2002 Fiscal Year Final Research Report Summary
Model of Epstein-Barr virus-positive lymphoma with tha background of inflammation
| Project/Area Number |
13670202
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Experimental pathology
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| Research Institution | IWATE MEDICAL UNIVERSITY (2002)
Hokkaido University (2001) |
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Principal Investigator |
KANNO Hiroyuki Iwate Medical Univ. School of Medicine, Assoc. Prof., 医学部, 助教授 (40252663)
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| Project Period (FY) |
2001 – 2002
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| Keywords | Malignant lymphoma / Reactive oxygen species / EB virus / Genetic lesions / Inflammation |
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| Research Abstract |
1. Epstein-Barr virus (EBV)-transformed lymphoblastoid cell line (LCL) acquired the colony-forming capacity on the soft agar through the repeated treatments with reactive oxygen species produced by xanthine / xanthine oxidase system, and several transformed clones were established. Thus, these clones would represent a model of lymphomagenesis by EBV infection in cooperation with inflammation.
2. The expression and genetic changes of cell cycle-related genes (p53, pRB, cyclins, cyclin-dependent kinases, and cyclin-dependent kinase inhibitors) were examined in three colony-forming clones and parent LCL.
(1) The common changes in protein expression among colony-forming clones were not identified with Western blot analyses.
(2) No deletion of tumor suppressor genes, such as p53, pRB, p15, p16, and p21, were not detected by LOH analyses using microsatellite markers close to the genes for heterozygous deletion and by PCR analyses for homozygous deletion.
(3) No mutations were detected in exons 5, 6, 7 and 8 of p53 gene of these clones by direct sequencing.
3. The same analyses as mentioned in item 2 were performed on the two cell lines derived from pyothorax-associated lymphoma. However, no genetic changes were identified.
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[Publications] Qu, W.-M., Miyazaki, T., Terada, M., Okada, K., Mori, S., Kanno, H., and Nose, M.: "A novel autoimmune pancreatitis model in MRL mice treated with polyinosinic : polycytidylic acid"Clinical and Experimental Immunology. 129(1). 27-34 (2002)
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