2002 Fiscal Year Final Research Report Summary
Mechanism of myocacardial preconditioning against ischemia reperfusion injury and effective means of improving function of heart transplants
| Project/Area Number |
13671389
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Thoracic surgery
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| Research Institution | Tottori University |
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Principal Investigator |
ISHIGURO Shingo Tottori University, Faculty of Medicine assistant professor, 医学部, 助教授 (70212868)
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| Co-Investigator(Kenkyū-buntansha) |
OHGI Shigetsugu Tottori University, Faculty of Medicine, professor, 医学部, 教授 (50168845)
NAKAMURA Yoshinobu Tottori University, University hospital, assistant, 医学部附属病院, 助手 (80335538)
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| Project Period (FY) |
2001 – 2002
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| Keywords | intra-cellular calcium overload / ischemia reperfusion injury / preconditioning / myocardial protection / heart preservation |
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| Research Abstract |
Cardiac harvest teams are usually committed immediately transfer the explanted donor heart into its cold storage solution. We tested the opposite hypothesis that brief prestorage episode of ischemic preconditioning could be protected. This study was designed to study the effect of ischemic preconditioning in an isolated heart model of cold storage, simulating the situation of cardiac of cardiac allograft. Isolated isovolumic buffer-perfused rat hearts in normothermic Klebs-Henseleitsolution were arrested with and storage with cold Klebs-Henseleit solution at 4℃ for 4hours before a 1-hour reperfusion. Control hearts were arrested with and stored in K-H solution. Preconditioning group hearts were preconditioned by two 5 minutes episodes of global ischemia, each separated by 5 minutes of reperfusion before arrested with and stared in K-H solution. On the basis of comparisons of postischemic left ventricular contractility and diastolic function, and heart rate, values indicative of improved protection were obtained by ischemic preconditioning These data suggest that cardioprotection by ischemic preconditioning might effectively contribute to improve donor heart preservation during cardiac transplantation.
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Research Products
(4 results)
