| Project/Area Number |
13557141
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | Keio University |
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Principal Investigator |
YOSHIMURA Yasunori School of Medicine, Keio University Department of Obstetrics and Gynecology Professor, 医学部, 教授 (10129736)
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| Co-Investigator(Kenkyū-buntansha) |
MATSUI Minoru The Institute of Medical Science, The University of Tokyo instructor, 医科学研究所・基礎医科学部門, 助手 (50282611)
SHIMIZU Aki School of Medicine, Keio University Department of Obstetrics and Gynecology instructor, 医学部, 助手 (60306826)
MARUYAMA Tetsuo School of Medicine, Keio University Department of Obstetrics and Gynecology Assistant Professor, 医学部, 専任講師 (10209702)
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| Project Period (FY) |
2001 – 2002
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| Keywords | mouse / calcium binding protein D-9K / uterus / Cre-loxP / transgenic mouse / implantation |
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| Research Abstract |
The purpose of this study is to develop the animal model whose fertility can be controlled through modification of implantation-associated genes. We have been trying to employ two different approaches: 1) introduction of DNA or viral vectors harboring the gene of interest into the uterine cavity, 2) generation of genetically engineered mice whose implantation-related genes can be modulated spatially and temporally. Firstly, we have cloned calcium binding protein D-9K (Cabp9K) gene, which has been implicated in mouse embryo implantation, and generated its mammalian expression vectors as well as antisense oligonucleotides (AS-ODN) against Cabp9K. We also have been trying to establish the optimal condition of introducing the plasmid vectors into the mouse uterine cavity using reporter vectors harboring lacZ or GFP gene. Besides the development of the gene delivery system, we have successfully generated transgenic mice in which the expression of Cre recombinase can be spatially and temporally regulated in the uterus. To evaluate conditional DNA deletion, crossing the transgenic mice with the cActXstopXLacZ reporter mice is now underway.
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