Relationship between Rho family protein expression and ability of invasiveness of breast cancer cells on their process to lose estrogen dependency

2002 Fiscal Year Final Research Report Summary

• Project/Area Number: 13671239
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General surgery
• Research Institution: TFE University of Tokushima
• Principal Investigator: KOMAKI Kansei University of Tokushima, university hospital, Assistant Professor, 医学部附属病院, 講師 (60215382)
• Co-Investigator(Kenkyū-buntansha): MASUDA Eitarou University of Tokushima, university hospital, Assistant, 医学部附属病院, 助手 (50314860) ; KONDO Kazuya University of Tokushima, school of medicine, Assistant Professor, 医学部, 講師 (10263815)
• Project Period (FY): 2001 – 2002
• Keywords: Rho A / Rho B / Rac-1 / estrogen receptor / DMBA induced breast cancer

Research Abstract

[Expression of Rho family proteins in human breast concer-relation to ER status and histologic grade pf malignancy] Generally, Rho family proteins were weakly expressed in human invasive ductal carcinoma of the bresst. Ns Though ER prote expression inversely related to Rac-1 expression, which had no related to Rho B expressions. Between Rho family protein expression, there was significant relation in Rac-1 and Rho A, and expression of Rho A protein related to the degree of tubular formation of the tumors.
[Relationship between expression of Rho family and ability of tumor in DMBA induced rat breast cancer] Concerning with the expression of Rho family proteins in DMBA induced rat brest cancer, Rho A protein and Rac-1 protein showed strong ex pression at the time that began to palpate tumors, and these proteins expression decreased at 16 weeks later from that time. However, a statistical significance was not able to confirm in the changes of these protains expression. There was no constant tendency in the expresson of Rho B during the growing in DMBA induced rat tumor. In addition, as for the expression of estrogen receptor, a decreasing tendency inclued expression same as a conventional examination result. With Rho A, Rac-1, expression was similar and paralleled in DMBA rat breast cancer. Tumor invasiveness at 16 weeks was significantly strong in comparison with the beginning of tumor palpable. In conclusion, Rho A and Rac-1 proteins expression was decreased same as estrogen receptor expression according as the tumor growing, where the ability of invasivness was increased.