2002 Fiscal Year Final Research Report Summary
Development of Bio Sensor Chip Assay
| Project/Area Number |
13671332
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Digestive surgery
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| Research Institution | Iwate Medical University School of Medicine |
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Principal Investigator |
ISHIDA Kaoru Iwate Medical University School of Medicine, Department of Surgery, Assistant Professor, 医学部, 助教授 (70146041)
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| Co-Investigator(Kenkyū-buntansha) |
MAESAWA Chihaya Iwate Medical University School of Medicine, Department of Pathology, Lecturer, 医学部, 講師 (10326647)
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| Project Period (FY) |
2001 – 2002
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| Keywords | Biomolecular / Interaction analysis / Bio sensor / esophageal cancer / cancer related genes |
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| Research Abstract |
We developed a new system for predicting disease outcome of cancer patients, by using Bio Sensor Chip assay. The assay utilized the surface plasmon resonance technology for the analysis Biomolecular interaction analysis. To overcome the disadvantages of the conventional telomerase assay (TRAP : telomeric repeat amplification protocol) related to PCR artifacts and troublesome post-PCR procedures, we have developed a telomeric repeat elongation (TRE) assay which directly measures telomerase activity as the telomeric elongation rate by biosensor technology using surface plasmon resonance. The assay is rapid and sensitive method, and moreover, allows precise quantitative comparison of a wide range of human cells from somatic cells to carcinoma cells. TRE assay is suitable for practical use in the assessment of telomerase activity in preclinical and clinical trials of telomerase-based therapies, because of its reproducibility, rapidity and simplicity.
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[Publications] Fujiwara, H., Terashima, M., Irinoda, T., Takagane, A., Abe, K., Kashiwaba, M., Oyama, K., Takahashi, M., Maesawa, C., Saito, K., Takechi, T., and Fukushima, M.: "Quantitative Measurement of Thymidylate Synthase and Dihydropyrimidine Dehydrogenase mRNA Level in Gastric Cancer by Real-time RT-PCR"J Cancer Res. 93. 1342-1350 (2002)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Nakamura, R., Obara, T., Katsuragawa, S., Tamakawa, Y., Koeda, K., Ikeda, K., Sato, N., Ishida, K., Shozushima, M.: "Failure in presumption of residual disease by quantification of FDG uptake in esophageal squamous cell carcinoma immediately after radiotherapy"
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「研究成果報告書概要(欧文)」より
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[Publications] Iijima, S., Maesawa, C., Sato, N., Ikeda, K., Inaba, T., Akiyama, Y., Ishida, K., Saito, K., and Masuda, T.: "Gastrointestinal stromal tumor of the oesophagus : significance of immunohistochemical and genetic analyses of the c-kit gene"Eur J Gastroenterol Hepatol. 14. 445-448 (2002)
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「研究成果報告書概要(欧文)」より
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[Publications] Maesawa, C., Iijima, S., Sato, N., Yoshinori, N., Suzuki, M., Tarusawa, M., Ishida, K., Tamura, G., Saito, K., and Masuda, T.: "Esophageal extraskeletal Ewing's sarcoma"Hum Pathol. 33. 130-132 (2002)
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「研究成果報告書概要(欧文)」より
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[Publications] Maesawa, C., Inaba, T., Sato, H., Iijima, S., Ishida, K., Terashima, M., Sato, R., Suzuki, M., Yashima, A., Ogasawara, S., Oikawa, H., Sato, N., Saito, K., and Masuda, T.: "A rapid biosensor chip assay for measuring of telomerase activity using surface plasmon resonance"Nucleic Acids Res. 31. E4--4 (2003)
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「研究成果報告書概要(欧文)」より
