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2002 Fiscal Year Final Research Report Summary

Identification of Y chromosome-linked autoimmune acceleration gene(s)

Research Project

Project/Area Number 13670469
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 内科学一般
Research InstitutionJichi Medical School

Principal Investigator

IWAMOTO Masahiro  Jichi Medical School, Department of Medicine Assistant Professor, 医学部, 講師 (90291624)

Project Period (FY) 2001 – 2002
KeywordsAutoimmunity / Yaa gene(s)
Research Abstract

The BXSB strain, a recombinant inbred strain derived from a cross between C57BL/6 females and SB/Le males, spontaneously develops an autoimmune syndrome with features of SLE and monocytosis that affects male animals much earlier than females. Studies of reciprocal F_1 hybrids and hone marrow cell transfer experiments have shown that the Yaa gene present in the Y chromosome of the BXSB mouse is responsible for the accelerated autoimmune abnormalities and immunopathological lesions. The positional cloning of Yaa gene is not useful for its existence in the Y chromosome. I tried to clone the Yaa gene by representational difference analysis (RDA). I performed cDNA RDA and genomic DNA RDA methods. Forty-two clones obtained by cDNA RDA using bone marrow cells have been reported else. The intron sequence of SIL (SCL interrupting locus) was obtained by genomic DNA RDA. This difference was thought to be polymorphism of the genome. Bone marrow cell transfer experiment has shown that the product of Yaa gene was expressed on B cells at least. The first cDNA RDA revealed three interesting cDNA clones by which no difference was detected by Northern blot analysis between Yaa mice and non-Yaa mice spleen cells. The second cDNA RDA found the unreported cDNA which had 66 putative amino acids.

  • Research Products

    (8 results)

All Other

All Publications (8 results)

  • [Publications] Kuroda Y: "Abnormal IgG galactosylation in MRL-lpr/lpr mice"Pathol Int. 51. 909-915 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Okazaki H: "Effects of FTY720 in MRL-lpr/lpr mice"J Rheumotol. 29. 707-716 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kaneko N: "Production of thromboxane A2 and prostaglandin I_2 affected by interaction of heat aggreated IgG, endothelial cells, and platelets in lupus nephntis"J Rheumatol. 29. 2106-2113 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Iwamoto M: "Humanized monodoral anti-Interleukin-6 receptor antibody for treatment of intractable adult-onset still's disease"Arthritis Rheum. 46. 3388-3389 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kuroda Y, Nakata M, Hirose S, Shirai T, Iwamoto M, Izui S, Kojima N, Mizuochi T: "Abnormal IgG galactosylation in MRL-lpr/lpr mice : Pathogenic role in the development of arthritis"Pathol Int. 51. 909-915 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Okazaki H, Hirata D, Kamimura T, Sato H, Iwamoto M, Yoshio T, Masuyama J, Fujimura A, Kobayashi E, Kano S, Minota S: "Effects of FTY720 in MRL-lpr/lpr mice : Therapeutic potential in systemic lupus erythematosus"J Rheumatol. 29. 707-716 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kaneko N, Masuyama J, Nara H, Hirata D, Iwamoto M, Okazaki H, Minota S, Yoshio T: "Production of thromboxane A_2 and prostaglandin I_2 affected by interaction of heat aggregated IgG, endothelial cells, and platelets in lupus nephritis"J Rheumatol. 29. 2106-2113 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Iwamoto M, Nara H, Hirata D, Minota S, Nishimoto N, Yoshizaki K: "Humanized monoclonal anti-interleukin-6 receptor antibody for treatment of intractable adoul-onset Still's disease"Arthritis Rheum. 46. 3388-3389 (2002)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2004-04-14  

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