2003 Fiscal Year Annual Research Report

日本産魚類のミトコンドリアゲノム・データベースの構築

Research Project

Project/Area Number	13556028
Research Institution	The University of Tokyo
Principal Investigator	西田睦東京大学, 海洋研究所, 教授 (90136896)
Co-Investigator(Kenkyū-buntansha)	宮正樹千葉県立中央博物館, 動物学研究科, 上席研究員 (30250137) 松浦啓一国立科学博物館, 動物研究部, 動物第二研究室室長 (70141984)
Keywords	魚類 / ミトコンドリアゲノム / mtDNA / 塩基配列 / データベース
Research Abstract	水域生態系の重要構成要素である魚類は,資源生物としてもきわめて重要なグループである。この魚類には,多様な系統に属する2万5千もの種が存在しており,卵や稚仔魚を含むあらゆるタイプの試料の種の正確な識別と分類,遺伝的多様性の把握,種内集団(系群)の峻別,分子系統分析などに,各種のDNA塩基配列情報が重要かつ不可欠になりつつあり,急速に増大するDNA情報を使いやすい形で整理・蓄積することが強く求められている。そこで本研究では,種々の面から有用性の高い情報源であることが明らかになっているミトコンドリアゲノムを取り上げ,「魚類のミトコンドリアゲノム・データベース」を構築することを目指した。プロジェクト3年間で,1)国内および国外からさまざまな系統を代表する約数百種の貴重な標本試料を新たに入手し,2)確立したロングPCRと魚類ユニバーサルプライマーを活用する方法を用いて約200種のミトコンドリアゲノムの全塩基配列を決定した。3)加えて,同ゲノムの部分塩基配列を約400種について得た。4)魚体標本の多くは,国立科学博物館ないし千葉県中央博物館へ登録した。そして5)最終目標である「魚類のミトコンドリアゲノム・データベース」を完成させ,インターネット上に公開した。URLはhttp://mitofish.ori.u-tokyo.ac.jp/であり,ミラーサイトが国立科学物館にある。本データベースは国際的使用が可能な英語仕様である。機能としては,国際的DNAデータベースにミトコンドリアゲノムの全塩基配列データが登録されているすべての魚類の情報が検索できる。また,このデータベースに対して,任意の塩基配列の相同性検索ができるので,種や魚類グループの識別に有用である。今後、さらに充実を図る予定であるが,データの増大に伴ってその有用性は飛躍的に大きくなるはずである。

Research Products

(20 results)

All Other

All Publications (20 results)

[Publications] Yamanoue, Yusuke: "Mitochondrial genomes and phylogeny of the ocean sunfishes (Tetraodontiformes : Molidae)."Ichthyological Research. (in press). (2004)
[Publications] Mabuchi, Kohji: "Gene rearrangements and evolution of tRNA pseudogenes in the mitochondrial DNA of the parrotfishes (Teleostei : Perciformes : Scaridae)."Journal of Molecular Evolution. (in press). (2004)
[Publications] Mabuchi, Kohji: "Molecular phylogeny of the antitropical genus Pseudolabrus (Perciformes : Labridae) : evidence for a Southern Hemisphere origin."Molecular Phylogenetics and Evolution. (in press). (2004)
[Publications] Kumazawa, Yoshinori: "Tempo of mitochndrial gene evolution : Can mitochondrial DNA be used to date Old divergence?"Proceedings ICES8. (in press). (2004)
[Publications] Mabuchi, Kohji: "Genetic differentiations between two color-morphs of Apogon taeniophorus from Southern Japan."Ichthyological Research. (in press). (2004)
[Publications] Inoue, Jun G.: "Mitogenomic evidence for the monophyly of elopomorph fishes (Teleostei) and the evolutionary origin of the leptocephalus larva."Molecular Phylogenetics and Evolution. (in press). (2004)
[Publications] Ikejima, Kou: "Molecular phylogeny and possible scenario of ponyfish (Perciformes : Leiognathidae) evolution."Molecular Phylogenetics and Evolution. (in press). (2004)
[Publications] Inoue, Jun, G.: "Evolution of the deep-sea gulper eel mitochondrial genomes : large-scale gene rearrangements originated within the eels."Molecular Biology and Evolution. (in press). (2004)
[Publications] Mukai, Takahiko: "Genetic differentiation of the brackish water goby, Eutaeniichthys gilli (Perciformes, Gobiidae), between the Japanese and the Ryukyu Archipelagos."Biogeography. 5. 49-53 (2003)
[Publications] Mabuchi, Kohji: "Genetic comparison of two color-morphs of Apogon properuptus from southern Japan"Ichthyological Research. 50. 293-296 (2003)
[Publications] Iguchi, Kei'ichiro: "Morphological and genetic analysis of fish of a Carassius complex (Cyprinidae) in Lake Kasumigaura with reference to the taxonomic status of two all -female triploid morphs."Biological Journal of Linnean Society. 79. 351-357 (2003)
[Publications] 向井貴彦: "日本産ドンコにおけるミトコンドリアDNAの系統と関東地方への人為移植の分子的証拠"魚類学雑誌. 50. 71-76 (2003)
[Publications] Yamazaki, Yuji: "Mitochondrial DNA sequence divergence between two cryptic species of genus Lethenteron, with reference to an improved identification technique."Journal of Fish Biology. 62. 591-619 (2003)
[Publications] Watanabe, Katsutoshi: "Genetic population structure of Japanese bagrid catfishes."Ichthyological Research. 50. 140-148 (2003)
[Publications] Ishiguro, Naoya: "Basal euteleostean relationships : A mitogenomic perspective on the phylogenetic reality of the "Protacanthopterygii"."Molecular Phylogenetics and Evolution. 27. 476-488 (2003)
[Publications] Saitoh, Kenji: "Mitochondrial genomics of ostariophysan fishes : perspectives on phylogeny and biogeography"Journal of Molecular Evolution. 56. 464-472 (2003)
[Publications] Watanabe, Katsutoshi: "Genetic relationships and origin of the two geographic populations of the freshwater threespine stickleback, 'Hariyo'."Zoological Science. 20. 265-274 (2003)
[Publications] Inoue, Jun G.: "Basal actinopterygian elationships : a mitogenomic perspective on the phylogeny of the "ancient fish"."Molecular Phylogenetics and Evolution. 26. 110-120 (2003)
[Publications] Miya, Masaki: "Major patterns of higher teleostean phylogenies : a new perspective based on 100 complete mitochondrial DNA sequences."Molecular Phylogenetics and Evolution. 26. 121-138 (2003)
[Publications] 前川光司(編著): "サケ・マスの生態と進化"文一総合出版,東京(印刷中). (2004)

2003 Fiscal Year Annual Research Report

日本産魚類のミトコンドリアゲノム・データベースの構築

Principal Investigator

西田 睦 東京大学, 海洋研究所, 教授 (90136896)

Research Products

[Publications] Yamanoue, Yusuke: "Mitochondrial genomes and phylogeny of the ocean sunfishes (Tetraodontiformes : Molidae)."Ichthyological Research. (in press). (2004)

[Publications] Mabuchi, Kohji: "Gene rearrangements and evolution of tRNA pseudogenes in the mitochondrial DNA of the parrotfishes (Teleostei : Perciformes : Scaridae)."Journal of Molecular Evolution. (in press). (2004)

[Publications] Mabuchi, Kohji: "Molecular phylogeny of the antitropical genus Pseudolabrus (Perciformes : Labridae) : evidence for a Southern Hemisphere origin."Molecular Phylogenetics and Evolution. (in press). (2004)

[Publications] Kumazawa, Yoshinori: "Tempo of mitochndrial gene evolution : Can mitochondrial DNA be used to date Old divergence?"Proceedings ICES8. (in press). (2004)

[Publications] Mabuchi, Kohji: "Genetic differentiations between two color-morphs of Apogon taeniophorus from Southern Japan."Ichthyological Research. (in press). (2004)

[Publications] Inoue, Jun G.: "Mitogenomic evidence for the monophyly of elopomorph fishes (Teleostei) and the evolutionary origin of the leptocephalus larva."Molecular Phylogenetics and Evolution. (in press). (2004)

[Publications] Ikejima, Kou: "Molecular phylogeny and possible scenario of ponyfish (Perciformes : Leiognathidae) evolution."Molecular Phylogenetics and Evolution. (in press). (2004)

[Publications] Inoue, Jun, G.: "Evolution of the deep-sea gulper eel mitochondrial genomes : large-scale gene rearrangements originated within the eels."Molecular Biology and Evolution. (in press). (2004)

[Publications] Mukai, Takahiko: "Genetic differentiation of the brackish water goby, Eutaeniichthys gilli (Perciformes, Gobiidae), between the Japanese and the Ryukyu Archipelagos."Biogeography. 5. 49-53 (2003)

[Publications] Mabuchi, Kohji: "Genetic comparison of two color-morphs of Apogon properuptus from southern Japan"Ichthyological Research. 50. 293-296 (2003)

[Publications] Iguchi, Kei'ichiro: "Morphological and genetic analysis of fish of a Carassius complex (Cyprinidae) in Lake Kasumigaura with reference to the taxonomic status of two all -female triploid morphs."Biological Journal of Linnean Society. 79. 351-357 (2003)

[Publications] 向井貴彦: "日本産ドンコにおけるミトコンドリアDNAの系統と関東地方への人為移植の分子的証拠"魚類学雑誌. 50. 71-76 (2003)

[Publications] Yamazaki, Yuji: "Mitochondrial DNA sequence divergence between two cryptic species of genus Lethenteron, with reference to an improved identification technique."Journal of Fish Biology. 62. 591-619 (2003)

[Publications] Watanabe, Katsutoshi: "Genetic population structure of Japanese bagrid catfishes."Ichthyological Research. 50. 140-148 (2003)

[Publications] Ishiguro, Naoya: "Basal euteleostean relationships : A mitogenomic perspective on the phylogenetic reality of the "Protacanthopterygii"."Molecular Phylogenetics and Evolution. 27. 476-488 (2003)

[Publications] Saitoh, Kenji: "Mitochondrial genomics of ostariophysan fishes : perspectives on phylogeny and biogeography"Journal of Molecular Evolution. 56. 464-472 (2003)

[Publications] Watanabe, Katsutoshi: "Genetic relationships and origin of the two geographic populations of the freshwater threespine stickleback, 'Hariyo'."Zoological Science. 20. 265-274 (2003)

[Publications] Inoue, Jun G.: "Basal actinopterygian elationships : a mitogenomic perspective on the phylogeny of the "ancient fish"."Molecular Phylogenetics and Evolution. 26. 110-120 (2003)

[Publications] Miya, Masaki: "Major patterns of higher teleostean phylogenies : a new perspective based on 100 complete mitochondrial DNA sequences."Molecular Phylogenetics and Evolution. 26. 121-138 (2003)

[Publications] 前川光司(編著): "サケ・マスの生態と進化"文一総合出版,東京(印刷中). (2004)

西田睦東京大学, 海洋研究所, 教授 (90136896)