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2003 Fiscal Year Final Research Report Summary

Transgenermonal mutations in the children of the liquidators who worked for the Chernobyl nuclear power plant accident

Research Project

Project/Area Number 13480167
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field 環境影響評価(含放射線生物学)
Research InstitutionOsaka University

Principal Investigator

RYO Haruko  Osaka University, Graduate School of Medicine, Assistant Professor, 医学系研究科, 助手 (90301267)

Co-Investigator(Kenkyū-buntansha) NAKAJIMA Hiroo  Osaka University, Graduate School of Medicine, Assistant Professor, 医学系研究科, 助手 (20237275)
NOMURA Taisei  Osaka University, Graduate School of Medicine, Professor, 医学系研究科, 教授 (90089871)
Project Period (FY) 2001 – 2003
KeywordsChernobyl / radionuclides contamination / genetic effects / lymphocytes / microsatellite / low dose・low dose rate / germ cell mutation / human mutation
Research Abstract

To examine the transgenerational mutations by ionizing radiation at low dose and low dose rate in human, microsatellite length polymorphic mutations have been investigated in the F1 offspring of the liquidators who worked for the Chernobyl accident. Lymphocytes from peripheral blood were collected from the of exposed families (consisted of male liquidators living in Minsk and his partner and children) and from the unexposed families (consisted of fathers living in Minsk, uncontaminated area, with no history of liquidators and his partner and children whose age and sex were matched those of the children in exposed group) with the collaboration of Dr. Yelisseeva group. After the original lymphocytes were expanded, DNA was extracted and mutations were assayed. Final number of Fl offsprings was 62 and 85 for the exposed and unexposed control group, respectively. Twenty-one autosomal, one X linked and 21 Y linked microsarellites were used. For the autosomal microsatellite, mutation frequencies (/locus/gamete) were 0.64% (12/1858) and 0.80% (19/2362) in exposed and unexposed group, respectively. For the Y-linked microsatellites, mutation frequencies for the exposed and unexposed group were 0.44% (3/675) and 0.23% (2/835), respectively. This indicates that no difference of the mutation frequency between two group in the autosomal microsatellites. For the Y-linked microsatellites, tendency of higher mutation frequency has been seen in the exposed groups, though it is not significant. For the exposed group, 40 F1 children were from spermatogonial and 22 were from post spermatogonial exposure. The mutation frequency for the post spermatogonial was lower than that for the spermatogonial exposure.

  • Research Products

    (14 results)

All Other

All Publications (14 results)

  • [Publications] Nomura, T, et al.: "Transgenerational transmission of radiation- and chemically-induced tumors and congenital anomalies in mice : studies of their possible relationship to induced chromosomal and molecular changes."Cytogenet.Genome Res.. (in press). (2004)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Furitsu K, et al.: "Transgenerational mutations in the children of the liquidators who worked for the Chernobyl nuclear power plant accident."J.Radiat.Res.. 44(4). 420 (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nakajima, H. et al.: "Biodosimetry by detectiong γ-H2AX foci in human peripheral lymphocytes and mouse organ tissuee after ^<137>Cs γ-ray irradiation."J.Radiat.Res.. 44(4). 406 (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Wani, K.M.Y., et al.: "Genetic alterations in the coding region of bak gene in uterine cervical cancer."Brit.J.Cancer. 88(10). 1584-1586 (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nomura, T.: "Transgenerational carcinogenesis ; induction and transmission of genetic alterations and mechanism of carcinogenesis."Mut.Res.. 511(2-3). 425-432 (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Baskar, R. et al.: "Spontaneous and radiation-induced tumorgenesis in p53-deficient mice."Int.Cong.Series.. 1236. 115-189 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nomura, T., et al.: "Radiobiology and Bio-Medical Reseach ; A novel SCID biotechnology for biomedical studies in human."Narosa Pub.House, New Delhi. 208 (2004)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nomura, T. et al.: "Transgenerational transmission of radiation-and congenital anomalies in mice : studies of the possible relationship to induced chromosomal and molecular changes."Cytogenet.Genome Res. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Furitu, K. et al.: "Transgenerational mutations in the children of the liquidators who worked for the Chernobyl nuclear power plant accident"J.Radiat.Res.. 44(4). 420 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nakajima, H. et al.: "Biodosimetry by detecting γ-H2AX foci in human peripherial lymphocytes and mouse organ after <137>^Cs irradiation."J.Radiat.Res.. 88(10). 1584-1586 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Wani, K.M.Y.et al.: "Genetic alterations in the coding region of bak gene in uterine cervical cancer."Brit.J.Cancer.. 88(10). 425-432 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nomura, T.: "Transgenerational carcinogenesis : induction and transmission of genetic alterations and mechanism of carcinogenesis."Mut.Res.. 544(2-3). 425-432 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Baskar, et al.: "Spontaneous and radiation-induced tumorgenesis in p53-deficient mice."Int.Cong, Series.. 1236. 115-118 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nomura, T. et al.: "Narosa Pub.House (New Delhi)"A novel SCID biotechnology for biomedical studies in human..

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2005-04-19  

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