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2003 Fiscal Year Final Research Report Summary

The basic pathological research for the establishment of the diagnoses of bone tumors and soft tissue tumors using DNA microarray.

Research Project

Project/Area Number 13670164
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Human pathology
Research InstitutionTokyo Medical University

Principal Investigator

MATSUBAYASHI Jun  Tokyo Medical University, Medicine, Assistant, 医学部, 助手 (00338790)

Co-Investigator(Kenkyū-buntansha) MOTOI Toru  Tokyo university hospital, Assistant, 医学部附属病院, 助手 (50291315)
KURODA Masahiko  Tokyo Medical University, Medicine, Assistant professor, 医学部, 講師 (80251304)
Project Period (FY) 2001 – 2003
KeywordsSoft tissue tumor / Bone tumor / Microarray / Chimeric gene / Chromosomal translocation / RT-PCR / Genetic diagnosis
Research Abstract

In this study, we reevaluated about 100 cases of bone tumors and soft tissue tumors whether they had been histologically diagnosed correctly or not. We performed chromosomal analysis and molecular analysis of chromosomal translocations about some cases of diem. For example, a chimeric gene : EWS-ATF1 was identified in all of 8 cases of clear cell sarcoma by RT-PCR. On the basis of the results, we performed the basic experiment for construction of microarray to detect chimeric genes of bone tumors and soft tissue tumors. At first, we examined synovial sarcoma (SS) cases, that were divided into only two subtypes and of which breaking points were simple. We would have independently detected SYT-SSX1, SYT-SSX2 in each SS cases by multiplex RT-PCR method. Moreover, we made a microarray that had three genes: SYT, SSX1, SSX2 and hybridized cDNA fragments amplified by PCR. On the array, the cDNA fragments reacted with the gene arrangement as same as the specific chimeric gene, but didn't reacted with the gene arrangements as same as other chimeric genes. Additionally, we examined EWS-related chimeric genes about EWS-related tunors by both conventional RT-PCR method and multiplex RT-PCR method. As a result, the results by conventional RT-PCR method were as same as the results by multiplex RT-PGR method in many cases. But we couldn't identify target chimeric genes in some cases. And we also had non-specific reactions in some cases. We speculated that these results were due to number of cycle of PCR and reaction temperature. Thus we found, that it is difficult to set up the best condition of the multiplex RT-PCR. But we also found that these results were important and basic data to reach the foundation of the genetic diagnosis of the bone tumors and soft tissue tumors that had chimeric genes, using mnicroarray method.

  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] Shinmura, K.: "Expression of cyclooxygenese-2 in chondroblastoma : immunohistochemical analysis with special emphasis on local inflammatory reaction."Virchows.Archiv.. 444. 28-35 (2004)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hattori, H.: "Human DNA damage checkpoints and their relevance to soft tissue sarcoma."Pathol.Int.. 54. 26-31 (2004)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 元井 亨: "明細胞肉腫のキメラ遺伝子EWS-ATF1:診断への応用"病理と臨床. 21・6. 584-592 (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Domoto, H.: "TLS-CHOP target gene DOL 54 expression in liposarcomas and malignant fobrous histiocytomas."Pathol.Int.. 52. 497-500 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ishida, T.: "Intracortical chondroblastoma mimicking intra-articular osteoid osteoma."Skeletal.Rasiol.. 31. 603-607 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Okamoto, S.: "Extraskeletal myxoid chondrosarcoma. a clinico pathologic, immunohistochemical, and molecular analysis of 18 cases."Hum.Pathol.. 32・10. 1116-1124 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Shinmura, K.: "Expression of cyclooxygenase-2 in chondroblastoma : immunohistochemical analysis with special emphasis on local in flammatory reaction."Virchows.Archiv.. 444. 28-35 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hattori, H.: "Human DNA damage check points and their relevance to soft tissue sarcoma."Pathol.Int.. 54. 26-31 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Motoi, T.: "The chimeric gene of clear cell sarcoma : EWS-ATF-1"Byori to Rinsho. 21-6. 584-592 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Domoto, H.: "TLS-CHOP target gene DOL54 expression in liposarcomas and malignant fibrous histiocytomas."Pathol.Int.. 52. 497-500 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ishida, T.: "Intracortical chondroblastoma mimicking intra-articular osteoid osteoma"Skeletal.Radiol.. 31. 603-607 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Okamoto, S.: "Extraskeletal myxoid chondrosarcoma: a clinicopathologic, immunohistochemical and molecular analysis of 18 cases."Hum.Pathol.. 32-10. 1116-1124 (2001)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2005-04-19  

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