2002 Fiscal Year Final Research Report Summary
Analysis of genetic interactions of DNA repair pathways
| Project/Area Number |
13480228
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Molecular biology
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| Research Institution | KYOTO UNIVERSITY |
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Principal Investigator |
TAKEDA Shunichi Kyoto University, Faculty of Medicine, Professor, 医学研究科, 教授 (60188191)
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| Co-Investigator(Kenkyū-buntansha) |
TAKATA Minoru Kawasaki Medical School, Professor, 教授 (30281728)
NAKAYAMA Tatsuo Miyazaki Medical College, Professor, 教授 (60031712)
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| Project Period (FY) |
2001 – 2002
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| Keywords | DNA damage / DNA replication / Translesion DNA synthesis / Homologous DNA recombinatior / DNA polymerase / DT40 / Sister chromatid exchange |
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| Research Abstract |
During each cell cycle replication forks inevitably stall at damaged DNA. The ability to overcome DNA lesions is an essential feature of the replication machinery. A large variety of specialized polymerases have recently been discovered that enable cells to replicate past various forms of damage. Alternatively homologous recombination can be used to restart DNA replication across the lesion. Genetic studies have shed light on the impact of these two post replication repair pathways in bacteria and yeast. In vertebrates, however, a genetic approach to study post replication repair has been compromised because most of the genes involved appear to be essential for embryonic development. We have taken advantage of the chicken cell line DT40 to perform a genetic analysis of translesion synthesis and homologous recombination and to characterize genetic interactions between these two pathways in vertebrates. In this article we aim to summarize our current understanding of post replication repair in DT40 in the perspective of results from other model systems.
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[Publications] M.Takata, M.S.Sasaki, E.Sonoda, T.Fukushima, J.Albala, S.Swagemakers, R.Kanaar, L.H.Thompson, S.Takeda: "Targeted disruption of the RAD51B, a member of RAD51-related gene family, impairs homologous recombination and repair of crosslink DNA damages"Mol.Cell.Biol.. 20. 6476-6482 (2000)
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「研究成果報告書概要(和文)」より
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[Publications] Okada, T., Sonoda, E., Yamashita, Y. M., Koyoshi, S., Tateishi, S., Yamaizumi, M., Takata, M., Ogawa, O., and Takeda, S. ': "Involvement of Vertebrate Pol k in Rad18-independent Postreplication Repair of UV damage"J Biol. Chem.. 277. 48690-48695 (2002)
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「研究成果報告書概要(欧文)」より
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[Publications] Yamashita, Y. M., Okada, T., Matsusaka, T, SonodaI, E., Cho, Z., Araki, K., Tateishi, S., Yamaizumi, M., and Takeda, S: "RAD18 and RAD54 cooperatively contribute to maintenance of genomic stability in vertebrate cells"EMBO J. 21. 5558-5566 (2002)
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[Publications] Tauchi H, Kobayashi J, Morishima K, van Gent DC, Shiraishi T, Verkaik NS, vanHeems D, Ito E, Nakamura A, Sonoda E, Takata M. Takeda S, Matsuura S, Komatsu K: "Nbs1 is essential for DNA repair by homologous recombination in higher vertebrate cells"Nature. 420. 93-98 (2002)
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「研究成果報告書概要(欧文)」より
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[Publications] Takata, M., Tachiiri, S., Fujimori, A., Thompson, L. H., Miki, Y., Hiraoka, M., Takeda S., and Yamazoe, M.: "Conserved domains in the chicken homologue of BRCA2"Oncogene. 21. 1130-1134 (2002)
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「研究成果報告書概要(欧文)」より
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