2002 Fiscal Year Final Research Report Summary
Establishment of animal model of hepatitis C virus infection and examination of vaccine effects.
| Project/Area Number |
13670295
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Virology
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| Research Institution | Mie University |
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Principal Investigator |
YASUTOMI Yasuhiro Mie University, Faculty of Medicine, Associate Professor, 医学部, 助教授 (90281724)
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| Project Period (FY) |
2001 – 2002
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| Keywords | vaccine / immunology / HCV / Adenovirus vector / hepatitis |
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| Research Abstract |
Hepatitis C virus (HCV) is one of the major agents of chronic hepatitis and liver disease worldwide. An animal model for HCV infection is required for both vaccine development and for understanding the pathogenesis of HCV infection. Although the chimpanzee is the only known animal model of HCV infection, chimpanzees are difficult to acquire for laboratory animals. In the present study, we assessed the possibility of infection with recombinant adenovirus (rADV-HCV), which is preferential infection to hepatocytes, having cDNA of HCV structural proteins in mice as a novel animal model. Mice were intravascularly inoculated with rADV-HCV. After rADV-HCV inoculation, concentrations of GPT in sera were increased and much higher than control rADV having cDNA of lacZ. These mice inoculated with rADV-HCV showed acute hepatitis in histopathological examination but not control rADV. These high concentrations of GPT in sera were observed for 2 weeks after infection. The HCV-specific CD8+ cytotoxic T lymphocytes (CTLs) were observed after infection with rADV-HCV. The concentrations of GPT in sera were not increased after infection with rADV-HCV, when FK506 or anti-CD8 monoclonal antibody was administered with mice to eliminate these HCV specific CTLs. These studies demonstrated the capacity of murine model induced by inoculation with rADV-HCV to elicit HCV-specific CTL in association with pathogenesis of HCV infection.
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Research Products
(12 results)
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[Publications] Uno-Furuta, S., Tamaki, S., Takebe, Y., Takamura, S., Kamei, A., Kim, G., Kuromatsu, I., Kaito, M., Adachi, Y., Yasutomi, Y.: "Induction of virus-specific cytotoxic T lymphocytes by in vivo electric administration of peptides"Vaccine. 19. 2190-2196 (2001)
Description
「研究成果報告書概要(和文)」より
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[Publications] Mori, K., Yasutomi, Y., Ohgimoto, S., Nakasone, T., Takamura, S., Shioda, T., Nagai, Y.: "Quintuple Deglycosylation Mutant of Simian Immunodeficiency Virus SIVmac239 in Rhesus Macaques : Robust Primary Replication, Tightly Contained Chronic Infection, and Elicitation of Potent Immunity against the Parental Wild-Type Strain"J. Virol. 75. 4023-4028 (2001)
Description
「研究成果報告書概要(和文)」より
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[Publications] Niikura, M., Takamura, S., Kim, G., Kawai, S., Saijo, M., Morikawa, S., Kurane, I., Tian-Cheng Li, Takeda, N., Yasutomi, Y.: "Chimeric recombinant hepatitis E virus -like particles as an oral vaccine vehicle presenting foreign epitopes"Virology. 273-280 (2002)
Description
「研究成果報告書概要(和文)」より
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[Publications] Imanaka-Yoshida, K., Hiroe, M., Yasutomi, Y., Maki, S., Tsuchiya, T., Noda, N., Toyozaki, T., Nishikawa, T., Ishiyama, S., Sakakura, T., Yoshida, T.: "Tenascin-C is a Useful Marker for Disease Activity in Myocarditis"Journal of Pathology. 197. 388-394 (2002)
Description
「研究成果報告書概要(和文)」より
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[Publications] Mukai, K., Yasutomi, Y., Watanabe, M., Kenjo, A., Aota, T., Wang, L., Nishikawa, H., Fujita, T., Kuribayashi, K., Suzuki, H.: "HER2 peptide-specific CD8+T cells are proportionally detectable long after multiple DNA vaccinations"Gene Therapy. 9. 879-888 (2002)
Description
「研究成果報告書概要(和文)」より
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[Publications] Uno-Furuta, S., Matsuo, K., Tamaki, S., Takamura, S., Kamei, A., Kuromatsu, I., Kaito, M., Matsuura Y., Miyamura, T., Adachi, Y., Yasutomi, Y.: "Immunization with recombinant bacillus Calmette-Guerin (BCG)-hepatitis C virus (HCV) elicits HCV-specific cytotoxic T lymphocytes in mice"Vaccine. (印刷中). (2003)
Description
「研究成果報告書概要(和文)」より
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[Publications] Uno-Furuta,S., Tamaki,S., Takebe,Y., Takamura,S., Kamei,A., Kim,G., Kuromatsu,I., Kaito,M., Adachi,Y.and Yasutomi,Y.: "Induction of virus-specific cytotoxic T lymphocytes by in vivo electric administration of peptides"Vaccine. 19. 2190-2196 (2001)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Mori,K., Yasutomi,Y., Ohgimoto,S., Nakasone,T., Takamura,S., Shioda,T.and Nagai,Y.: "Quintiple deglycosylated mutant of simian immunodeficiency virus SIV mac239 in rhesus macaques : Robust primary replication, tightly contained hronic infection and elicitation of potent immunity against the parental wild-type strain"J.Virol.. 75. 4023-4028 (2001)
Description
「研究成果報告書概要(欧文)」より
-
[Publications] Niikura,M., Takamura,S., Kim,G., Kawai,S., Saijo,M., Morikawa,S., Kurane,I., Tian-Cheng Li, Takeda,N.and Yasutomi,Y.: "Chimeric recombinant hepatitis E virus-like particles as an oral vaccine vehicle presenting foreign epitopes"Virology. 293. 273-280 (2002)
Description
「研究成果報告書概要(欧文)」より
-
[Publications] Imanaka-Yoshida,K., Hiroe,M., Yasutomi,Y., Maki,S., Tsuchiya,T., Noda,N., Toyozaki,T., Noshikawa,T., Ishiyama,S., Sakakura,T.and Yoshida,T.: "Tenascin-C is a Useful Marker for Disease Activity in Myocarditis"J.Pathol.. 197. 388-394 (2002)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Mukai,K., Yasutomi,Y., Watanabe,M., Kenjo,A., Aota,T., Wang,L., Noshikawa,H., Fujita,T., Kuribayashi,K.and Shiku,H.: "HER2 peptide-specific CD8+ T cells are proportionally detectable long after multiple DNA vaccinations"Gene Ther.. 9. 879-888 (2002)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Uno-Furuta,S., Matsuo,K., Tamaki,S., Takamura,S., Kamei,A., Kuromatsu,I., Kaito,M., Matsuura,Y., Miyamura,T., Adachi,Y., and Yasutomi,Y.: "Immunization with recombinant bacillus Calmette-Guerin (BCG)-hepatitis C virus (HCV) elicitis HCV-specific cytotoxic T lymphocytes in mice"Vaccine. in press.
Description
「研究成果報告書概要(欧文)」より
