New metastasis regulated genes from human lung carcinoma to functional analysis and application for therapy

2002 Fiscal Year Final Research Report Summary

• Project/Area Number: 13671404
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Thoracic surgery
• Research Institution: Dokkyo University School of Medicine
• Principal Investigator: YASUDA Shin-ichi Dokkyo University, School of Medicine, Lecture, 医学部, 講師 (60133279)
• Co-Investigator(Kenkyū-buntansha): NAGAI Sensuke Dokkyo University, School of Medicine, Associated Professor, 医学部, 講師 (10118482)
• Project Period (FY): 2001 – 2002
• Keywords: lung carcinoma / ectropic inoculation / tumor metastasis regulated gene

Research Abstract

A metastasis from primary tumor is concluded through many processes. It is the important problem, which relates to the patient's prognosis closely and to make this analysis of the therapy for the cancer. We established the lung cancer cell line, which it only metastasis to the lung. Subtraction was carried out between metastasis and non-metastasis cells. We analyzed four genes, which related to the metastasis. Laminin receptor (LR) gene is specific receptor toward cell adhesion protein to laminins. It was proved that there was a loss of exson in a part of the gene that a code does a 67kDa molecule as an analysis. And, PAF-AH is acetylhydlase of the activated factor of the phospholipids (PAF), which produced of the platelets, which relates to the tumor metastasis. It is involved in the movement of the nerve cell, but the cancer metastasis isn't clear. Participation is suggested to the metastasis control due to inactivation of PAF due to the expression of this PAF-AH gene with the non-metastasis line. Furthermore, RACE method was enforced, and it got the gene of the extension to analyze two unknown genetic fragments. These genes are related to the melanoma, one of others existed in 14 chromosome, which relates to the uterine cervix carcinoma and the intestine mucous membrane protein, and agreement were entry of Gene bank with it recently The genes are presumed probably to be the molecule, which relates to the natural metastasis mechanism but couldn't get the functional events for metastasis.