genes whose expression is stimulated by TTF-2. PCR product of cDNA clones obtained by a subtraction PCR method in TTF-2 expressing cell lines were screened with labeled cDNA by microarray analysis. We isolated 17 genes up-regulated by TTF-2, which were subsequently confirmed by quantitative RT-PCR. One of them is a novel gene designated T1560 that showed a highly thyroid-spesific expression pattern. Luciferase reporter assays showed that expression of all of the 14 genes tested was stimulated by both TTF-2 and TTF-1, another thyroid specific transcription factor. Our results have important implications for understanding normal thyroid development as well as the molecular defects underlying thyroid dysgenesis.
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Research Products
(12 results)
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[Publications] Baryshev, M., Sargsyan, E., Wallin G., Lejnieks A., Furudate, S., Hishinuma, A., Mkrtchian S.: "Unfolded protein response is involved in the pathology of human congenital hypothyroid goiter and rat non-goitrous congenital hypothyroidism"J.Mol Endocrinology. (in press).
Description
「研究成果報告書概要(和文)」より
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[Publications] Hishinuma, A., Ohyama, Y., Kuribayashi, T., Nagakubo, N., Namatame, T., Shibayama, K, Arisaka, O., MaLsuura, N., Iciri, T.: "Polymorphism of the polyalanine tract of thyroid transcription factor-2 gene in patients with thyroid dysgenesis."Eur.J.Endocrinol.. 145(4). 385-389 (2001)
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「研究成果報告書概要(欧文)」より
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[Publications] Kotani, T., Umeki, K., Kawano, J.I., Suganuma, T., Hishinuma, A., Ieiri, T., Harada, S.: "Partial iodide organification defect caused by a novel mutation of the : thyroid peroxidase gene in three siblings."Clin.Enodocrinol.. 59(2). 198-206 (2003)
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「研究成果報告書概要(欧文)」より
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[Publications] Baryshev, M., Sargsyan,.K, Wallin, G., Lejnieks, A., Furudate, S-I., Hishinuma, A., Mkrtchian, S.: "Unfolded protein response is involved in the pathology of human congenital hypothyroid goiter and rat non-goitrous congenital hypothyroidism."J.Mol Endocirinol. (in press).
Description
「研究成果報告書概要(欧文)」より
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