2002 Fiscal Year Final Research Report Summary
Cytogenetically analysis of congenital abnormality and childhood malignancy using multicolor FISH methods
| Project/Area Number |
13670808
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Pediatrics
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| Research Institution | HIROSHIMA UNIVERSITY |
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Principal Investigator |
TASHIRO Satoshi Hiroshima University, Graduate School of Biomedical Sciences, Assistant Professor, 大学院・医歯薬学総合研究科, 講師 (20243610)
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| Co-Investigator(Kenkyū-buntansha) |
IGARASHI Kazuhiko Hiroshima University, Graduate School of Biomedical Sciences, Professor, 大学院・医歯薬学総合研究科, 教授 (00250738)
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| Project Period (FY) |
2001 – 2002
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| Keywords | Color banding FISH method / childhood leukemia |
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| Research Abstract |
We established Color banding FISH method for the precise cytogenetical analysis, and started collection of clinical sample in 2001.
We are testing the ways to transfer clinical sample to Prof. Michael Speeches, a collaborator, for multicolor FISH analysis of these clinical samples in Munich. Clinical samples of childhood leukemia are provided from "Japan Association of Childhood Leukemia Study, J*****.
To identify new genes associated with childhood leukemia by the new method combining color banding FISH method and conventional FISH method to detect a specific gene, we have done functional analysis of some candidate genes. Among them, we have revealed that BACH2, a B cell specific transcription regulator, showed induction of apoptosis upon oxidative stress. Furthermore, we also found that Bach2 is expressed not only in B cell but also in brain.
We have established a method to study the dynamics of chromosome in living cell to study the mechanism of chromosome translocation with Prof. Thomas Cremer, a Collaborator in Munich, and study the dynamics of chromosome territories using method.
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Research Products
(10 results)
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[Publications] Sun J., Hoshino H., Takaku K., Nakajima O., Muto A., Suzuki H., Tashiro S., Takahashi S., Shibahara S., Alam J., Taketo MM. Yamamoto M., lgarashi K.: "Hemoprotein Bach1 regulates enhancer availability of heme oxygenase-1 gene"EMBO J.. 21(19). 5126-5224 (2002)
Description
「研究成果報告書概要(欧文)」より
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