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2002 Fiscal Year Final Research Report Summary

Induction of cellular immunity by oral inoculation of attenuated Salmonella as a live vaccine vector

Research Project

Project/Area Number 13670288
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Bacteriology (including Mycology)
Research InstitutionKitasato University

Principal Investigator

MATSUI Hidenori  Kitasato University, Kitasato Institute for Life Sciences, Assistant Professor, 北里生命科学研究所, 講師 (30219373)

Co-Investigator(Kenkyū-buntansha) EGUCHI Masahiro  Kitasato University, Kitasato Institute for Life Sciences, Research Assistant, 北里生命科学研究所, 助手 (00312215)
NAKAMURA Masahiko  The Kitasato Institute, Center for Basic Research, Researcher, 基礎研究所, 研究員 (30155858)
ISSHIKI Yasunori  Josai University, School of Pharmaceutical Sciences, Research Assistant, 薬学部, 助手 (60312211)
Project Period (FY) 2001 – 2002
KeywordsSalmonella / vaccine vector / immune response
Research Abstract

We evaluated the efficacy of stress response protease gene-deleted mutants as candidates for live oral vaccine strains against Salmonella infection by performing infection studies in mice using a ClpXP or Lon protease-disrupted mutant of Salmonella enterica serovar Typhimurium (S. typhimurium). In vitro, the ClpXP protease regulates flagellum synthesis and the ClpXP-deficient mutant strain exhibits hyper-flagellated bacterial cells. On the other hand, the Lon protease negatively regulates the efficacy to invade epithelial cells and the expression of invasion genes. When five-week-old BALB/c mice were orally administered with 5 x 10^8 colony-forming units (CFU) of the ClpXP- or Lon-deficient strain, bacteria were detected with 10^3 to 10^4 CFU in the spleen, mesenteric lymph nodes, Peyer's patches, and cecum one week after inoculation, and the bacteria then decreased gradually in each tissue. Significant increases of lipopolysaccharide-specific immunoglobulin G (IgG) and secretary IgA (S-IgA) were detected at week 4 and maintained until at least week 12 after inoculation in serum and bile, respectively. Immunization with the ClpXP- or Lon-deficient strain protected mice against oral challenge with the S. typhimurium virulent strain. Both the challenged virulent and immunized avirulent salmonellae were completely cleared from the spleen, mesenteric lymph nodes, Peyer's patches, and even cecum five days after the challenge. These data indicate that the ATP-dependent protease ClpXP- or Lon-disrupted Salmonella can be useful in development a live vaccine strain.

  • Research Products

    (77 results)

All Other

All Publications (77 results)

  • [Publications] M.Nakamura, H.Matsui, et al.: "Persistent increase in myofibroblasts in Helicobacter Helicobacter heilmannii-infected but not in Helicobacter pylori-infected Mongolian gerbil-colocalization of COXII and bFGF immunoreactivity"Aliment. Pharmacol. Ther.. 16(suppl.2). 174-179 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T.Tomoyasu, H.Matsui, et al.: "The ClpPX ATP-dependent protease regulates flagellum synthesis in Salmonella enterica serovar Typhimurium"J. Bacteriol.. 184(3). 645-653 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] A.Kaneko, H.Matsui, et al.: "Association of a regulatory gene, slyA with a mouse virulence of Salmonella serovar Choleraesuis"Microb. Immunol.. 46(2). 109-113 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] H.Tomoda, H.Matsui, et al.: "Purification of Shiga-like toxin 1 by pigeon egg white glycoproteins immobilized on Sepharese gels"Anal. Biochem.. 311(1). 50-56 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] M.Nakamura, H.Matsui, et al.: "Interaction of leptin with gastric myobroblast transdifferentiation in Hp-infected Mongolian gerbil fundic mucosa"Ulcer Research. 29(2). 152-154 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] M.Nakamura, H.Matsui, et al.: "Autonomic innervation of Esophgeal cardial gland -Relation to effector sites of lansoprazole-)"Prog. Med.. 22(3). 753-756 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] A.Takaya, H.Matsui, et al.: "Lon, a stress-induced ATP-dependent protease is critically important for the systemic Salmonella infection in mice"Infect. Immun.. 71(2). 690-696 (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] H.Matsui, et al.: "Oral immunization with ATP-dependent protease-deficient mutants protects mice against subsequent challenge with virulent Salmonella enterica serovar Typhimurium"Infect. Immun.. 71(1). 30-39 (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] M.Nakamura, H.Matsui, et al.: "Autoradiographic and confocal laser microcsopic observation show lafutidine binds to CGRP-immunoreactive nerves as well as gastric parietal cells"Inflammopharmacology. (in press). (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Y.Akiba, M.Nakamura, et al.: "Acid-sensing pathways in rat gastrointestinal mucosa"J. Gastroenterol.. 37(Suppl. XIV). 133-138 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 秋葉保忠, 中村正彦, 石井裕正: "NSAIDSによるラット十二指腸粘液分泌障害と粘膜障害"Ulcer Res.. 29. 155-158 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 中村正彦, 土本寛二: "セロトニン作動薬の作用機序"日本医事新報. 4106. 147-148 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 中村正彦, 土本寛二, 石井裕正: "胃粘膜修復機転からみた線維芽細胞-筋線維芽細胞系の意義-"消化器科. 32(4). 355-359 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 中村正彦, 土本寛二, 秋葉保忠, 石井裕正: "胃粘膜における自律神経の意義-Helicobacter pylori感染と自律神経の関連-"自立神経. 38(2). 144-147 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] M.Nakamura, K.Tsuchimoto, H.Ishii.: "Alcohol and the pancreas"Alcoholism Clinical and Experimental Research. 25. 244S-250S (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 中村正彦: "胃粘膜傷害と自律神経、血管新生因子、成長因子"日本医師会雑誌. 126. M28-M30 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 中村正彦, 土本寛二, 石井裕正: "H. pylori時代の消化性潰瘍学-H. pylori発見20周年を記念して-"日本臨床. 60(Suppl.2). 137-142 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 中村正彦, 土本寛二: "胃酸分泌と壁細胞-H2ブロッカーとPPIの対比-"消火器診療. 55. 12-13 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 中村正彦, 土本寛二, 石井裕正: "消化管ホルモンの動態と酸分泌動態への影響"日本臨床. 60(Suppl.2). 137-142 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 中村正彦, 土本寛二: "胃酸分泌と壁細胞-H2ブロッカーとPPIの対比-"消化器診療. 55. 12-13 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 中村正彦, 土本寛二: "消化器内自律神経および壁在神経は再生するか-増殖因子特にbFGFとの関連-"医学のあゆみ. 201. 67-71 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] M.Eguchi, et al.: "Cytoprotection against ischemia-induced DNA cleavages and cell injuries in the rat liver by pro-vitamine C via hydrolytic conversion into ascorbate"Mol. Cel. Biochem.. (in press). (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] S.Ishikawa, H.Matsui, et al.: "Protection against Shiga toxin 1 challenge by immunization of mice with purified mutant Shiga toxin 1"Infect. Immun.. 71(in press). (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 中村正彦 et al.: "高齢ラットにおける胃、大腸固有節層内glucagon受容体の分布"日本高年齢消化器医学会会誌. 4. 91-92 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 中村正彦, 土本寛二, 秋葉保忠: "実験潰瘍学会との出会い、その後"Ulcer Res.. 29(Suppl.). 138-141 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 中村正彦, 土本寛二: "胃粘膜と微小循環-酸分泌との関連"消化器診療. 50. 10-11 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 中村正彦, 土本寛二: "胃壁在神経叢"消化器診療. 56. 10-11 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 中村正彦, 土本寛二: "胃粘膜防御機構"Annual. Review. 消化器. 121-126 (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Onoue, S., Imai, T., Kumada, H., Umemoto, T., Kaca, W., Isshiki, Y., Kaneko, A., Kawahara, K.: "Serum antibodies of periodontitis patients compared to the lipopolysaccharides of Porphyromonas gingivalis and Fusobacterium nucleatum"Microbiol. Immunol.. 47(1). 51-55 (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] H.Matsui, M.Eguchi, Y.Kikuchi: "Effect of constitutively expressed phoP gene on the localization of Salmonella typhimurium within Mac-1 positive phagocytes"Microbiology and Immunology. 45(1). 79-83 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 中村正彦, 松井英則, 秋葉保忠, 石井裕正: "Hp感染Mongolian gerbil胃粘膜内のIansoprazole結合部位の検討-Iansoprazole 1ヶ月連続投与後の変化-"胃分泌研究会誌. 33. 5-8 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T.Yamamoto, H.Sashinami, A.Takaya, T.Tomoyasu, H.Matsui, Y.Kikuchi, et al.: "Disruption of the genes for clpxp protease in salmonella enterica serovar typhimurium results in persistent infection in mouse, and the development requires endogenous interferon gamma and tumor necrosis factor alpha"Infection and Immunity. 69(5). 3164-3174 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] M.Nakamura, H.Matsui, et al.: "Confocal laser microscopic observsation of H. pylori adhesion to gastrointestinal culture cells"Pcogress in Medicine. 21(3). 587-590 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] H.Matsui, C.M.Bacot, et al.: "The virulence plasmid-borne spvb and spvc genes can replace the 90-kilobase plasmid in conferring virulence to salmonella enterica serovar typhimurium in subcutaneously inoculated mice"Journal of Bacteriology. 183(15). 4652-4658 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] M.Nakamura, T.Akiba, H.Matsui, et al.: "Angiogenesis during healing of acetic acid-induced ulcer : significance of basic fibroblast growth factor"Ulcer Research. 28(1). 5-7 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] M.Nakamura, H.Mataui, et al.: "Alteration of Parietal Cell in Hp-infected Mongolian Gerbil Fundic Mucosa -Relation to Binding Site of H3-cimetidine-"Terapeutic Research. 22(Suppl.1). S59-S63 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 秋葉保忠, 中村正彦, 織田正也, 石井裕正: "酸による十二指腸粘膜上皮細胞傷害におけるion transportの役割"Ulcer Research. 28(2). 139-143 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 143中村正彦, 秋葉保忠, 谷中昭典, 石井裕正: "食道下部粘液腺の構造と機能-PPIの新たな作用部位として-"消化器科. 33(6). 498-500 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 中村正彦, 土本寛二, 石井裕正: "胃粘膜修復機転からみた線維芽細胞-筋線維芽細胞系の意義-"消化器科. 32(4). 355-359 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 中村正彦, 土本寛二, 秋葉保忠, 石井裕正: "胃粘膜における自律神経の意義-Helicobacter pylori感染と自律神経の関連-"自律神経. 38(2). 144-147 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] M.Nakamura, K.Tsuchimoto, H.Ishii: "Alcohol and the pancreas"Alcoholism Clinical and Experimental Research. 25. 244S-250S (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 中村正彦: "胃粘膜傷害と自律神経、血管新生因子、成長因子"日本医師会雑誌. 126. M28-M30 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kawahara K, Lindner B, Isshiki Y, Jakob K, Knirel YA, Zahringer, U.: "Structural analysis of a new glycosphingolipid from the lipopolysaccharide-lacking bacterium Sphingomonas adhaesiva"Carbohydrate Research. 333(1). 87-93 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Isshiki Y, Matsuura M, Dejsirilert S, Ezaki T: "Separation of 6-deoxy-heptan [correction of 6-deoxy-heptane] from the smooth-type lipopolysaccharide preparation of Burkholderia pseudomallei"FEMS Microbiology Letter. 199(1). 21-25 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Shimomura H, Matsuura M, Saito S, Hirai Y, Isshiki Y, Kawahara K: "Lipopolysaccharide of Burknoideria cepacia and its unique ability to stimulate murine macrophages with relative lack of interleukin-ibeta-inducing ability"Infection and Immunnity. 69(6). 3663-3669 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] M.Nakamura, Y.Akiba, H.Matsui, H.Ishii: "Microcirculation Annual"Nihon-Igakukan. 2 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Y.Akiba, M.Nakamura, et al.: "Gastrointestinal function, regulation and Disturbances"Elsevier. 12 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 中村 正彦: "塩基性線維芽細胞増殖因子.Technical Term消化管"先端医学社. 2 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 中村 正彦: "カハール細胞.Technical Term消化管"先端医学社. 2 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Y.Akiba, M.Nakamura, H.Ishii: "Hyperemic response to luminal acid in rat lower intestine via vanilloid receptor-1"Nihon-Igakukan. 2 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 中村 正彦, 松井 英則, 土本 寛二, 秋葉 保忠, 織田 正也, 石井 裕正: "消化管ホルモン(XIX)"医学図書出版株式会社. 2 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hidenori Matsui, Masahiro Eguchi, and Yuji Kikuchi: "Effect of constitutively expressed phoP gene on the localization of Salmonella typhimurium within Mac-1 positive phagocytes"Microbiology and Immunology. 45(1). 79-83 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tomoko Yamamoto, Hiroshi Sashinami, Akiko Takaya, Toshifumi Tomoyasu, Hidenori Matsui, Yuji Kikuchi, Tomoko Hanawa, Shigeru Kamiya, and Akio Nakane: "Disruption of the genes for ClpXP protease in Salmonella enterica serovar Typhimurium results in a persistent infection in mouse and the development requires endogenous interferon-gamma and tumor necrosis factor-alpha"Infection and Immunity. 69(5). 3164-3174 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nakamara Masahiko, Matsui Hidenori, Akiba Yasutada, Kurihara Naoto, Kitajima Masaki, Oda Masaya, and Ishii Hiromasa: "Confocal laser microscopic observation of H. pylori adhesion to gastrointestinal culture cells"Progress in Medicine. 21(3). 587-590 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hidenori Matsui, Christopher M.Bacot, Wendy A.Garlington, Thomas J.Doyle, Steve Roberts, and Paul A.Gulig: "The virulence plasmid-borne spvB and spvC genes can replace the 90-kilobase plasmid in conferring virulence to Salmonella enterica serovar Typhimurium in subcutaneously inoculated mice"Journal of Bacteriology. 183(15). 4652-4658 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Masahiko Nakamura, Yasutada Akiba, Hidenori Matsui, and Hiromasa Ishii: "Angiogenesis during healing of acetic acid-induced ulcer : significance of basic fibroblast growth factor"Ulcer Research. 28(1). 5-7 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Masahiko Nakamura, Hidenori Matsui, Kanji Tsuchimoto, Yasutada Akiba, Masaya Oda, and Hiromasa Ishii: "Effector sites of glucagon in rat stomach observed by radioautography and in situ hybridization method"Digestive hormones. (XIX). 17-19 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Masahiko Nakamura. Hidenori Matsui, Jun Inoue, Yasutada Akiba, and Hiromasa Ishii: "Alteration of Parietal Cell in Hp-infected Mongolian Gerbil Fundic Mucosa-Relation to Binding Site of H3-cimetidine-"Terapeutic Research. 22 (Suppl. 1). S-59-S-63 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Isshiki, Y., Matsuura, M., Dejsirilert, S., Ezaki, T. and Kawahara, K.: "Separation of 6-deoxy-heptan [correction of 6-deoxy-heptane] from the smooth-type lipopolysaccharide preparation of Burkholderia pseudomallei"FEMS Microbiol.Lett.. 199(1). 21-25 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Shimomura, H., Matsuura, M., Saito, S., Hirai Y., Isshiki, Y. and Kawahara, K.: "Lipopolysaccharide of Burkholderia cepacia and its unique ability to stimulate murine macrophages with relative lack of interleukin-1beta-inducing ability"Infect.Immun.. 69(6). 3663-3669 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kawahara, K., Lindner, B., Isshiki. Y., Jacob, K., Knirel, Y.A., and Zahringer, U.: "Structural analysis of a new glycosphingolipid from the lipopolysaccharide-lacking bacterium Sphingomonas adhaesiva"Carbohydr.Res.. 333(1). 87-93 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] M.Nakamura, S.Takahashi, H.Matsui, K.Nishikawa, Y.Akiba, and H.Ishii: "Persistent increase in myofibroblasts in Helicobacter heilmannii-infected but not in Helicobacter pylori-infected Mongolian gerbil-colocalization of COXII and bFGF immunoreactivity"Aliment.Pharmacol.Ther.. 16 (Suppl. 2). 174-179 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T.Tomoyasu, T.Ohkishi, Y.Ukyo, A.Tokumitsu, A.Takaya, M.Suzuki, K.Sekiya, H.Matsui, K.Kutsukake, and T.Yamamoto: "The ClpPX ATP-dependent protease regulates flagellum synthesis in Salmonella enterica serovar Typhimurium"J.Bacteriol.. 184(3). 645-653 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] A.Kaneko, M.Mita, K.Sekiya, H.Matsui. K.Kawahara, and H.Danbara: "Association of a regulatory gene, slyA with a mouse virulence of Salmonella serovar Choleraesuis"Microb.Immunol.. 46(2). 109-113 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] H.Tomoda, M.Arai, N.Koyama, H.Matsui, S.Omura, R.Obata, and Y.C.Lee: "Purification of Shiga-like toxin 1 by pigeon egg white glycoproteins immobilized on Sepbarose gels"Anal.Biochem.. 311(1). 50-56 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] M.Nakamura, Y.Akiba, H.Matsui, and H.Ishii: "Interaction of leptin with gastric myobroblast transdifferentiation in Hp-infected Mongolian gerbil fundic mucosa)"Ulcer Research. 29(2). 152-154 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] M.Nakamura., K.Tsuchimoto, H.Matsui, Y.Akiba, M.Oda, and H.Ishii: "Autonomic innervation of Esophageal cardial gland -Relation to effector sites of lansoprazole-"Prog.Med.. 22(3). 753-756 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Y.Akiba, M.Nakamura, H.Nagata, JD.Kaunitz, and H.Ishii: "Acid-sensing pathways in rat gastrointestinal mucosa"J.Gastroenterol. 37 (Suppl. XIV). 133-138 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] A.Takaya, M.Suzuki, H.Matsui, T.Tomoyasu, and T.Yamamoto: "Lon, a stress-induced ATP-dependent protease is critically important for the systemic Salmonella infection in mice"Infect.Immun.. 71(2). 690-696 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] H.Matsui, M.Suzuki, Y.Isshiki, C.Kodama, M.Eguchi, Y.Kikuchi, K.Motokawa, A.Takaya, T.Tomoyasu, and T.Yamamoto: "Oral immunization with ATP-dependent protease-deficient mutants protects mice against subsequent challenge with virulent Salmonella enterica serovar Typhimurium"Infect.Immun.. 71(1). 30-39 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] M.Nakamura, Y.Akiba, H.Matsui, H.Ishii, and K.Tsuchimoto: "Autoradiographic and confocal laser microscopic observation show lafutidine binds to CGRP-immunoreactive nerves as well as gastric parietal cells"Inflammopharmacology. in press. (2003)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ai Kurita, H.Goto, M.Eguchi, N.Okada, H.Matsui, H.Danbara, and Y.Kikuchi: "Intracellular expression of the Salmonella plasmid virulence protein, SpvB, causes apoptotic cell death in eukaryotic cells"Microb.Pathogen.. in press. (2003)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] M.Eguchi, T.Miyazaki, E.Masatsuji-Kato, T.Tsuzuki, T.Oribe, and N.Miwa: "Cytoprotection against ischemia-induced DNA cleavages and cell injuries in the rat liver by pro-vitsmine C via hydrolytic conversion into ascorbate"Mol.Cel.Biochem.. in press. (2003)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Satoshi Ishikawa, Kazuyoshi Kawahara, Yuta Kagami, Yasunori Isshiki, Aki Kaneko, Hidenori Matsui, Nobuhiko Okada, and Hirofumi Danbara: "Protection against Shiga toxin 1 challenge by immunization of mice with purified mutant Shiga toxin 1"Infection and Immunity. 71, in press. (2003)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] M.Nakamura, Y.Akiba, H.Matsui, and H.Ishii, Eds. M.Asano and C.Ohkubo: "Relation of leptin to microcirculatory rearrangement during Helicobacter pylori-induced gastric mucosal damage"Microcirculation Annual, Vol.18, Nihon-Igakukan. 101-102 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Y.Akiba, M.Nakamura, And H.Ishii, M.Asano and C.Ohkubo, eds.: "Hyperemic response to luminal acid in rat lower intestine via vanilloid receptor-1"Microcirculation Annual, Nihon-Igakukan, Tokyo. 97-98 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Y.Akiba, M.Nakamura, and H.Ishii: "Acid-sensing mechanism and the hyperemic response in rat gastrointestinal mucosa"Gastrointestinal function, regulation and Disturbances 2002 Vol.20, Excepta Medica, Elsevier. 85-96 (2002)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2004-04-14  

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