2002 Fiscal Year Final Research Report Summary
Treatment of pancreatic and biliary cancer using adenovirus whose proliferation isstimulated by radiation
| Project/Area Number |
13670492
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Gastroenterology
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| Research Institution | The University of Tokyo |
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Principal Investigator |
KAWABE Takao The University of Tokyo, Faculty of Medicine, Lecturer, 医学部附属病院, 講師 (40195136)
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| Co-Investigator(Kenkyū-buntansha) |
OOHASHI Makoto The University of Tokyo, Faculty of Medicine, Medical Stuff, 医学部附属病院, 医員
TADA Minoru The University of Tokyo, Faculty of Medicine, Research Associate, 医学部附属病院, 助手 (80302719)
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| Project Period (FY) |
2001 – 2002
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| Keywords | pancreato-biliary / cancer / adenovirus / gene therapy / radiation / in vitro |
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| Research Abstract |
A) Preparation of promoters activated by radiation
Early growth response-1 (egr-1) is known as a promoter activated by radiation. We attempted to make more sensitive mutants for radiation. However, such mutant gene was not obtained.
B) Preparation of adenovirus whose proliferation is stimulated by radiation
As mentioned above, we failed to make more effective promoter. Then, we employed mild egr-1 promoter in the present study. We constructed adenovirus as E1A was regulated by egr-1. Theoretically, radiation activates egr-1 and it activats E1A gene to lead virus proliferation. However, constructed virus did not proliferate by radiation. It was considered because egr-1 did not activate satisfactory E1A gene.
C) Preparation of suicide-gene integrated adenovirus
To avoid uncontrollable proliferation, we integrated suicide-gene system using thymidine kinase from herpes simplex and ganciclovir into the above mention virus. Ganciclovir caused death of the cell infected with this virus.
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[Publications] Akanuma M, Maeda S, Ogura K, Mitsuno Y, Hirata Y, Ikenoue T, Otsuka M, Watanabe T, Yamaji Y, Yoshida H, Kawabe T, Shiratori Y, Omata M.: "The evaluation of putative virulence factor of helicobacter pylori for gastroduodenal disease by use of a short-term Mongolian gerbil infection model."J Infect Dis. 185・3. 341-347 (2002)
Description
「研究成果報告書概要(欧文)」より
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