• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

2003 Fiscal Year Final Research Report Summary

Structural basis for regulation of chromatin structure by DNA methylation

Research Project

Project/Area Number 13480230
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Molecular biology
Research InstitutionYokohama City University

Principal Investigator

SHIRAKAWA Masahiro  Yokohama City University, Graduate School of Integrated Science, Professer (00202119)

Project Period (FY) 2001 – 2003
KeywordsDNA methylation / ubiquitination / DNA repair / NMR
Research Abstract

The purpose of this study was to clarify the regulation of chromatin structures mediated by genome DNA methylation, and to investigate post-translational modifications of nuclear proteins by ubiquitin-like modifier proteins. We proceeded structural and functional analyses of the methylated DNA binding domain (MBD) of MBD1, the ubiquitin-like domain of human HR23B, and the ubiquitin-associated (UBA) domain of Saccharomyces cerevisae Rad23 and Lys 48- or Lys 63-linked di- or tetra-ubiquitin.
We analyzed the dynamic property of the complex between MBD1 MBD and a methylated DNA by using NMR, SPR and isothermal calorimetry. We have determined solution structure of ubiquitin-like domain of human HR23B in complex with a ubiquitin interacting motif of proteasomal S5a subunit by NMR. We also determined solution structure of the complex between the UBA domain of yeast DSK2 and ubiquitin. In addition, we showed that show the difference in structural properties of Lys 48- or Lys 63-linked ubiquitin chains by isotope-aided NMR and small angle X-ray scattering.

  • Research Products

    (8 results)

All 2004 2003

All Journal Article (8 results)

  • [Journal Article] Structure of the ubiquitin-interacting motif of S5a bound to the ubiquitin-like domain of HR23B.2004

    • Author(s)
      Fujiwara,K., Tenno,T., Sugasawa,K., Jee,J.-G., Ohki,I., Kojima,C., Tochio,H., Hiroaki,H., Hanaoka,F., shirakawa,M.
    • Journal Title

      Journal of Biological Chemistry 279

      Pages: 4760-4767

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Structure of the ubiquitin-interacting motif of S5a bound to the ubiquitin-like domain of HR23B2004

    • Author(s)
      Fujiwara, K., Tenno, T., Sugasawa, K., Jee, J.-G., Ohki, I., Kojima, C., Tochio, H., Hiroaki, H., Hanaoka, F., Shirakawa, M.
    • Journal Title

      Journal of Biological Chemistry 279

      Pages: 4760-4767

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] The structure and binding mode of interleukin-18.2003

    • Author(s)
      Kato,Z, Jee,J.-G., Shikano,H., Mishima,M., Ohki,I., Ohnishi,H., Li,A., Hashimoto,K., Matsukuma,E., Omoya,K., Yamamoto,Y., Yoneda,T., Hara,T., Kondo,N., Shirakawa,M.
    • Journal Title

      Nature Structural Biology 10

      Pages: 966-971

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Structure of the male determinant factor for Brassica self-incompatibility.2003

    • Author(s)
      Mishima,M., Takayama,S., Saski,K., Jee,J.G., Kojima,C., Iogai,A., Shirakawa,M.
    • Journal Title

      Journal of Biological Chemistry 278

      Pages: 36389-36395

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Methylated DNA-binding domain 1 and methylpurine-DNA glycosylase link transcriptional repression and DNA repair in chromatin.2003

    • Author(s)
      Watanabe,S., Ichimura,T., Fujita,N., Tsuruzoe,S., Ohki,I., Shirakawa,M., Kawasuji,M., Nakao,M.
    • Journal Title

      Proceedings of Natural Academy of Sciences USA 100

      Pages: 12859-12864

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] The structure and binding modeof interleukin-182003

    • Author(s)
      Kato, Z, Jee, J.-G., Shikano, H., Mishima, M., Ohki, I., Ohnishi, H., Li, A., Hashimoto, K., Matsukuma, E., Omoya, K., Yamamoto, Y., Yoneda, T., Hara, T., Kondo, N., Shirakawa, M.
    • Journal Title

      Nature Structural Biology 10

      Pages: 966-971

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Structure of the male determinant factor for Brassica self-incompatibility2003

    • Author(s)
      Mishima, M., Takayama, S., Sasaki, K., Jee, J.G., Kojima, C., Isogai, A., Shirakawa, M.
    • Journal Title

      Journal of Biological Chemistry 278

      Pages: 36389-36395

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Methylated DNA-binding domain 1 and methylpurine-DNA glycosylase link transcriptional repression and DNA repair in chromatin2003

    • Author(s)
      Watanabe, S., Ichimura, T., Fujita, N., Tsuruzoe, S., Ohki, I., Shirakawa, M., Kawasuji, M., Nakao, M.
    • Journal Title

      Proceedings of Natural Academy of Sciences USA 100

      Pages: 12859-12864

    • Description
      「研究成果報告書概要(欧文)」より

URL: 

Published: 2011-06-18  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi