2001 Fiscal Year Annual Research Report

遺伝子導入によるマラリアを伝播しないハマダラカの作製

Research Project

Project/Area Number	13670253
Research Institution	Jichi Medical University
Principal Investigator	吉田栄人自治医科大学, 医学部, 講師 (10296121)
Co-Investigator(Kenkyū-buntansha)	平井誠自治医科大学, 医学部, 助手 (50326849) 松岡裕之自治医科大学, 医学部, 助教授 (10173816)
Keywords	マラリア / トランスジェニック / 遺伝子導入 / トランスミッション / 一本鎖抗体
Research Abstract	研究代表者(吉田)は2001年7〜8月にかけて英国インペリアル大学において、海外共同研究者であるProf. Sinden, Prof. Crisantiらと共に本研究課題である『遺伝子導入によるマラリアを伝播しないハマダラカ作製』のための予備実験および研究打ち合わせを行った。Prof. Crisantiの研究グループと研究代表者は構築したプラスミドベクター(マラリア原虫の発育阻害をするタンパクをコード)を用い、すでに4系統のトランスジェニック蚊を樹立している。14年度はこれらトランスジェニック蚊のマラリア感受性を研究代表者がインペリアル大学において検討する。またProf. Crisantのグループは、新たなトランスジェニック蚊作製のための新技術(PiggyBac)を開発した(JBC 2002)。さらにハマダラカの強力なプロモーターとしてトリプシンプロモーターの解析を行い、trypsin regulatory elementを同定した(JBC 2001)。共同研究者であるProf. sindenのグループは、蚊のステージで重要な役割を演じているマラリア原虫のP25, P28, pbCHT1分子を同定し、knockout parasiteを作製してその機能を明らかにした(EMBO J 2001, Infect Immun 2001)。最近では、in vitro培養系でマラリア原虫のステージを再現することに成功した(Science 2002)。これらの研究成果は、トランスジェニック蚊研究の本課題の遂行に深く関わっている。

Research Products
(12 results)

All Other

All Publications (12 results)

[Publications] Giannoni F: "Nuclear factors bind to a conserved DNA element that modulates transcription of Anopheles gambiae trypsin genes"J Biol Chem. 276. 700-707 (2001)
[Publications] Schneider J: "A prime-boost immunisation regimen using DNA followed by recombinant modified vaccinia virus Ankara induces strong cellular immune responses against the Plasmodium falciparum TRAP antigen in chimpanzees"Vaccine. 19. 4595-4602 (2001)
[Publications] Mezzasoma L: "Antigen microarrays for serodiagnosis of infectious diseases"Clin Chem. 48. 121-130 (2002)
[Publications] Nolan T: "piggyBac-mediated germline transformation of the malaria mosquito Anopheles stephensi using the red fluorescent protein dsRED as a selectable marker"J Biol Chem. (in press). (2002)
[Publications] Spano F: "The SAG 5 lows of Toxoplasma gondii encodes three novel proteins belonging to the SAG 1 family of surface antigens"Int J Parasitol. 32. 121-131 (2002)
[Publications] Al-Olayan EM: "Complete development of mosquito phases of the malaria parasite in vitro"Science. 295. 677-679 (2002)
[Publications] Gilbert SC: "Enhanced CD8 T cell immunogenicity and protective efficacy in a mouse malaria model using a recombinant adenoviral vaccine in heterologous prime boost immunisation regimes"Vaccine. 20. 1039-1045 (2002)
[Publications] Raibaud A: "Cryofracture electron microscopy of the ookinete pellicle of Plasmodium gallinaceum reveals the existence of novel pores in the alveolar membranes"J Struct Biol. 35. 47-57 (2001)
[Publications] Tomas AM: "P25 and P28 proteins of the malaria ookinete surface have multiple and partially redundant functions"EMBO J. 20. 3975-3983 (2001)
[Publications] Margos G: "Interaction between host complement and mosquito-midgut-stage Plasmodium berghei"Infect Immun. 69. 5064-5071 (2001)
[Publications] Dessens JT: "Knockout of the rodent malaria parasite chitinase pbCHT1 reduces infectivity to mosquitoes"Infect Immun. 69. 4041-4047 (2001)
[Publications] Vlachou D: "Anopheles gambiae laminin interacts with the P25 surface protein of Plasmodium berghei ookinetes"Mol Biochem Parasitol. 112. 229-237 (2001)

2001 Fiscal Year Annual Research Report

遺伝子導入によるマラリアを伝播しないハマダラカの作製

Principal Investigator

吉田 栄人 自治医科大学, 医学部, 講師 (10296121)

Research Products

[Publications] Giannoni F: "Nuclear factors bind to a conserved DNA element that modulates transcription of Anopheles gambiae trypsin genes"J Biol Chem. 276. 700-707 (2001)

[Publications] Schneider J: "A prime-boost immunisation regimen using DNA followed by recombinant modified vaccinia virus Ankara induces strong cellular immune responses against the Plasmodium falciparum TRAP antigen in chimpanzees"Vaccine. 19. 4595-4602 (2001)

[Publications] Mezzasoma L: "Antigen microarrays for serodiagnosis of infectious diseases"Clin Chem. 48. 121-130 (2002)

[Publications] Nolan T: "piggyBac-mediated germline transformation of the malaria mosquito Anopheles stephensi using the red fluorescent protein dsRED as a selectable marker"J Biol Chem. (in press). (2002)

[Publications] Spano F: "The SAG 5 lows of Toxoplasma gondii encodes three novel proteins belonging to the SAG 1 family of surface antigens"Int J Parasitol. 32. 121-131 (2002)

[Publications] Al-Olayan EM: "Complete development of mosquito phases of the malaria parasite in vitro"Science. 295. 677-679 (2002)

[Publications] Gilbert SC: "Enhanced CD8 T cell immunogenicity and protective efficacy in a mouse malaria model using a recombinant adenoviral vaccine in heterologous prime boost immunisation regimes"Vaccine. 20. 1039-1045 (2002)

[Publications] Raibaud A: "Cryofracture electron microscopy of the ookinete pellicle of Plasmodium gallinaceum reveals the existence of novel pores in the alveolar membranes"J Struct Biol. 35. 47-57 (2001)

[Publications] Tomas AM: "P25 and P28 proteins of the malaria ookinete surface have multiple and partially redundant functions"EMBO J. 20. 3975-3983 (2001)

[Publications] Margos G: "Interaction between host complement and mosquito-midgut-stage Plasmodium berghei"Infect Immun. 69. 5064-5071 (2001)

[Publications] Dessens JT: "Knockout of the rodent malaria parasite chitinase pbCHT1 reduces infectivity to mosquitoes"Infect Immun. 69. 4041-4047 (2001)

[Publications] Vlachou D: "Anopheles gambiae laminin interacts with the P25 surface protein of Plasmodium berghei ookinetes"Mol Biochem Parasitol. 112. 229-237 (2001)

吉田栄人自治医科大学, 医学部, 講師 (10296121)