2002 Fiscal Year Annual Research Report

日本産魚類のミトコンドリアゲノム・データベースの構築

Research Project

Project/Area Number	13556028
Research Institution	The University of Tokyo
Principal Investigator	西田睦東京大学, 海洋研究所, 教授 (90136896)
Co-Investigator(Kenkyū-buntansha)	宮正樹千葉県立中央博物館, 動物学研究科, 上席研究員 (30250137) 松浦啓一国立科学博物館, 動物研究部, 動物第二研究室室長(研究職) (70141984)
Keywords	魚類 / ミトコンドリアゲノム / mtDNA / 塩基配列 / データベース
Research Abstract	水域生態系の重要構成要素である魚類は,資源生物としてもきわめて重要なグループである。この魚類には,多様な系統に属する2万5千もの種が存在しており,卵や稚仔魚を含むあらゆるタイプの試料の種の正確な識別と分類,遺伝的多様性の把握,種内集団(系群)の峻別,分子系統分析などに,各種のDNA塩基配列情報が重要かつ不可欠になりつつあり,急速に増大するDNA情報を使いやすい形で整理・蓄積することが強く求められている。そこで本研究では,種々の面から有用性の高い情報源であることが明らかになっているミトコンドリアゲノムを取り上げ,「魚類のミトコンドリアゲノム・データベース」を構築することを目指す。この目標達成のために,プロジェクト2年目にあたる今年度は,1)国内および国外からさまざまな系統を代表する約100種の貴重な標本試料を新たに入手し,2)確立したロングPCRと魚類ユニバーサルプライマーを活用する方法を用いて,それらのミトコンドリアゲノムの全塩基配列を決定した。加えて,同ゲノムの部分塩基配列を約200種について得た。3)魚体標本は引き続き,国立科学博物館ないし千葉県中央博物館への登録を進めた。そして,4)前年度に設計した基本デザインに従って,「魚類のミトコンドリアゲノム・データベース」の暫定版を作成した。プロジェクト最終年度の次年度には,さらに標本とDNAデータの収集を行ってこのデータベースの充実を図るとともに,データベースの試用を通じてその完成を目指す。

Research Products

(14 results)

All Other

All Publications (14 results)

[Publications] Mabuchi, Kohji: "Genetic comparison of two Choerodon (Osteichthyes : Perciformes : Labridae) color-types from the southern coasts of Japan"Species Diversity. 7. 387-392 (2002)
[Publications] Yamauchi, Mitsugu M.: "Complete mitochondrial DNA sequence of the Japanese spiny lobster, Panulirus japonicus (Crustacea : Decapoda)"Gene. 295. 89-96 (2002)
[Publications] Machida, Ryuji J.: "Complete mitochondrial DNA sequence of Tigriopus japonicus (Crustacea : Copepoda)"Marine Biotechnology. 4. 406-417 (2002)
[Publications] Kitaura, Jun: "Molecular phylogeny of grapsoid of and ocypodoid crabs with special reference to the genera Metaplax and Macrophthalmus"Journal of Crustacean Biology. 22. 682-693 (2002)
[Publications] Kitaura, Jun: "Genetic and behavioral diversity in the Macrophthalmus japonicus species complex (Crustacea : Brachyura : Ocypodidae)"Marine Biology. 14. 1-8 (2002)
[Publications] Iguchi, Kei'ichiro: "Genetic structure of land-locked ayu within the Biwa Lake system"Fisheries Science. 68. 138-143 (2002)
[Publications] Watanabe, Katsutoshi: "Genetic relationship and origin of the two geographic populations of the freshwater threespine stickleback, 'Hariyo'"Zoological Science. 20. 265-274 (2003)
[Publications] Inoue, Jun G.: "Basal actinopterygian relationships : A mitogenomic perspective on the phylogeny of the "ancient fish.""Molecular Phylogenetics and Evolution. 26. 110-120 (2003)
[Publications] Miya, Masaki: "Major Patterns of higher teleostean phylogenies : A new perspective based on 100 complete mitochondrial DNA sequences"Molecular Phylogenetics and Evolution. 26. 121-138 (2003)
[Publications] Saitoh, Kenji: "Mitochondrial genomics of ostariophysan fish : Perspectives on phylogeny and biogeography"Journal of Molecular Evolution. 56. 1-9 (2003)
[Publications] Yamazaki, Yuji: "Mitochindrial DNA sequence divergence between two cryptic species of genus Lethenteron, with reference to an improved identification technique"Journal of Fish Biology. (in press). (2003)
[Publications] Watanabe, Katsutoshi: "Genetic population structure of Japanese bagrid catfishes"Ichthyological Research. (in press). (2003)
[Publications] Iguchi, Kei'ichiro: "Morphological and Genetic analysis of fish of a Carassius complex (Cyprinidae) in Lake Kasumiganra with reference to the taxonomic status of all-female triploid morph"Biological Journal of Linnean Society. (in press). (2003)
[Publications] Ishiguro, Naoya: "Basal euteleostean relationships : A mitogenomic perspective on the phylogenetic reality of the "Protacanthopterygii.""Molecular Phylogenetics and Evolution. (in press). (2003)

2002 Fiscal Year Annual Research Report

日本産魚類のミトコンドリアゲノム・データベースの構築

Principal Investigator

西田 睦 東京大学, 海洋研究所, 教授 (90136896)

Research Products

[Publications] Mabuchi, Kohji: "Genetic comparison of two Choerodon (Osteichthyes : Perciformes : Labridae) color-types from the southern coasts of Japan"Species Diversity. 7. 387-392 (2002)

[Publications] Yamauchi, Mitsugu M.: "Complete mitochondrial DNA sequence of the Japanese spiny lobster, Panulirus japonicus (Crustacea : Decapoda)"Gene. 295. 89-96 (2002)

[Publications] Machida, Ryuji J.: "Complete mitochondrial DNA sequence of Tigriopus japonicus (Crustacea : Copepoda)"Marine Biotechnology. 4. 406-417 (2002)

[Publications] Kitaura, Jun: "Molecular phylogeny of grapsoid of and ocypodoid crabs with special reference to the genera Metaplax and Macrophthalmus"Journal of Crustacean Biology. 22. 682-693 (2002)

[Publications] Kitaura, Jun: "Genetic and behavioral diversity in the Macrophthalmus japonicus species complex (Crustacea : Brachyura : Ocypodidae)"Marine Biology. 14. 1-8 (2002)

[Publications] Iguchi, Kei'ichiro: "Genetic structure of land-locked ayu within the Biwa Lake system"Fisheries Science. 68. 138-143 (2002)

[Publications] Watanabe, Katsutoshi: "Genetic relationship and origin of the two geographic populations of the freshwater threespine stickleback, 'Hariyo'"Zoological Science. 20. 265-274 (2003)

[Publications] Inoue, Jun G.: "Basal actinopterygian relationships : A mitogenomic perspective on the phylogeny of the "ancient fish.""Molecular Phylogenetics and Evolution. 26. 110-120 (2003)

[Publications] Miya, Masaki: "Major Patterns of higher teleostean phylogenies : A new perspective based on 100 complete mitochondrial DNA sequences"Molecular Phylogenetics and Evolution. 26. 121-138 (2003)

[Publications] Saitoh, Kenji: "Mitochondrial genomics of ostariophysan fish : Perspectives on phylogeny and biogeography"Journal of Molecular Evolution. 56. 1-9 (2003)

[Publications] Yamazaki, Yuji: "Mitochindrial DNA sequence divergence between two cryptic species of genus Lethenteron, with reference to an improved identification technique"Journal of Fish Biology. (in press). (2003)

[Publications] Watanabe, Katsutoshi: "Genetic population structure of Japanese bagrid catfishes"Ichthyological Research. (in press). (2003)

[Publications] Iguchi, Kei'ichiro: "Morphological and Genetic analysis of fish of a Carassius complex (Cyprinidae) in Lake Kasumiganra with reference to the taxonomic status of all-female triploid morph"Biological Journal of Linnean Society. (in press). (2003)

[Publications] Ishiguro, Naoya: "Basal euteleostean relationships : A mitogenomic perspective on the phylogenetic reality of the "Protacanthopterygii.""Molecular Phylogenetics and Evolution. (in press). (2003)

西田睦東京大学, 海洋研究所, 教授 (90136896)