THE ROLE OF HEPATITIS C VIRUS BY INDUCTION OF CARDIOMYOPATHY

2003 Fiscal Year Final Research Report Summary

• Project/Area Number: 13670547
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Gastroenterology
• Research Institution: OSAKA CITY UNIVERSITY
• Principal Investigator: SHIOMI Susumu OSAKA CITY UNIVERSITY, GARDUATE SCHOOL OF MEDICINE, DEPARTMENT OF NUCLEAR MEDICINE, PROFESSOR, 大学院・医学研究科, 教授 (30170848)
• Co-Investigator(Kenkyū-buntansha): YOSHIYAMA Minoru OSAKA CITY UNIVERSITY, GRADUATE SCHOOL OF MEDICINE, DEPARTMENT OF CARDIOLOGY, ASSOCIATE PROFESSOR, 大学院・医学研究科, 助教授 (30240956) ; NISHIGUCHI Shuhei OSAKA CITY UNIVERSITY, GRADUATE SCHOOL OF MEDICINE, DEPARTMENT OF HEPATOLOGY, ASSOCIATE PROFESSOR, 大学院・医学研究科, 助教授 (10192246)
• Project Period (FY): 2001 – 2003
• Keywords: CARDIOMYOPATHY / HEPATITIS C VIRUS / TRANSGENIC MOUSE / HCV CORE PROTEIN / HCV ANTIBODY / HCV GENOTYPE / HCV-RNA / CARDIOMYOCYTE

Research Abstract

Hepatitis C virus (HCV) is the main cause of chronic hepatitis worldwide. HCV has been reported to be associated with cardiomyopathy. However, the mechanism of cardiomyopathy in chronic HCV infection is still unclear. The ability of the core protein of HCV to modulate gene transcription, cell growth and cell death may be involved in the pathogenesis of cardiomyopathy.
Here, we report the development of cardiomyopathy in mice transgenic for the HCV core gene. After the age of 12 months, mice developed cardiomyopathy that appeared as left ventricular dilatation, and systolic and diastolic dysfunction. These cardiac changes were consisted with cardiomyocyte hypertrophy, myofiber disorganization, myofilament loss, myofiber disorganization, Z-band abnormalities, irregular shaped nuclei, increased number of mitochondria and aggregation of mitochondria.
These results indicate that the HCV core protein has an important role in the development of cardiomyopathy, and that these transgenic mice provide good animal models for cardiomyopathy with HCV infection.

Research Products

• [Publications] 西口修平, 他: "循環器疾患におけるHCV抗体陽性率の検討-心筋症との比較"厚生科学研究費・非A非B型肝炎の臨床的総合研究・平成10〜12年度報告書. 160-161 (2002)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Tragaki M, et al.: "Prevalence of hepatitis C virus infection among patients with Hypertrophic cardiomyopathy"Heart & Vessels. 18. 167-170 (2003)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Omura, et al.: "The core protein of hepatitis C virus induced cardiomyopathy in transgenic mice"J Med Virol. (In press).
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] 西口修平, 他: "HCVトランスジェニックマウスにおける心筋症の検討"厚生科学研究費・新興・再感染症研究事業分担研究報告書・平成15年度報告書. (印刷中). (2004)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Nishiguchi S, Fukuda K, Shiomi S, Teragaki M, Yoshiyama M, Yoshikawa j.: "Incidence of hepatitis C virus antibody in patients with hypertrophic cardiomyopathy"Annual Report of Ministry of Health, Labour and Welfare. 160-161 (2002)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Teragaki M, Nishiguchi S, Takeuchi K, Yoshiyama M, Akioka K, Yoshikawa J.: "Prevalence of hepatitis C virus infection among patients with hypertrophic cardiomyopath"Heart Vessels. 18. 167-170 (2003)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Nishiguchi S, Fukuda K, Shiomi S, Teragaki M, Yoshiyama M, Yoshikawa J.: "Evaluation of cardiomyopathy in hepatitis C virus transgenic mouse"Annual Report of Ministry of Health, Labour and Welfare. (in press). (2004)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Omura T, Yoshiyama M, Hayashi T, Nishiguchi S, Fukuda K, Inamoto S, Nakamura y, Teragaki M, Iwao H, Takeuchi K, Yoshikawa J.: "The core protein of hepatitis C virus induced cardiomyopathy in transgenic mice"
  • Description: 「研究成果報告書概要(欧文)」より