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2002 Fiscal Year Final Research Report Summary

Functional Analyses of Environmental Signal Recognition and Development of Plant-pathogen.

Research Project

Project/Area Number 13660343
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied molecular and cellular biology
Research InstitutionRIKEN Institute

Principal Investigator

KAMAKURA Takashi  RIKEN Institute, Microbial Toxicology Lab., Senior Researcher, 化学遺伝学研究室, 先任研究員 (70177559)

Project Period (FY) 2001 – 2002
KeywordsMagnaporthe grisea / Appressorium formation / Chitin binditiR protein / NCS1 / pH stress / Plant pathogenic fungi / Development of infection specific organ
Research Abstract

In this research project, we have analyzed the gene function of CBP1 which may play an important role for| induction of the development of appressorium of rice blast pathogen Magnaporthe grisea. We estimated the promoter control region of the gene by using a reporter gene EGFP and succeeded to encompass the critical control region within less than one hundred base pairs. Cellular localization and function of the product of site-directed mutant and reporter-fusion protein were analyzed to find the importance of remarkable domains of the CBP1-product such as two chitin-binding domain, Ser/Thr-rich region, and C-terminufc. Especially, the C-terminus of this gene product seemed to be important to sort the gene product to the appropriate location of the cell.
We have also generated a gene disruptant of MgNCS1 which could code a homolog of Neuronal Calcium Sensor 1 (NCS1) protein. NCS1 is highly conserved among most of eukaryotic organisms from yeast to human. By analyzing the phenotype of MgNCS1 disruptant, we found the MgNCS1 is required for the resistance against the high Calcium concentration and low pH. Relationship between NCS1 family and pH stress has firstly found in this research project.

  • Research Products

    (6 results)

All Other

All Publications (6 results)

  • [Publications] Satoru Fukiya: "Identification of putative vacuolar serine protease gene in the rice blast fungus, Magnaporthe grisea"Biosci.Biotechnol.Biochem.. 66・4. 663-666 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Takashi Kamakura: "A novel gene CBP1, encoding a putative extracellualr chitin binding protein, may play an important role in the hydrophobic surface sensing of magnaporthe grisea during appressorium differentiation"Mol.Plant-Microbe Interact.. 15・5. 437-444 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ken-ichiro Saitoh: "Targeted gene disruption of the Neuronal Calcium Sensor 1 homologue in rice blast fungus, Magnaporthe grisea"Biosci.Biotechnol.Biochem.. 67・3. 651-653 (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Satoru Fukiya: "Identification of a putative vacuolar serine protease gene in the rice blast fungus, Magnaporthe grisea"Biosci. Biotechnol. Biochem.. 66-4. 663-666 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Takashi Kamakura: "A novel gene CBP1, encoding a putative extracellular chitin binding protein, may play an important role in the hydrophobic surface sensing of Magnaporthe grisea during appressorium differentiation"Mol. Plant-Microbe Interact.. 15-5. 437-444 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ken-ichiro Saitoh: "Targeted gene disruption of the Neuronal Calcium Sensor 1 homologue in rice blast fungus, Magnaporthe grisea"Biosci. Biotechnol. Biochem.. 67-3. 651-653 (2003)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2004-04-14  

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