2002 Fiscal Year Annual Research Report

結核感染症に対する樹状細胞を用いた次世代ワクチンの開発に関する研究

Research Project

Project/Area Number	13670595
Research Institution	Hamamatsu University School of Medicine
Principal Investigator	須田隆文浜松医科大学, 医学部, 助手 (30291397)
Co-Investigator(Kenkyū-buntansha)	永田年浜松医科大学, 医学部, 助教授 (90275024) 千田金吾浜松医科大学, 医学部, 助教授 (40197611)
Keywords	樹上細胞 / 結核 / ワクチン
Research Abstract	■マウス結核感染症に対するDCを用いたvaccineの有効性を検討するために,以下の抗結核vaccineを作製した. 1)結核菌由来の抗原ペプチド(Ag85A,Ag85B)をcodeしたDNA vaccine 2)結核菌由来の抗原ペプチド(Ag85A,Ag85B)を遺伝子導入したDC 3)結核菌由来の抗原を含むDC exosome ■マウス結核感染モデルにおいて,上記の1),2),3)をvaccineとして接種し,誘導されるprotective immunityの強さを,BCG生菌vaccineと比較した. 1.Ag85A, Bを発現させたplasmidを用いたDNA vaccineを作製する.また,これらのAg85A, BのcDNAを挿入したpMX vectorを retrovirus-producer cell(Phoenix cell)にtransfectionして培養液からretrovirusを回収し,マウス骨髄由来の培養(BALB/c,C57BL)に感染させ遺伝子導入を行った. 2.各種vaccineによるマウスの免疫は,DNA vaccineは金粒子に付着させて,遺伝子銃で皮内に接種,遺伝子導入したDCは静注,DC exosomeは皮内に接種して行った. 3.各種vaccine接種4週間後に,免疫マウスに10^6CFUの結核菌を静注し,経時的に脾,肺内の結核菌の菌量を測定する.また,経気道的にも同量の結核菌を吸入させて経時的に肺内菌量を測定する.vaccineによって誘導されるprotective immunityの強さは臓器内菌量で評価し,無免疫マウスとBCG生菌を接種したマウスと比較した.

Research Products

(6 results)

All Other

All Publications (6 results)

[Publications] Matsuda H, Suda T, et al.: "Alteration of balance between myeloid dendritic cells and plasmacytoid dendritic cells in peripheral blood of asthmatic patients"Am J Respir Crit Care Med. 165. 1050-1054 (2002)
[Publications] Tsuchiya T, Suda T, et al.: "Dendritic cell Involvement in pulmonary granuloma formation elicited by BCG in rats"Am J Respir Crit Care Med. 165. 1640-1646 (2002)
[Publications] Suda T, et al.: "Oncostatin M production by human dendritic cells in response to bacterial products"Cytokine. 17. 335-340 (2002)
[Publications] Nakamura Y, et al.: "Induction of Protective Immunity Against Listeria monocytogenes Using Dendritic Cells Retrovirally Transduced with Listeriolysin O"Infect Immun. 71(in press). (2003)
[Publications] Uchiyama H., et al.: "Endosomal/lysosomal targeting of a single helper T-cell epitope of an intracellular bacterium by DNA immunization induces a specific T-cell subset and partial ptotective immunity in vivo"FEMS Microbiol. 216. 91-97 (2002)
[Publications] Hayakawa H., et al.: "Clinicopathological features of chronic haypersensitivity pneumonitis"Resprology. 7. 359-364 (2002)

2002 Fiscal Year Annual Research Report

結核感染症に対する樹状細胞を用いた次世代ワクチンの開発に関する研究

Principal Investigator

須田 隆文 浜松医科大学, 医学部, 助手 (30291397)

Research Products

[Publications] Matsuda H, Suda T, et al.: "Alteration of balance between myeloid dendritic cells and plasmacytoid dendritic cells in peripheral blood of asthmatic patients"Am J Respir Crit Care Med. 165. 1050-1054 (2002)

[Publications] Tsuchiya T, Suda T, et al.: "Dendritic cell Involvement in pulmonary granuloma formation elicited by BCG in rats"Am J Respir Crit Care Med. 165. 1640-1646 (2002)

[Publications] Suda T, et al.: "Oncostatin M production by human dendritic cells in response to bacterial products"Cytokine. 17. 335-340 (2002)

[Publications] Nakamura Y, et al.: "Induction of Protective Immunity Against Listeria monocytogenes Using Dendritic Cells Retrovirally Transduced with Listeriolysin O"Infect Immun. 71(in press). (2003)

[Publications] Uchiyama H., et al.: "Endosomal/lysosomal targeting of a single helper T-cell epitope of an intracellular bacterium by DNA immunization induces a specific T-cell subset and partial ptotective immunity in vivo"FEMS Microbiol. 216. 91-97 (2002)

[Publications] Hayakawa H., et al.: "Clinicopathological features of chronic haypersensitivity pneumonitis"Resprology. 7. 359-364 (2002)

須田隆文浜松医科大学, 医学部, 助手 (30291397)