2003 Fiscal Year Final Research Report Summary
Genetic Variation and Reproductive Biology of fragmented Dipterocarps in Thailand
| Project/Area Number |
13490022
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
広領域
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| Research Institution | Kyushu University |
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Principal Investigator |
SZMIDT Alfred Kyushu University, Grad.School of Sciences, Associate Prof., 大学院・理学研究院, 助教授 (70325497)
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| Co-Investigator(Kenkyū-buntansha) |
INOMATA Nobuyuki Kyushu University, Grad.School of Sciences, Assistant Prof, 大学院・理学研究院, 助手 (20301335)
YAMASAKI Tsuneyuki Kyushu University, Grad.School of Sciences, Prof emeritus, 大学院・理学研究院, (名誉教授) (10108649)
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| Project Period (FY) |
2001 – 2003
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| Keywords | Dipterocarps / genetic variation / population structure / evolution / Tropical forest |
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| Research Abstract |
Our research activities were focused on the following three themes.
1.To determine the importance of apomixis in the reproductive biology of dipterocarps we have carried out analysis of nuclear microsatellite variation in a population of Dipterocarpus alatus. The results provided first genetic evidence for the occurrence of apomixis in the species and indicated that it can reduce the levels of genetic variation within populations.
2.To determine the levels and patterns of genetic polymorphism in various members of the Dipterocarpaceae family we have studied nucleotide polymorphism in two nuclear genes(GapC and Pgi) in populations of Shorea acuminata, S.curtisii S.parvifolia and S.leprosula. The study suggested recent speciation of the family but compared to other tree species considerable population differentiation. Furthermore, it provided first genetic evidence for the occurrence of a widespread inter-specific hybridization in dipterocarps.
3.To determine phylogenetic position of endemic dipterocarp species native to Sri Lanka we have constructed molecular phylogeny for 74 species of Dipterocarpaceae based on three chloroplast DNA sequences. Results of this work revealed divergent character of Sri Lankan species, which is probably due to their prolonged isolation from the other Asian members of the family.
In the course of the project, three MSc and two BSc programs were completed.
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Research Products
(32 results)
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[Publications] Parani, M., Rajesh, K., Lakshmi, M., Parducci, L., Szmidt, A.E., Parida, A.: "Species identification in seven small millet species using PCR-RFLP of trn^S-psb^C gene region."Genome. 44(3). 495-499 (2001)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Ribeiro, M.M., Mariette, S., Vendramin, G.G., Szmidt, A.E., Plomion, C., Kremer, A.: "Comparison of genetic diversity estimates within and among populations of maritime pine using cpSSR and AFLP data."Molecular Ecology. 11. 869-877 (2002)
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[Publications] Wang, X-R., Chhatre, V.E., Nilsson, M-C., Song, W., Zackrisson, O., Szmidt, A.E.: "Island population structure of Norway spruce(Picea abies) in northern Sweden"International Journal of Plant Sciences. 164. 711-717 (2003)
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[Publications] Ishiyama, H., Kado, T., Iwasaki, M., Ab Shukor, N., Szmidt, A.E., Yamazaki, T.: "Nucleotide variation in the GapC region of four species of Shorea and its putative hybrids."Tropics. 13(2). 89-99 (2003)
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[Publications] Sirikantaramas, S., Sugioka, N., Lee, S-S., Ibrahim, F.H., Lee, H-S., Szmidt, A.E., Yamazaki, T.: "Diversity of ectomycorrhizal fungi associated with Dipterocarpaceae in Malaysia."Tropics. 13(2). 69-77 (2003)
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「研究成果報告書概要(欧文)」より
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