2001 Fiscal Year Annual Research Report

^<31>P―MRSと低酸素細胞イメージングによる腫瘍内再酸素化機構の解析

Research Project

Project/Area Number	13670918
Research Institution	Gunma University
Principal Investigator	桜井英幸群馬大学, 医学部, 講師 (50235222)
Keywords	低酸素細胞 / 腫瘍 / 放射線 / 再酸素化 / MR Spectroscopy / ^<31>P-MRS
Research Abstract	腫瘍の酸素状態を非侵襲的に測定できれば,放射線治療や化学療法といった治療法の選択だけでなく,その最適な投与量や投与間隔を予測する事が可能である.すなわち治療後の組織の再構築や個々の腫瘍細胞の再酸素化を指標とした腫瘍の個別化が可能と考えられ,最も効果的な抗腫瘍効果を得る治療法を選択できる可能性がある.近年,第2,3世代の低酸素細胞マーカーが開発され,生体内の低酸素細胞を非侵襲的に画像化し,定量することが可能となりつつある.本研究では,これまでの^<31>P-MRSやMRIによるperfusion imageにくわえて,低酸素細胞マーカーによる低酸素細胞イメージングを用いて腫瘍の酸素状態を推定し,腫瘍の再酸素化を指標として生物学的に最適な放射線治療法について模索することを目的とした.マウス移植腫瘍を用いて,種々の低酸素測定法から得られたデータと腫瘍重量との関係を検討した.またそれぞれの方法で定量した値から,推定される放射線感受性について相関関係を算出した.新規低酸素細胞マーカーであるβ-D-IAZGPは血液中の濃度よりも約8倍のマーカーの集積を認め,正常臓器よりも有意な集積が認められた.また腫瘍重量が増加するほどマーカーは良好に集積する傾向が認められた.^<31>P-MRSによるβ-ATP/Pi及びPCr/Piは腫瘍重量が増加するほど低値を示した.またin vivo vitro assayでは腫瘍重量が増加すると放射線抵抗性が誘導されることが明らかとなった.また低酸素細胞マーカーにより放射線照射後の再酸素化を非侵襲的にとらえられる可能性が示唆され,今後の研究課題と考えられた.

Research Products

(12 results)

All Other

All Publications (12 results)

[Publications] Sakurai H.: "Analysis of recurrence of squamous cell carcinoma of the uterine cervix after definitive radiation therapy alone patterns of recurrence, latent periods and prognosis"Int. J. Radiat. Oncol. Biol. Phys.. 50・5. 1136-1144 (2001)
[Publications] Sakurai H.: "Clinical application of low dose-rate brachytherapy combined with simultaneous mild temperature hyperthermia"Anticancer Res.. 21. 679-684 (2001)
[Publications] Sakurai H.: "A challenge for Non-small cell lung cancer using hyperthermia"Jpn. J. Hyperthermic Oncol.. 171. 23-31 (2001)
[Publications] Akimoto T.: "Genistein, a tyrosine kinase inhibitor, enhanced radiosensitivity in human esophageal cancer cell lines in vitro : possible involvement of inhibition of survival signal transduction pathways"Int. J. Radiat. Oncol. Biol. Phys.. 50・1. 195-201 (2001)
[Publications] Ishikawa H.: "The effects of p53 status and human papillomavirus infection on the clinical outcome of patients with stage IIIB cervical carcinoma treated with radiation therapy alone"Cancer. 91・1. 80-89 (2001)
[Publications] Hayakawa K.: "High-dose radiation therapy for elderly patients woth inoperable or unresectable non-small cell lung canner"Lung Cancer. 32. 81-88 (2001)
[Publications] Islam MS: "Hyperthermia-induced apoptosis in two rat yolk sac tumor cell lines with different radiothermosensitivity in vitro"Oncology Reports. 8. 501-407 (2001)
[Publications] Kurosaki H.: "Biological cell survival mapping for radiofrequency intracavitary hyperthermia combined with simultaneous high dose-rate intracavitary irradiation"Jpn.J.Cancer Res. 92・1. 95-102 (2001)
[Publications] Tsukiyama I.: "Multi-institutional phase I and II trial of simultaneous intra-cavitary hyperthermia and brachytherapy for advanced esophageal cancer"J Brachytherapy International. (in press).
[Publications] Imai R.: "Small cell lung cancer with a brain metastasis controlled for 5 years : a case report"Jpn J Clin Oncol. 31・3. 116-118 (2001)
[Publications] Fujita K.: "The combined effect of preoperative hyperthermia and radiation therapy on advanced rectal cancer"Jpn J of Hyperthermic Oncol.. 17・3. 151-159 (2001)
[Publications] Mogi A.: "Intrathoracic thermochemotherapy for the treatment of post-operative malignant pleural effusion in primary lung carcinoma-Report of a cese"Jpn J of Hyperthermic Oncol.. 17・3. 159-164 (2001)

2001 Fiscal Year Annual Research Report

^<31>P―MRSと低酸素細胞イメージングによる腫瘍内再酸素化機構の解析

Principal Investigator

桜井 英幸 群馬大学, 医学部, 講師 (50235222)

Research Products

[Publications] Sakurai H.: "Analysis of recurrence of squamous cell carcinoma of the uterine cervix after definitive radiation therapy alone patterns of recurrence, latent periods and prognosis"Int. J. Radiat. Oncol. Biol. Phys.. 50・5. 1136-1144 (2001)

[Publications] Sakurai H.: "Clinical application of low dose-rate brachytherapy combined with simultaneous mild temperature hyperthermia"Anticancer Res.. 21. 679-684 (2001)

[Publications] Sakurai H.: "A challenge for Non-small cell lung cancer using hyperthermia"Jpn. J. Hyperthermic Oncol.. 171. 23-31 (2001)

[Publications] Akimoto T.: "Genistein, a tyrosine kinase inhibitor, enhanced radiosensitivity in human esophageal cancer cell lines in vitro : possible involvement of inhibition of survival signal transduction pathways"Int. J. Radiat. Oncol. Biol. Phys.. 50・1. 195-201 (2001)

[Publications] Ishikawa H.: "The effects of p53 status and human papillomavirus infection on the clinical outcome of patients with stage IIIB cervical carcinoma treated with radiation therapy alone"Cancer. 91・1. 80-89 (2001)

[Publications] Hayakawa K.: "High-dose radiation therapy for elderly patients woth inoperable or unresectable non-small cell lung canner"Lung Cancer. 32. 81-88 (2001)

[Publications] Islam MS: "Hyperthermia-induced apoptosis in two rat yolk sac tumor cell lines with different radiothermosensitivity in vitro"Oncology Reports. 8. 501-407 (2001)

[Publications] Kurosaki H.: "Biological cell survival mapping for radiofrequency intracavitary hyperthermia combined with simultaneous high dose-rate intracavitary irradiation"Jpn.J.Cancer Res. 92・1. 95-102 (2001)

[Publications] Tsukiyama I.: "Multi-institutional phase I and II trial of simultaneous intra-cavitary hyperthermia and brachytherapy for advanced esophageal cancer"J Brachytherapy International. (in press).

[Publications] Imai R.: "Small cell lung cancer with a brain metastasis controlled for 5 years : a case report"Jpn J Clin Oncol. 31・3. 116-118 (2001)

[Publications] Fujita K.: "The combined effect of preoperative hyperthermia and radiation therapy on advanced rectal cancer"Jpn J of Hyperthermic Oncol.. 17・3. 151-159 (2001)

[Publications] Mogi A.: "Intrathoracic thermochemotherapy for the treatment of post-operative malignant pleural effusion in primary lung carcinoma-Report of a cese"Jpn J of Hyperthermic Oncol.. 17・3. 159-164 (2001)

桜井英幸群馬大学, 医学部, 講師 (50235222)