2002 Fiscal Year Final Research Report Summary
BASIC RESEARCH OF HIGH INTENSITY FUCUSED ULTRASOUND FOR HEPATIC TUMOR-EVALUATION OF TERATMENT EFFECT-
| Project/Area Number |
13670954
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Radiation science
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| Research Institution | SAPPORO MEDICAL UNIVERSITY |
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Principal Investigator |
KOITO Kazumitsu SAPPORO MEDIDAL UNIVERSITY, SCHOOL OF MEDICINE, ASSISTANT PROFESSOR, 医学部, 講師 (90153460)
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| Co-Investigator(Kenkyū-buntansha) |
NAGAKURA Hisayasu SAPPORO MEDIDAL UNIVERSITY, SCHOOL OF MEDICINE, INSTRUCTOR, 医学部, 助手 (80244359)
HAREYAMA Masato SAPPORO MEDIDAL UNIVERSITY, SCHOOL OF MEDICINE, PROFESSOR, 医学部, 教授 (10173098)
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| Project Period (FY) |
2001 – 2002
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| Keywords | focused ultrasound / hepatocellular carcinoma / apoptosis |
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| Research Abstract |
1. Histopathological evaluation of high intensity focused ultrasound (HIFU) for normal skin and hepatic tumors.
HIFU was performed on the skin of the rats (five rats). Resected specimen of the skin and implanted KDH hepatoma in the skin of the rats. After irradiation of HIFU showed necrosis and sever inflammatory changes around the region. In addition, cell vacuolation and consendation of the nuclei were observed. Those findings were observed in both the normal skin and the implanted tumor.
2. Evaluation of HIFU for implanted WKAH hepatoma
HIFU was performed on implanted WHAH/HKm hepatoma of rats (mean diameter 3.6 mm). Complete necrosis was obtained by using 30w x 1.0 sec energy. On the contrary, no necrosis was obtained by less than 10w energy. HIFU group showed significantly (p<.05) longer survival compared with non-treatment group.
3. Apoptosis manifestation after HIFU
HIFU irradiaton was performed on culture cells of KDH-8 hepatoma. Immediately after that, apoptosis cell were stained by hekist 33258 fluorescence and were counted by the fluorescent microscope. Apoptosis was significantly (p<.05) appeared in the group of 30w energy more than one of 40w.
Those studies indicated that 1) HIFU induces necrosis of implanted hepatoma, 2) treatment effect depends on energy of HIFU, and 3) HIFU induces apoptosis by using appropriate energy.
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Research Products
(12 results)
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[Publications] Koh-ichi Sakata, Yoshihisa Matsumoto, Hiroshi Tauchi, Masaaki Satoh, Atsushi Ouchi, Hisayasu Nagakura, Kazumitsu Koito, Yoshio Hosoi, Norio Suzuki, Kenshi Komatsu, Masato Hareyama: "Expression of genes involved in repair of DNA double-strand breaks in normal and tumor tissues"Int J Radiat Oncol Biol Phys. 49(1). 161-167 (2001)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Koh-ichi Sakata, Yoshihisa Matsumoto, Hiroshi Tauchi, Masaaki Satoh, Atsushi Ouchi, Hisayasu Nagakura, Kazumitsu Koito, Yoshio Hosoi, Norio Suzuki, Kenshi Komatsu, Masato Hareyama: "Expression of genes involved in repair of DNA double-strand breaks in normal and tumor tissues"Int J Radiat Oncol Biol Phys. 49(1). 161-167 (2001)
Description
「研究成果報告書概要(欧文)」より
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