• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

2002 Fiscal Year Final Research Report Summary

The study of tumor vessel growth inhibiton Angiostatin, new mechanism of action and clinical application

Research Project

Project/Area Number 13670496
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Gastroenterology
Research InstitutionThe University of Tokyo

Principal Investigator

MITSUI Hiroshi  The University of Tokyo, The University of Tokyo Faculty of Medicine, Research (30239280)

Co-Investigator(Kenkyū-buntansha) MARUYAMA Toshiyuki  The University of Tokyo, Faculty of Medicine, Lecturer (30219571)
SAKATA Yoichi  自治医科大学, Jichi Medical School Heamatology, Associate Professor (40129028)
Project Period (FY) 2001 – 2002
KeywordsAngiostain / liver endothelial cells
Research Abstract

Angiostatin (AG) is a product of which a coagulation factor with klingle structure, such as Plasminogen(PL) is cleaved. AG was reported to have capacity to inhibit the growth of cancer cells in vivo. In this study, we examine the mechanism and clinical application of AG. Among the human liver cancer cell lines examined, only Huh-7 cells can cleave purified PL, and produce AG. Human macrophage metalloesterase, a candidate of the cleaving enzyme, is expressed in all cell lines. We next used primary liver endothelial cell culture and add purified AG. From human plasma, PL was purified and then cleaved by elastase, and purified again. Rat liver endothelial cells were purified by collagenase perfusion and percoll centrifugation. AG inhibited endothelial cells growth and the effect depended on bFGF pathway. In another study, 30% of the patients of hepatocellular carcinoma had AG in their plasma judging by Western blotting. We tried immunological staining of liver cancer cell lines and found that HepG2 cells may express ATP synthase, which had been reported as a surface receptor of AG. We will examine that AG could inhibit malignant phenotypical change, such as cadherin E to N type, using this cells.

  • Research Products

    (6 results)

All 2001

All Journal Article (6 results)

  • [Journal Article] The MEK-ERK MAP Kinase pathuay and the P13-Kinase-Akt pathway independently mediate anti-apoptotic signals in HepG2 liver caneor celle2001

    • Author(s)
      Mitsui H., et al.
    • Journal Title

      Int J Cancer 92

      Pages: 55-62

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] IgG_1 anti-P_2 as a marker of response to interferon in patients with chronic hepatitis C2001

    • Author(s)
      Hirayama M, et al.
    • Journal Title

      Clin Exp Immunol 126

      Pages: 92-100

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Esophageal smooth murcle fumor in a 25-year-old woman with congenital malformations2001

    • Author(s)
      Maekawa H., et al.
    • Journal Title

      J Gastroenterol 36

      Pages: 700-703

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] The MEK1-ERK map kinase pathway and the PI 3-kinase-Akt pathway independently mediate anti-apoptotic signals in HepG2 liver cancer cells.2001

    • Author(s)
      Mitsui H, Takuwa N, Maruyama T, Maekawa H, Hirayama M, Sawatari T, Hashimoto N, Takuwa Y, Kimura S.
    • Journal Title

      Int J Cancer. 92(1)

      Pages: 55-62

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] IgGi anti-P2 as a marker of response to interferon in patients with chronic hepatitis C.2001

    • Author(s)
      Hirayama M, Maruyama T, Mitsui H, Maekawa H, Yamada H, Hashimoto N, Koike K, Kimura S, Yasuda K, Iino S, Green J.
    • Journal Title

      Clin Exp Immunol. 126(1)

      Pages: 92-100

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Esophageal smooth muscle tumor in a 25-year-old woman with congenital malformations.2001

    • Author(s)
      Maekawa H, Tanaka N, Hashimoto N, Yamada H, Mitsui H, Ikeda H, Maruyama T, Mori M, Nagawa H, Kimura S.
    • Journal Title

      J Gastroenterol. 36(10)

      Pages: 700-703

    • Description
      「研究成果報告書概要(欧文)」より

URL: 

Published: 2011-06-18  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi