2002 Fiscal Year Final Research Report Summary
Identification of Y chromosome-linked autoimmune acceleration gene(s)
| Project/Area Number |
13670469
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
内科学一般
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| Research Institution | Jichi Medical School |
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Principal Investigator |
IWAMOTO Masahiro Jichi Medical School, Department of Medicine Assistant Professor, 医学部, 講師 (90291624)
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| Project Period (FY) |
2001 – 2002
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| Keywords | Autoimmunity / Yaa gene(s) |
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| Research Abstract |
The BXSB strain, a recombinant inbred strain derived from a cross between C57BL/6 females and SB/Le males, spontaneously develops an autoimmune syndrome with features of SLE and monocytosis that affects male animals much earlier than females. Studies of reciprocal F_1 hybrids and hone marrow cell transfer experiments have shown that the Yaa gene present in the Y chromosome of the BXSB mouse is responsible for the accelerated autoimmune abnormalities and immunopathological lesions. The positional cloning of Yaa gene is not useful for its existence in the Y chromosome. I tried to clone the Yaa gene by representational difference analysis (RDA). I performed cDNA RDA and genomic DNA RDA methods. Forty-two clones obtained by cDNA RDA using bone marrow cells have been reported else. The intron sequence of SIL (SCL interrupting locus) was obtained by genomic DNA RDA. This difference was thought to be polymorphism of the genome. Bone marrow cell transfer experiment has shown that the product of Yaa gene was expressed on B cells at least. The first cDNA RDA revealed three interesting cDNA clones by which no difference was detected by Northern blot analysis between Yaa mice and non-Yaa mice spleen cells. The second cDNA RDA found the unreported cDNA which had 66 putative amino acids.
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[Publications] Okazaki H, Hirata D, Kamimura T, Sato H, Iwamoto M, Yoshio T, Masuyama J, Fujimura A, Kobayashi E, Kano S, Minota S: "Effects of FTY720 in MRL-lpr/lpr mice : Therapeutic potential in systemic lupus erythematosus"J Rheumatol. 29. 707-716 (2002)
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